以可溯源性猪皮为原料,采用特殊的化学、物理及生化方法对猪皮进行高度纯化处理,通过观察实验中皮块厚度、面积、废液中胶原蛋白与非胶原蛋白含量及组织学形貌变化,得出脱细胞猪真皮基质碱膨胀的最佳方案为双氧水4％-氢氧化钠6％.并制备了高纯度脱细胞猪真皮基质,检测其抗张强度为7．2 MPa,吸湿性能好,不含重金属,无经口急毒,细胞除去干净且细胞毒性1级,为今后脱细胞猪真皮基质在生物医用材料领域的应用奠定基础.
Using traceable pigskin as raw material,by observing the change of thickness,area,collagen content, non-collagen content in effluent and the histological morphology,the optimum alkali swelling scheme,H 2 O 2 4%-NaOH 6%,of preparing acellular dermal matrix has been harvested.After the preparation of highly-puri-fied porcine-derived acellular dermal matrix,a series of tests have been carried out.The tensile strength of the material was 7.2 MPa.It has good water absorption ability and no heavy metal.Animal experiments demonstra-ted no obvious acute peroral toxicity and the cytotoxicity was only 1 grade.These results lay the foundation for the application of the highly-purified porcine-derived acellular dermal matrix in biomedical materials area in the future with further research.
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