采用冷冻聚合法制备了多孔结构的 P(NI-PAm-co-AAm)智能水凝胶，选用牛血清白蛋白(BSA)为模型药物分子，通过后包裹技术负载蛋白质药物，考察了多孔水凝胶中蛋白质药物的载药量和体外释放行为，研究了不同干燥处理方法对载药后水凝胶的药物控释性能的影响，并且与传统方法制备的 P(NIPAm-co-AAm)水凝胶进行了药物控释性能对比实验。实验结果表明，在凝胶中引入多孔结构使得 P(NIPAm-co-AAm)水凝胶的药物载药量和释放量得到了显著的提高。不同的干燥处理方法对多孔P(NIPAm-co-AAm)水凝胶的药物释放影响很大，载药后的凝胶采用冰箱冷冻干燥处理，可使蛋白质药物有较好的缓释效果。
Porous poly (N-isopropylamide-co-acrylamide)[P (NIPAm-co-AAm)]hydrogels were prepared by freezing polymerization method.With bovine serum albumin (BSA)as the model drug,it was loaded into por-ous P (NIPAm-co-AAm)hydrogel by post-fabrication encapsulation technique.The loading efficiency and re-lease ratio of model drug were investigated.The influence of different dry processing method on the controlled drug release properties of the drug-loaded hydrogel was also studied.The results showed that creating an inter-connecting pore structure within the hydrogel matrices was an effective strategy to improve the drug loading ef-ficiency and the cumulative released amount.In vitro BSA release from the hydrogels exhibited that dry pro-cessing method played a dominant role in the controlled drug release properties of P(NIPAm-co-AAm)hydro-gel.The drug-loaded hydrogel with the refrigerator freeze-drying treatment showed better sustained drug re-lease of protein drugs.
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