研究利用造孔剂法制备高度贯通的多孔羟基磷灰石(H A)支架,孔隙率约为78%,并利用聚己内酯(PCL)分别复合纳米 HA(nHA)或微纳米生物玻璃(nBG)粉末对其进行涂覆改性,粉末的添加量均为10%~40%(质量分数)。4种类型支架分别记为 HA、PCL/HA、nHA-PCL/HA 和 nBG-PCL/HA。实验结果发现,nHA-PCL/HA 和 nBG-PCL/HA 复合支架最大抗压强度分别为1.41~1.98 MPa 和1.35~1.78 MPa。4类支架矿化实验显示,浸泡21 d 后nBG-PCL/HA表面促进生成较多的磷灰石矿化物；细胞实验结果显示细胞在4类支架上均生长良好,说明支架具有良好的生物相容性。支架在实验犬背部肌肉组织内植入2个月的组织学检测显示,4种支架内均有新骨形成,尤其是 nHA-PCL/HA和 nBG-PCL/HA孔内有更多的新生骨组织,说明这两种支架表面复合涂层中的生物活性纳米颗粒对诱导新骨生成具有积极的促进作用。
Highly interconnected porous hydroxyapatite (HA)scaffolds with high porosity (≈78%)were pre-pared by particle leaching method in the present work,and subsequently the as-prepared HA scaffolds were lined with PCL/nano-HA (nHA)or PCL/micro-nano-bioglass (nBG)composite coatings,the contents of the powder were varied from 10% to 40wt%.The four types of scaffolds were signed as HA,PCL/HA,nHA-PCL/HA and nBG-PCL/HA.The mechanical testing results showed that compressive strengths of nHA-PCL/HA and nBG-PCL/HA were 1.41-1.98 MPa and 1.35-1.78 MPa,respectively.The biomineralization results indicated that much apatite was formed on the surface of nBG-PCL coated scaffolds after immersed in SBF for 2 1 d.Cell culture experimental results showed that osteoblasts grew well on all scaffolds,indicating that all scaffolds possessed positive biological compatibility.Histological section evaluation showed that there was a large amount of new bone forming in nHA-PCL/HA and nBG-PCL/HA scaffolds comparing to other two scaf-folds after implanted for 2 months in the back muscle tissue of dogs,which exhibited that the two biological particles in composite coatings played a positive role in the induction of new bone formation at non-osseous sites.
参考文献
| [1] | Wang Hao;Zhi Wei;Lu Xiong et al.Comparative stud-ies on ectopic bone formation in porous hydroxyapatite scaffolds with complementary pore structures[J].ACTA BIOMATERIALIA,2013,9:8413-8421. |
| [2] | Dehghani, Fariba;Annabi, Nasim . .Engineering porous scaffolds using gas-based techniques[J].Current opinion in biotechnology,2011(5):661-666. |
| [3] | Sicchieri LG;Crippa GE;de Oliveira PT;Beloti MM;Rosa AL .Pore size regulates cell and tissue interactions with PLGA-CaP scaffolds used for bone engineering.[J].Journal of tissue engineering and regenerative medicine,2012(2):155-162. |
| [4] | Wei G;Ma PX .Macroporous and nanofibrous polymer scaffolds and polymer/bone-like apatite composite scaffolds generated by sugar spheres.[J].Journal of biomedical materials research, Part A,2006(2):306-315. |
| [5] | 赵婧,李金雨,智伟,鲁雄,贾治彬,翁杰.蜡球造孔法制备多孔HA陶瓷支架及其性能优化[J].无机材料学报,2013(01):74-78. |
| [6] | Preparation of bioactive porous HA/PCL composite scaffolds[J].Applied Surface Science: A Journal Devoted to the Properties of Interfaces in Relation to the Synthesis and Behaviour of Materials,2008(5 Pt.2):2942. |
| [7] | Kouhi M;Morshed M;Varshosaz J .Poly(e-caprolac-tone)incorporated bioactive glass nanoparticles and simv-astatin nanocomposite nanofibers:Preparation,character-ization and in vitro drug release for bone regeneration ap-plications[J].CHEMICAL ENGINEERING JOURNAL,2013,228:1057-1065. |
| [8] | 焦燕,吕宇鹏,王爱娟,李士同.特殊形态羟基磷灰石的制备及研究进展[J].生物骨科材料与临床研究,2009(02):47-51. |
| [9] | Roohani Esfahani SI;Nouri Khorasani S;Lu ZF;Appleyard RC;Zreiqat H .Effects of bioactive glass nanoparticles on the mechanical and biological behavior of composite coated scaffolds.[J].Acta biomaterialia,2011(3):1307-1318. |
| [10] | 潘明珠,梅长彤.纳米SiO2-APP对木塑复合材料界面特性及力学性能的影响[J].北京林业大学学报,2013(05):117-122. |
| [11] | 魏庆华,汪焰恩,杨明明,魏生民.HA/PLA复合材料界面相互作用及其力学性能的MD模拟[J].功能材料,2013(21):3089-3093,3098. |
| [12] | Kokubo T;Takadama H .How useful is SBF in predicting in vivo bone bioactivity?[J].Biomaterials,2006(15):2907-2915. |
| [13] | Hench L .Sol-gel materials for bioceramic applications[J].Current Opinion in Solid State & Materials Sci-ence,1997,2:604-610. |
| [14] | Tamjid, E.;Bagheri, R.;Vossoughi, M.;Simchi, A. .Effect of particle size on the in vitro bioactivity, hydrophilicity and mechanical properties of bioactive glass-reinforced polycaprolactone composites[J].Materials science & engineering, C. Biomimetic and supramolecular systems,2011(7):1526-1533. |
| [15] | 轩肖娜,朱海霖,陈建勇.丝素蛋白/纳米生物玻璃复合多孔支架的结构与性能[J].功能材料,2013(02):226-231. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%