首先对生物大分子γ-聚谷氨酸(γ-PGA)进行自组装,制备γ-PGA自组装胶束粒子,利用 Zeta 电位及纳米粒度分析仪,原子力显微镜(AFM)对胶束粒子的基本性能进行表征.接着以γ-PGA 自组装胶束粒子溶液为电解液,通过电沉积技术,在镁合金表面制备γ-PGA生物纳米涂层材料.采用全反射傅里叶变换红外光谱仪(ATR-FT-IR),X 射线衍射仪(XRD),超景深显微镜,扫描电子显微镜(SEM)对涂层样品进行表征.研究结果表明,γ-PGA 自组装胶束粒子的流体动力学直径约为221．1 nm,所制备的生物纳米涂层材料可降低镁合金的腐蚀速率,且经乙二胺化学交联后涂层的防护性能将得到进一步改善.本文直接使用未改性的γ-PGA 为组装基元制备胶束粒子,为γ-PGA的组装提供了一种简便方法,同时利用电诱导将其固定在金属基材表面制备生物纳米涂层材料,进一步拓宽了基于γ-聚谷氨酸自组装胶束粒子的应用领域.
γ-Polyglutamic acid (γ-PGA)could self-assembly into stable colloidal particles in the selective solvent Dimethyl sulfoxide (DMSO)/Ethanol.The characterization ofγ-PGA colloidal particles were studied by Zeta potential and nano particle size measurements and Atomic force microscope (AFM).Theγ-PGA biomedical nanostructured coating on magnesium alloy was prepared by electrodeposition ofγ-PGA colloidal particles in ethanol.The characterization ofγ-PGA nanostructured coating was conducted by Attenuated Total Reflectance-Fourier Transform Infrared Spectrometer (ATR-FT-IR),Scanning Electron Microscopy (SEM),3D Super Depth Digital Microscope,X-ray Diffraction (XRD),Electrochemical measurement.The results showe that the diameter ofγ-PGA colloidal particles is 221 nm.The corrosion rate of magnesium alloy coated withγ-PGA nanostructured coating is reduced and further chemical cross-linking of the coating by ethylenediamine is also contribution to the corrosion resistance of the substrate.
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