以草鱼皮为原料提取天然胶原蛋白,经DSC、圆二色谱和 SDS-PAGE 分析证实为具有完整三螺旋分子结构的Ⅰ型胶原.在不同条件下对该胶原进行热处理后分别获得了低变性、高变性和完全变性的胶原样品,热变性焓测定结果表明热变性程度分别为16．9％,57．9％和100％.在此基础上,重点研究了热变性处理后胶原结构和胶原海绵材料性能的变化.结果表明,草鱼皮胶原变性的起始温度(To,33℃)是其分子结构和胶原海绵材料性能改变的阀值温度.高于该温度条件下的短暂受热历史即可导致胶原三螺旋结构的部分或完全解离.同时,胶原变性程度的增加,材料结构的有序性、材料力学性能和耐酶解能力也随之降低,但是促细胞增殖能力有所提升.可见,低于胶原热变性的起始温度是天然胶原提取和胶原材料应用的安全温度条件.
In this paper,native collagen (NC)was extracted from skin of grass carp,and it was proved typeⅠcollagen with intact triple helix structure via the DSC and circular dichroism and SDS-PAGE analysis.Low ther-mal denaturation (LD),high thermal denaturation (HD)and complete thermal denaturation(CD)collagen sam-ples were obtained by heat treatment of NC under different conditions,and the degrees of thermal denaturation of them were 16.9%,57.9 and 100% respectively,according to the changes of thermal denaturation enthalpy from DSC determination.On this basis,the research focused on the influences of heat treatment on the collagen structure and the corresponding properties of collagen sponges.The results show that the onset endothermic temperature (To )is the threshold temperature for the molecule structure and sponges properties stability. When subj ected to temperatures above this threshold temperature even in a short time,the triple helix structure of collagen would completely or partially unfold.At the same time,the ordering of material structure,mechani-cal properties and enzymatic sensitivities of collagen sponges reduced,the cell proliferation ability enhanced a-long with the rise of degrees of thermal denaturation of collagen.The findings demonstrate To is the crucial temperature and this temperature should not be exceeded during the production and utilization of native colla-gens.
参考文献
| [1] | Birk D E;Bruckner P .Collagen suprastructures[J].Top-ics in Current Chemistry,2005,247:185-205. |
| [2] | Friess W .Collagen-biomaterial for drug delivery[J].Eu-ropean Journal of Pharmaceutics and Biopharmaceutics,1998,45:36-113. |
| [3] | Zeng S K;Zhang C H;Lin H et al.Isolation and charac-terization of acid-solubilised collagen from the skin of nile tilapia(oreochromis niloticus)[J].Food Chemistry,2009,116:879-883. |
| [4] | Swatschek Dieter;Schatton Wolfgang;Kellermann Josef;Muller WernerE G;Kreuter Jorg .Marine sponge collagen: isolation, characterization and effects on the skin parameters surface-pH, moisture and sebum.[J].European journal of pharmaceutics and biopharmaceutics: official journal of Arbeitsgemeinschaft fuer Pharmazeutische Verfahrenstechnik e.V,2002(1):107-113. |
| [5] | Yasuyuki Kawaguchi;Eiji Kondo;Nobuto Kitamura;Kazunobu Arakaki;Yasuhito Tanaka;Masanobu Munekata;Nobuhiro Nagai;Kazunori Yasuda .In vivo effects of isolated implantation of salmon-derived crosslinked atelocollagen sponge into an osteochondral defect[J].Journal of Materials Science. Materials in Medicine,2011(2):397-404. |
| [6] | Lin Y K;Liu D C .Comparison of physical-chemical prop-erties of type Ⅰ collagen from different species[J].Food Chemistry,2006,99:244-254. |
| [7] | Kittiphattanabawon P;Benjakul S;Visessanguan W et al.Characterisation of acid-soluble collagen from skin and bone of bigeye snapper(priacanthus tayenus)[J].Food Chemistry,2005,89:363-372. |
| [8] | Nagai T;Suzuki N .Isolation of collagen from fish waste material-skin,bone and fins[J].Food Chemistry,2000,68:277-281. |
| [9] | Nagai T;Worawattanamateekul W;Suzuki N et al.Iso-lation and characterization of collagen from the outer skin waste material of cuttlefish(Sepia lycidas)[J].Food Chemistry,2001,72:425-429. |
| [10] | 汪海波,汪海婴,梁艳萍,张寒俊,刘良忠.草鱼鱼鳞胶原蛋白的凝胶性能研究[J].功能材料,2012(04):433-437,441. |
| [11] | Wang Y;Wang H B;Gui M.Optimization of ex-traction process parameters of collagens from the scale of grass carp[J].Food Science,2010(31):70-76. |
| [12] | CHRISTOPHER A MILES;ALLEN J BAILEY .Thermal denaturation of collagen revisited[J].Proceedings of the Indian Academy of Sciences.Chemical sciences,1999(1):71-80. |
| [13] | Nam K;Kimura T;Kishida A .Preparation and characterization of cross-linked collagen-phospholipid polymer hybrid gels[J].Biomaterials,2007(26):1-8. |
| [14] | Reddy G K;Enwemeka C S .A simplified method for the analysis of hydroxyproline in biological tissue[J].Clini-cal Biochemistry,1996,29:225-229. |
| [15] | Mu CD;Li DF;Lin W;Ding YW;Zhang GH .Temperature induced denaturation of collagen in acidic solution[J].Biopolymers: Original Research on Biomolecules and Biomolecular Assemblies,2007(4):282-287. |
| [16] | Giraud Guille-MM;Besseau L;Chopin C;Durand P;Herbage D .Structural aspects of fish skin collagen which forms ordered arrays via liquid crystalline states.[J].Biomaterials,2000(9):899-906. |
| [17] | Doyle B B;Bendit E G;Blout E R .Infrared spectrosco-py of collagen and collagen-like polypeptides[J].Bio-polymers,1975,14:937-957. |
| [18] | Taravel MN.;Domard A. .COLLAGEN AND ITS INTERACTION WITH CHITOSAN .2. INFLUENCE OF THE PHYSICOCHEMICAL CHARACTERISTICS OF COLLAGEN[J].Biomaterials,1995(11):865-871. |
| [19] | Hickman D.;Sims TJ.;Miles CA.;Bailey AJ.;de Mari M.;Koopmans M. .Isinglass/collagen: denaturation and functionality[J].Journal of Biotechnology,2000(3):245-257. |
| [20] | Chen X L;Yu M X;Chen W S et al.Dynamic changes in dermal cell cycles,contents of hydroproline and ratios of collagen type Ⅰ/Ⅲ in the burned wound[J].Chinese Journal of Surgery,2000,38(02):28-33. |
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