采用o/w型乳化-溶剂挥发法来制备载药微球,以二氯甲烷为溶剂相,以聚乳酸为载体材料,以维A酸为包埋药物,以吐温-80和明胶为乳化剂.探索载药微球制备过程中的变量(高剪切转速、高剪切时间、内外相体积比、壁材用量等)对载药微球粒径大小、包封率以及稳定性等的影响.得出最优载药微球制备方案：明胶浓度7．5 mg/mL,吐温浓度6 mg/mL,聚乳酸浓度10 mg/mL,内外相体积比1∶10,剪切时间30 min,搅拌速度300 r/min,挥发时间3 h.所制得的聚乳酸载药微球形态光滑且分散性较好,包封率为52．42％.
In this study,o/w emulsion-solvent evaporation method was used to make microspheres,using dichlo-romethane as the solvent phase,polylactic acid as the carrier material and vitamin A acid as the entrapped ob-ject,tween 80 and gelatine as emulsifier.The technical conditions such as shear speed,shear time,internal,ex-ternal phase volume ratio and the amount of wall material were determined.The experiments demonstrated that the optimal conditions were:gelatin concentration of 7 .5 mg/mL,tween concentration of 6 mg/mL,polylactic acid concentration of 10 mg/mL,inside and outside the phase volume ratio of 1∶10,shearing time of 30 min, stirring speed of 300 r/min,volatile time of 3 h.The final product was smooth and well dispersed,and the en-capsulation efficiency was 52.42%.
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