材料期刊网

采用水刺法制备了多孔丝素蛋白(SF)支架,通过原位矿化法制备了纳米羟基磷灰石(HAP)/丝素蛋白复合材料.HAP 呈现均匀棒状结构,而没有丝素蛋白支架时 HAP 仅形成颗粒状结构,这表明蛋白支架可诱导 HAP 形成均匀棒状结构,这可能是源于丝素蛋白中羧基和溶液中 Ca2＋的静电作用；丝素蛋白支架起到了复合材料基材和 HAP 原位生长的高分子模板双重作用.采用红外光谱、X 射线衍射和力学性能测试对复合材料的结构性能进行了表征,红外谱图表明,HAP 中的 PO 3－4与 SF 中的—NH 2存在强烈的氢键相互作用,而 X 射线衍射图谱则表明复合材料中 SF 的保持了β折叠结构和结晶性能,使得复合材料具有良好的力学性能和多孔性能,有利于成骨细胞在支架上的增殖,同时,该方法制备的复合材料力学性能好,可大面积制备,成本低、制备方便,易于实现工业化,可望广泛用于骨修复支架材料.

Porous silk fibroin scaffold was first prepared by using spunlacing technique,then nano-hydroxyapa-tite (HAP)/silk fibroin composite materials were synthesized via in-situ mineralization.HAP showed a uniform rod-like structure,but only granular structures were observed without silk fibroin scaffold,indicating that silk fibroin scaffold can induce the formation of rod-like structures.This was attributed to the electrostatic interac-tions between carboxyl groups of silk fibroin and Ca2 + in the solution.Thus silk fibroin scaffold plays a dual role of polymer composites substrate and template for HAP in-situ growth.The structure and properties of the com-posites were characterized by using infrared spectroscopy,X-ray diffraction and mechanical testing.Infrared spectra showed the presence of hydrogen bonding interactions between PO 3 -4 of HAP and —NH 2 of silk fibroin, while X-ray diffraction patterns suggested that the composites maintained the β-sheet structure and crystalliza-tion properties of silk fibroin scaffold.Thus the composites resulted with good mechanical properties and porous structure,which was helpful in osteoblast proliferation and the use as bone repair scaffolds.At the same time, the composite materials showed very well mechanical performance,but easy preparation for large area,low cost,easy to realize industrialization,and will be possible used for bone repair scaffold material widely in fu-ture.