为了对比甲醇、戊二醛两种交联剂对丝素蛋白/明胶复合多孔支架的性能影响,采用冷冻干燥法等比例制备该支架,分两组分别用甲醇和戊二醛进行交联。通过观察支架的微观形貌,测量支架的孔隙率、吸水率、溶胀率,测试热稳定性及力学性能,比较经两种交联剂处理后支架结构和性能的变化。结果表明,经戊二醛交联后的支架孔隙分布更加规则、均匀,孔隙率、吸水率、溶胀率更高,力学性能更强。采用戊二醛交联丝素蛋白/明胶复合多孔支架,能够使支架性能更加优良。
This paper compares the effects of methanol and glutaraldehyde on properties of silk fibroin/gelatin composite porous scaffolds,the silk fibroin/gelatin composite porous scaffolds with equal proportion were pre-pared by freeze-drying method,cross-linked by methanol and glutaraldehyde respectively.Microstructures of scaffolds were observed;porosities,water absorption ratios and swelling ratios were measured;thermal stabili-ties and mechanical properties were investigated.Then differences of scaffolds,structures and properties by dif-ferent cross linking agents were compared.Results showed that scaffolds cross-linked by glutaraldehyde had a more regular,uniform pore distribution,higher porosities,water absorption ratios,swelling ratios and me-chanical properties.Glutaraldehyde-crosslinked silk fibroin/gelatin composite porous scaffolds,properties are more excellent.
参考文献
[1] | Bhardwaj, Nandana;Kundu, Subhas C. .Electrospinning: A fascinating fiber fabrication technique[J].Biotechnology Advances,2010(3):325-347. |
[2] | Bhardwaj,N.;Kundu,S.C. .Chondrogenic differentiation of rat MSCs on porous scaffolds of silk fibroin/chitosan blends[J].Biomaterials,2012(10):2848-2857. |
[3] | Kim UJ;Park J;Joo Kim H;Wada M;Kaplan DL .Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin.[J].Biomaterials,2005(15):2775-2785. |
[4] | Figallo E;Flaibani M;Zavan B et al.Micropatterned biopolymer 3D scaffold for static and dynamic culture of human fibroblasts[J].Biotecnology Progress,2007,23(01):210-216. |
[5] | Zhang X;Reagan MR;Kaplan DL .Electrospun silk biomaterial scaffolds for regenerative medicine.[J].Advanced drug delivery reviews,2009(12):988-1006. |
[6] | Rockwood, D.N.;Preda, R.C.;Yücel, T.;Wang, X.;Lovett, M.L.;Kaplan, D.L. .Materials fabrication from Bombyx mori silk fibroin[J].Nature protocols erecipes for researchers,2011(10):1612-1631. |
[7] | John G. Hardy;Thomas R. Scheibel .Composite materials based on silk proteins[J].Progress in Polymer Science,2010(9):1093-1115. |
[8] | Liu X;Smith LA;Hu J;Ma PX .Biomimetic nanofibrous gelatin/apatite composite scaffolds for bone tissue engineering.[J].Biomaterials,2009(12):2252-2258. |
[9] | Malafaya PB;Silva GA;Reis RL .Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications.[J].Advanced drug delivery reviews,2007(4/5):207-233. |
[10] | Qiang Lu;Xiaohui Zhang;Xiao Hu;David L. Kaplan .Green Process to Prepare Silk Fibroin/Gelatin Biomaterial Scaffolds[J].Macromolecular bioscience,2010(3):289-298. |
[11] | Waree Tiyaboonchai;Pratthana Chomchalao;Sutatip Pongcharoen .Preparation and Characterization of Blended Bombyx mori Silk Fibroin Scaffolds[J].Fibers and Polymers,2011(3):324-333. |
[12] | Chomchalao P;Pongcharoen S;Sutheerawattananonda M.Fibroin and fibroin blended three-dimensional scaffolds for rat chondrocyte culture[J].BioMedical En-gineering on Line,2013(12) |
[13] | Xiao Li,Jiankang He,Yaxiong Liu,Qian Zhao,Wanquan Wu,Dichen Li,Zhongmin Jin.Biomaterial Scaffolds with Biomimetic Fluidic Channels for Hepatocyte Culture[J].仿生工程学报(英文版),2013(01):57-64. |
[14] | 殷丽华,彭鹏,牟星,程文晓,黄嵩涛,余占海.丝素/明胶三维多孔支架的构建及其结构和性能表征[J].功能材料,2013(23):3388-3391. |
[15] | Kang HW;Tabata Y;Ikada Y .Fabrication of porous gelatin scaffolds for tissue engineering.[J].Biomaterials,1999(14):1339-1344. |
[16] | Jetbumpenkul, P.;Amornsudthiwat, P.;Kanokpanont, S.;Damrongsakkul, S. .Balanced electrostatic blending approach - An alternative to chemical crosslinking of Thai silk fibroin/gelatin scaffold[J].International Journal of Biological Macromolecules: Structure, Function and Interactions,2012(1):7-13. |
[17] | Jianglin Wang;Qin Yang;Chuanbin Mao;Shengmin Zhang .Osteogenic differentiation of bone marrow mesenchymal stem cells on the collagen/silk fibroin bi-template-induced biomimetic bone substitutes.[J].Journal of biomedical materials research, Part A,2012(11):2929-2938. |
[18] | Zhu Hailin;Wu Binwei;Feng Xinxing et al.Prepara-tion and characterization of bioactive mesoporous calcium silicate-silk fibroin composite films[J].J Biomed Mater Res Part B:Appl Biomater,2011,98B(02):330-341. |
[19] | van Luyn M J;van Wachem P B;Damink L O et al.Relations between in vitro cytotoxicity and crosslinked dermal sheep collagens[J].Biomed Mater Res,1992,26(08):1091-1110. |
[20] | Meinel L;Fajardo R;Hofmann S et al.Silk implants for the healing of critical size bone defects[J].BONE,2005,37(05):688-698. |
[21] | 刘明,闵思佳,朱良均.冷冻干燥对丝素蛋白凝胶结构的影响[J].蚕业科学,2007(02):246-249. |
[22] | 张洁,徐烈.冻干支架孔隙形态的影响因素[J].低温工程,2002(02):38-43. |
[23] | Yan,L.-P.;Oliveira,J.M.;Oliveira,A.L.;Caridade,S.G.;Mano,J.F.;Reis,R.L. .Macro/microporous silk fibroin scaffolds with potential for articular cartilage and meniscus tissue engineering applications[J].Acta biomaterialia,2012(1):289-301. |
[24] | Mandal BB;Kundu SC .Cell proliferation and migration in silk fibroin 3D scaffolds.[J].Biomaterials,2009(15):2956-2965. |
- 下载量()
- 访问量()
- 您的评分:
-
10%
-
20%
-
30%
-
40%
-
50%