利用阳极氧化法在纯 Ti 表面制得 TiO2纳米管,随后用冻干法在纳米管中负载 microRNA 制得复合涂层。利用 SEM、TEM和荧光显微镜对 TiO2/microRNA复合涂层进行表征,并对 microRNA 的释放行为以及其进入成骨细胞后的功效进行分析。结果表明,microRNA分子成功负载在 TiO2纳米管中,并在12 h内持续稳定释放投递,荧光照片表明投递的microRNA成功进入成骨细胞,ALP 活性测定进一步表明进入细胞中 microRNA促进了成骨分化。
TiO2 nanotubes was prepared by anodic oxidation method on pure Ti surface,then the microRNA was loaded in the nanotubes by lyophilization to build composite coating.The TiO2/microRNA composite coating was characterized by SEM,TEM and fluorescence microscope.The release behavior of microRNA and its furthrer function was analysised.The results showed that microRNA successfully deposited in TiO2 nanotubes, and sustained release delivery for 1 2 h.Fluorescent images indicated that the microRNA was transfected into osteoblasts,ALP activity assay further indicated that the microRNA promoted the osteogenic differentiation.
参考文献
| [1] | Monroig P D;Calin G A .MicroRNA and epigenetics:di-agnostic and therapeutic opportunities[J].Curr Pathobi-ol Rep,2013,1:43-52. |
| [2] | Gopinath M. Sundaram;John E. A. Common;Felicia E. Gopal;Satyanarayana Srikanta;Krishnaswamy Lakshman;Declan P. Lunny;Thiam C. Lim;Vivek Tanavde;E. Birgitte Lane;Prabha Sampath .'See-saw' expression of microRNA-198 and FSTL1 from a single transcript in wound healing[J].Nature,2013(Mar.7 TN.7439):103-106. |
| [3] | Meijer H A;Kong Y W;Lu W T et al.Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation[J].SCIENCE,2013,340:82-85. |
| [4] | Lian,J.B.;Stein,G.S.;VanWijnen,A.J.;Stein,J.L.;Hassan,M.Q.;Gaur,T.;Zhang,Y. .MicroRNA control of bone formation and homeostasis[J].Nature reviews. Endocrinology,2012(4):212-227. |
| [5] | Zhang J;Tu Q;Bonewald LF;He X;Stein G;Lian J;Chen J .Effects of miR-335-5p in modulating osteogenic differentiation by specifically downregulating Wnt antagonist DKK1.[J].Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research,2011(8):1953-1963. |
| [6] | Li,Y.;Fan,L.;Liu,S.;Liu,W.;Zhang,H.;Zhou,T.;Wu,D.;Yang,P.;Shen,L.;Chen,J.;Jin,Y. .The promotion of bone regeneration through positive regulation ofangiogenic-osteogenic coupling using microRNA-26a[J].Biomaterials,2013(21):5048-5058. |
| [7] | Suh,J.S.;Lee,J.Y.;Choi,Y.S.;Chong,P.C.;Park,Y.J. .Peptide-mediated intracellular delivery of miRNA-29b for osteogenic stem cell differentiation[J].Biomaterials,2013(17):4347-4359. |
| [8] | Zhang Xinming;Li Qiang;Zhu Shengli et al.Research developments of bio-medical Ti-Nb based alloys[J].Ma-terials Review,2012,26(03):96-100. |
| [9] | 王文革,邹兴政,王宏,李方,张十庆,丁渝红,王东哲.一种新型医用高氮无镍不锈钢的生物相容性研究[J].功能材料,2013(16):2362-2366. |
| [10] | 林修洲,蔡振兵,易敬华,任平弟,龚敏,朱旻昊.saline溶液中钛合金扭动微动运行区域研究[J].功能材料,2013(15):2179-2183. |
| [11] | Palmieri, A;Pezzetti, F;Brunelli, G;Zollino, I;Lo Muzio, L;Martinelli, M;Scapoli, L;Arlotti, M;Masiero, E;Carinci, F .Zirconium oxide regulates RNA interfering of osteoblast-like cells[J].Journal of Materials Science. Materials in Medicine,2008(6):2471-2476. |
| [12] | Chakravorty S;Helb D;Burday M;Connell N;Alland D .A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria[J].Journal of Microbiological Methods,2007(2):330-339. |
