以 N-羟基琥珀酰亚胺(NHS)和1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC· HCl)为催化剂，将制备的双羧基聚乙二醇(COOH-PEG-COOH)和部分巯基化聚乙烯亚胺(PEI-SHx )先后接枝于壳聚糖分子，得到巯基化壳聚糖衍生物(CS-PEG-PEI)。利用傅立叶红外光谱仪(FT-IR)和核磁共振仪(1 H NMR)对其结构进行表征；通过透析法制备自聚集纳米粒，透射电子显微镜(TEM)和动态激光粒度分析仪(DLLS)分析测试自聚集纳米粒显示，自聚集纳米粒为球状纳米胶束，该纳米球有望成为纳米药物及基因的载体。
Thiolated chitosan derivative (CS-PEG-PEI)was synthesized by grafting double carboxy-terminated PEG and partly thiolated polyethylenimine (PEI-SHx)onto chitosan with NHS/EDC as catalyst.The chemical structure of polymer was characterized by the Fourier transform infrared spectroscopy(FT-IR)and proton nu-clear magnetic resonance(1 H NMR),the self-assembled nanoparticles was prepared by probe sonication-dialysis method.The measurements of transmission electron microscope(TEM)and dynamic light scattering(DLS)in-dicated that the self-aggregates were nanoparticles.This novel nanoparticle system was promising to be a good drug carrier and gene carrier.
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