采用二次相分离制备7种 N-组氨酸壳聚糖/聚乳酸(NHCS/PLLA)支架,考察其在模拟体液中对牛血清白蛋白(BSA)的吸附性能,并研究其吸附等温式、吸附动力学和热力学行为等.结果表明,在37．0℃、BSA 的初始浓度为2．5 mg/mL 时,50 kD-NHCS-3与PLLA质量比为5/5制得的 NPs3支架对BSA 的吸附容量最大,达928．53 mg/g；吸附遵循Langmuir吸附等温式和准一级动力学方程,可为分离纯化BSA提供借鉴.
Histidine-grafted-chitosan/poly(L-lactide)(NHCS/PLLA)scaffolds were prepared by the dual phase separation technique.Adsorption behavior of bovine serum albumin (BSA)by NHCS/PLLA scaffolds in simu-lated body fluid (SBF)was investigated,while the adsorption isotherm,adsorption kinetics and thermodynam-ics of behavior were studied too.The results indicated that these scaffolds were effectively to adsorb BSA,NPs3 were the better for adsorbing BSA of all NHCS/PLLA scaffolds.The adsorption capacity of them were 928.53 mg/g,which could be reused for separation and purification of BSA,and could provide a carrier for oth-er protein and tissue engineering.
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