| [13] | Kun Liang;Xiao Cheng Li;Beng Kang Tay .Study of Bone Morphogenetic Protein-2 Delivery with Different TiO_2 Nanotube Structures[J].Nanoscience and Nanotechnology Letters,2013(2):162-166. |
| [14] | Dimitrakopoulou, D.;Rethemiotaki, I.;Frontistis, Z.;Xekoukoulotakis, N. P.;Venieri, D.;Mantzavinos, D..Degradation, mineralization and antibiotic inactivation of amoxicillin by UV-A/TiO<sub>2</sub> photocatalysis.[J].Journal of Environmental Management,2012:168-174. |
| [15] | Kaitainen, S.;M?h?nen, A.J.;Lappalainen, R.;Kr?ger, H.;J Lammi, M.;Qu, C. .TiO_2 coating promotes human mesenchymal stem cell proliferation without the loss of their capacity for chondrogenic differentiation[J].Biofabrication,2013(2):025009-1-025009-12. |
| [16] | Gomez-Florit M;Rubert M;Ramis J M et al.TiO2 Scaffolds sustain differentiation of MC3T3-E1 cells[J].J Biomater Tiss Eng,2012,2:336-344. |
| [17] | Shuting Lai;Wei Zhang;Fang Liu;Cui Wu;Dongping Zeng;Yongxue Sun;Yuehua Xu;Yueping Fang;Wuyi Zhou .TiO_2 Nanotubes as Animal Drug Delivery System and In Vitro Controlled Release[J].Journal of nanoscience and nanotechnology,2013(1):91-97. |
| [18] | Kim M. Kummer;Erik Taylor;Thomas J. Webster .Biological Applications of Anodized TiO_(2) Nanostructures: A Review from Orthopedic to Stent Applications[J].Nanoscience and Nanotechnology Letters,2012(5):483-493. |
| [19] | Kim D;Macak JM;Schimidt-Stein F;Schmuki P .Capillary effects, wetting behavior and photo-induced tube filling of TiO2 nanotube layers[J].Nanotechnology,2008(30):305710-1-305710-5-0. |
| [20] | Servant A;Methven L;Williams R P et al.Electrore-sponsive polymer-carbon nanotube hydrogel hybrids for pulsatile drug delivery in vivo[J].Advanced Healthcare Materials,2013,2:806-811. |
| [21] | Claus Moseke;Felix Hage;Elke Vorndran;Uwe Gbureck .TiO_2 nanotube arrays deposited on Ti substrate by anodic oxidation and their potential as a long-term drug delivery system for antimicrobial agents[J].Applied Surface Science: A Journal Devoted to the Properties of Interfaces in Relation to the Synthesis and Behaviour of Materials,2012(14):5399-5404. |
| [22] | Dou C;Marti G P;Liu L et al.Transfection of murine dermis via topical DNA plasmid delivery[J].WOUND REPAIR AND REGENERATION,2013,21:A21-A21. |
| [23] | Wu K M;Xu J;Liu M Y et al.Induction of osteogenic differentiation of stem cells via a lyophilized microRNA reverse transfection formulation on a tissue culture plate[J].Int J Nanomed,2013,8:1595-1607. |
| [24] | Kim HK;Cho SG;Kim JH;Doan TK;Hu QS;Ulhaq R;Song EK;Yoon TR .Mevinolin enhances osteogenic genes (ALP, type I collagen and osteocalcin), CD44, CD47 and CD51 expression during osteogenic differentiation.[J].Life sciences,2009(9/10):290-295. |
| [25] | 王小红,曹阳,刘钟馨,李士普,林仕伟.TiO2纳米管尺寸对其生物活性的影响[J].功能材料,2013(04):517-521,526. |
| [26] | Hassan M Q;Maeda Y;Jafferji M et al.MicroRNA-218 and Wnt signaling regulatory loop promote osteo-blast differentiation[J].BONE,2012,50:S80-S81. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%