以壳聚糖、咪唑-1-乙酸为原材料,1-乙基-(3-二甲基氨基丙基)碳酰二亚胺盐为引发剂、N-羟基丁二酰亚胺为偶联剂,在2-(N-吗啡啉)乙磺酸(ME S )缓冲体系中将咪唑-1-乙酸接枝到壳聚糖中,冷冻干燥得到具有 pH 值响应性的咪唑-1-乙酸聚糖(IACS).用傅里叶变换红外光谱(FT-IR )、核磁共振(1 H-NMR)、元素分析(EA)、场发射扫描电镜(FESEM)等方法对产物进行表征.结果表明,咪唑-1-乙酸成功接枝到壳聚糖的氨基上；当壳聚糖分子量相同时,随着咪唑-1-乙酸/壳聚糖摩尔比的增加,取代度升高,取代度落在5．52％~7．76％,IACS 支架孔径尺寸分布在50~150μm 之间,呈现三维状结构.当咪唑-1-乙酸壳聚糖溶液 pH 值从4．0增加大6．5时,体系的 Zeta 电位从＋64．2 mV 降低到＋24．2 mV.综上所述,咪唑-1-乙酸壳聚糖支架有望为生物矿化和骨组织生长提供良好的模板.
Chitosan used as target polymer and was modified with imidazole-1-acetic acid.1-(3-dimethylamino propyl)-3-ethyl carbodiimide as initiator and N-hydroxysuccinimide as the coupler,the imidazole-1-acetic acid was grafted to chitosan.A series of imidazole-1-acetic acid modified chitosan (IACS)with different substitution degrees were prepared by using the method of freeze-casting and controlling the amount of Imidazole-1-acetic acid and other conditions.Chemical structure and morphology of IACS were characterized through Fourier transform infrared spectrometer(FT-IR),1 H-NMR spectroscopy,elemental analysis(EA),field emission scan-ning electron microscopy (FESEM)and other methods.The results showed that imidazole-1-acetic acid success-fully grafted onto the amino groups of chitosan,with increasing of the imidazole-1-acetic acid dosage,the degree of substitution increased(5.52%-7.76%),the pore size arranging in 50-150μm,presenting three-dimensional structure.The Zeta value of IACS decreased from +64.2 mV to +24.2 mV with increase of pH value,which proved that IACS has pH responsibility.Based on the above results,it indicted that IACS could provide a good template for biomineralization and be applied in cartilage tissue engineering.
参考文献
| [1] | Park JS;Han TH;Lee KY;Han SS;Hwang JJ;Moon DH;Kim SY;Cho YW .N-acetyl histidine-conjugated glycol chitosan self-assembled nanoparticles for intracytoplasmic delivery of drugs: Endocytosis, exocytosis and drug release[J].Journal of Controlled Release: Official Journal of the Controlled Release Society,2006(1):37-45. |
| [2] | 陈彰旭,辛梅华,李明春.组氨酸改性壳聚糖多孔支架的制备与表征[J].高分子材料科学与工程,2013(09):152-156. |
| [3] | 郑亮,李兆乾,刘勋,裴重华.水溶性壳聚糖/鸡蛋清对仿生碳酸钙晶体形貌的控制[J].人工晶体学报,2012(05):1420-1424. |
| [4] | 张利,李玉宝,杨爱萍,左奕,吕国玉,魏杰.骨组织工程用纳米羟基磷灰石/ 壳聚糖多孔支架材料的制备及性能表征[J].功能材料,2005(02):314-317. |
| [5] | 闵翔,唐敏健,焦延鹏,周长忍.壳聚糖/聚己内酯共混膜的制备及性能[J].中国组织工程研究与临床康复,2011(21):3887-3890. |
| [6] | 李明春,周盛全,辛梅华,宋宇宁,王朝.N-长烷基壳聚糖季铵盐的合成及其抗菌活性研究[J].功能材料,2012(17):2338-2342,2345. |
| [7] | Badawy, M.E.I. .Structure and antimicrobial activity relationship of quaternary n-alkyl chitosan derivatives against some plant pathogens[J].Journal of Applied Polymer Science,2010(2):960-969. |
| [8] | Yang Ziyu .Properties and preparation of N-lactosam-O-histamine acylated carboxymethyl chitosan nanoparticles[D].Wuhan:Wuhan University of Technology,2010. |
| [9] | Magdy W. Sabaa;Nadia A. Mohamed;Riham R. Mohamed .Synthesis, characterization and antimicrobial activity of poly (N-vinyl imidazole) grafted carboxymethyl chitosan[J].Carbohydrate Polymers: Scientific and Technological Aspects of Industrially Important Polysaccharides,2010(4):998-1005. |
| [10] | Fan Bing;Liu Chengsheng;Liu Han et al.Synthesis and antibacterial activity of chitosan derivatives[J].Journal of Functional Materials,2011,42(s3):544-548. |
| [11] | Opanasopit P;Sajomsang W;Ruktanonchai U;Mayen V;Rojanarata T;Ngawhirunpat T .Methylated N-(4-pyridinylmethyl) chitosan as a novel effective safe gene carrier.[J].International Journal of Pharmaceutics,2008(1):127-134. |
| [12] | Mohammad R. Kasaai .Determination of the degree of N-acetylation for chitin and chitosan by various NMR spectroscopy techniques: A review[J].Carbohydrate Polymers: Scientific and Technological Aspects of Industrially Important Polysaccharides,2010(4):801-810. |
| [13] | Ghosn, Bilal;Singh, Ankur;Li, Mu;Vlassov, Alexander V.;Burnett, Chris;Puri, Nitin;Roy, Krishnendu . .Efficient Gene Silencing in Lungs and Liver Using Imidazole-Modified Chitosan As a Nanocarrier for Small Interfering RNA[J].Oligonucleotides,2010(3):163-172. |
| [14] | 程萌萌,辛梅华,李明春,李璇.季铵化-N-（4-咪唑甲基）壳聚糖的制备及其胶束形成[J].化工进展,2012(04):861-865. |
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