采用荧光光谱法和紫外光谱法比较研究了新型含空间链四-(6-氨基己酸磺肽基)铝氯酞菁(AlPc (SO2 NHC5 H10 COOH)4)及AlPc(SO2 NHC5 H10 COOH)4/牛血清白蛋白(BSA)复合物的光物理性质。与无空间链四羧基铝氯酞菁(AlPc(COOH)4)相比较,AlPc (SO2 NHC5 H10 COOH)4不易聚集,荧光强度增强,荧光寿命增长。当 AlPc(SO2 NHC5 H10 COOH)4和 Al-Pc(COOH)4分别与BSA形成复合物后,与相应的自由酞菁相比,AlPc (COOH )4/BSA 和 AlPc (SO2-NHC5 H10 COOH)4/BSA复合物的紫外光谱红移,荧光光谱猝灭,其荧光猝灭原因为静态猝灭。由于空间链效应,AlPc(SO2 NHC5 H10 COOH)4与 BSA 之间的作用明显强于AlPc(COOH)4与BSA之间的作用。
The purpose of this study was to investigate the photophysical properties of 3rd generation photosensi-tizer using in photodynamic therapy.The photophysical properties of tetra-carboxyl-aminohexanoic-sulfonyl phthalocyanine aluminium chloride (AlPc(SO2NHC5 H10COOH)4)-bovine serum albumin (BSA)bioconjugate was studied by UV-Vis and fluorescence spectroscopic method. The results indicated AlPc (SO2 NHC5 H10 COOH)4 exhibited lower aggregation,higher fluorescence intensity and longer lifetime compared with tetra-carboxyl phthalocyanine aluminium chloride (AlPc(COOH)4 )which may due to the spacer hindrance effect.When AlPc(SO2NHC5 H10COOH)4 and AlPc(COOH)4 conjugates with BSA,compared with the corre-sponding free phthalocyanine,the UV-Vis spectra of AlPc(COOH)4/BSA and AlPc(SO2 NHC5 H10 COOH)4/BSA was red shift and fluorescence spectra was quenched.Furthermore,AlPc(SO2NHC5 H10COOH)4 conju-gate also showed stronger combining interaction with bovine serum albumin than that of AlPc(COOH)4 due to the spacer hindrance effect.
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