采用静电纺丝法以六氟异丙醇(HFIP)为溶剂,将质量比为70∶30的 SF/COL (丝素/胶原)和PLLC(聚左旋乳酸己内酯)共混制备质量比为0∶100,30∶70,50∶50,70∶30,100∶0的8% SF/COL/PLCL的三维纳米纤维支架,25%戊二醛蒸汽交联48 h后检测各组比例支架材料的理化性能及生物性能。扫描电镜显示纳米纤维形貌良好,均为纳米级别；X射线衍射结果显示交联后材料结晶度增强,Silk Ⅰ构象由α-折叠形成稳定的β-折叠；材料交联后为疏水性, PLCL的加入明显改善材料的亲水性能；热稳定性随着PLCL浓度的增加也愈加稳定,交联后尤其明显。细胞培养实验证明,随着培养时间延长,细胞在不同比例的支架上粘附、铺展和增殖均良好,但 hPDLSCs 与配比为30∶70的 SF/COL/PLCL 支架复合后增殖速率最快。
The aim of this study was tostructure a kind of three-dimensiona nanofiber scaffold composed of SF, collagenⅠand PLCL by using electrospinning technique and to evaluate its performance.We took 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP)as solvent to prepare different mass ratios of SF/collagenⅠ/PLCL scaffolds (0∶100,30∶70,50∶50,70∶30,100∶0)and the properties of these scaffolds were studied before and after they were crosslinked by 25% glutaraldehyde vapor for 48 hours.The results were described as follow:scan-ning electron microscopy(SEM)and the contact water angle meter analysis showed that the uniformity of com-posite nanofibers was improved and with the existence of PLCL,the hydrophilic property decreased.XRD anal-ysis indicates that the nanofibers scaffolds had higher crystallinity after crosslinked.The thermal stability of the scaffold material was also increased.With rising levels of PLCL,the properties of the scaffolds were increased significantly after crosslinking.When the human periodontal ligament stem cells(hPDLSCs)were seeded on SF/collagenⅠ/PLCL scaffolds,they grew well and showed better ability of adhesion and proliferation.Among the scaffolds,PLCL content of 70% did best with significant differences.Our study indicated that this kind of electrospun three-dimensiona nanofiber scaffold might be a potential candidate for restoration of periodontal de-fect and the periodontal tissue engineering.
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