针对透明质酸、胶原蛋白水凝胶力学性能差的问题,采用两步交联法将透明质酸、胶原蛋白与聚乙二醇双丙烯酸酯制备成一种兼具生物相容性和高强度的复合水凝胶.研究制备了具有相同胶原蛋白和聚乙二醇双丙烯酸酯质量分数,不同透明质酸质量分数的水凝胶,从微观结构和力学强度调控了复合水凝胶的理化性能,并用 L-赖氨酸对复合凝胶进行改性.SEM结果表明,三元复合水凝胶具有蜂窝状的内部结构,孔径范围在80~180μm,赖氨酸浓度达40 mmol/L时,水凝胶的压缩模量达414 kPa,是改性前模量的19倍.细胞毒性和体内植入实验表明该水凝胶浸提环境对细胞生长无明显抑制作用,产生较低的免疫排斥反应,有望用于软骨等组织的修复.
Aiming at the brittleness of hyaluronic acid (HA)and collagen (Col)hydrogels in mechanical proper-ties,this work was to prepare a composite hydrogel with biocompatibility and high strength by two-step cross-linking method.Hydrogels with different weight percent of HA and constant Col and poly (ethylene glycol)Di-acrylate were obtained to tune the physical and chemical properties.Subsequently,L-lysine was used to modify the hydrogels.The SEM results show the honeycomb morphology and the pore size of the ternary hydrogel ran-ges from 80-180 μm.When the concentration of L-lysine is 40 mmol/L,the comperssion modulus reaches 414 kPa which is 1 9 times of non-modified hydrogels.Cell toxicity and implantation experiments indicate that the extracts of hydrogel has no obvious inhibitory effect on MC 3T3-E1 cells,and the slight immunological re-jection on mice.This kind of composite hydrogel has great potential in cartilage repair.
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