在模拟体内pH值=7.4的胶原溶胶-凝胶体系中,加入β-甘油磷酸钠(β-GP)作为矿化的磷源,并引入聚丙烯酸(PAA)和三聚磷酸钠(TPP)作为“软模板”模拟体内非胶原蛋白DMP-1的N端和C端的隔离功能域,调控工型胶原的矿化,构建了纳米羟基磷灰石/胶原(n-HA/COL)复合支架材料.并以人脐带间充质干细胞hUMSCs作为种子细胞,探究了其对细胞的粘附、增殖、分化的影响.结果表明,胶原纤维组织与矿化同步进行,陈化处理有利于羟基磷灰石(HAp)晶体的结晶.自组装HAp纳米微粒不仅在胶原纤维外部沉积,也可以在原胶原分子上及胶原微纤维的末端被发现,同时纳米磷酸钙盐沿着胶原微纤维的圆柱形表面分布.细胞培养表明n-HA/COL并没有明显促进hUMSCs细胞的增殖,但能够诱导hUMSCs向成骨细胞分化,促进成骨细胞AKP的表达,并且n-HA/COL复合支架材料与成骨诱导液(OICM)联用时诱导效果更加显著.这种以“bottom-up”深度矿化方法为骨支架材料的制备提供了新思路,n-HAp/collagen复合材料有望用于骨组织填充修复.
参考文献
| [1] | Sang-Hoon Rhee;Jae Do Lee.Nucleation of Hydroxyapatite Crystal through Chemical Interaction with Collagen[J].Journal of the American Ceramic Society,200011(11):2890-2892. |
| [2] | Crane NJ;Popescu V;Morris MD;Steenhuis P;Ignelzi MA Jr.Raman spectroscopic evidence for octacalcium phosphate and other transient mineral species deposited during intramembranous mineralization.[J].Bone,20063(3):434-442. |
| [3] | Tsuji, T;Onuma, K;Yamamoto, A;Iijima, M;Shiba, K.Direct transformation from amorphous to crystalline calcium phosphate facilitated by motif-programmed artificial proteins[J].Proceedings of the National Academy of Sciences of the United States of America,200844(44):16866-16870. |
| [4] | Wang, LJ;Nancollas, GH.Pathways to biomineralization and biodemineralization of calcium phosphates: the thermodynamic and kinetic controls[J].Dalton transactions: An international journal of inorganic chemistry,200915(15):2665-2672. |
| [5] | Hao J;Ramachandran A;George A.Temporal and spatial localization of the dentin matrix proteins during dentin biomineralization.[J].The Journal of Histochemistry and Cytochemistry,20093(3):227-237. |
| [6] | Zhao L;Burguera EF;Xu H.Fatigue and human umbilical cord stem cell seeding characteristics of calcium phosphate-chitosan-biodegradable fiber scaffolds.[J].Biomaterials,20105(5):840-847. |
| [7] | 沈小兵;李波;赵军;许为中;孙维均.水热法调控羟基磷灰石微结构研究进展[J].功能材料,2014(3):03006-03010. |
| [8] | 丁珊;唐敏健;周长忍;田金环;李立华.仿生功能化壳聚糖的合成及矿化行为研究[J].功能材料,2012(02):174-177. |
| [9] | Liu Y;Kim YK;Dai L;Li N;Khan SO;Pashley DH;Tay FR.Hierarchical and non-hierarchical mineralisation of collagen.[J].Biomaterials,20115(5):1291-1300. |
| [10] | Kim YK;Gu LS;Bryan TE;Kim JR;Chen L;Liu Y;Yoon JC;Breschi L;Pashley DH;Tay FR.Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein analogues in the presence of calcium, phosphate and hydroxyl ions.[J].Biomaterials,201025(25):6618-6627. |
| [11] | Anton Ficai;Ecaterina Andronescu;Georgeta Voicu.Self-assembled collagen/hydroxyapatite composite materials[J].Chemical engineering journal,20102(2):794-800. |
| [12] | Xu, Z.;Neoh, K.G.;Kishen, A..A biomimetic strategy to form calcium phosphate crystals on type i collagen substrate[J].Materials science & engineering, C. Biomimetic and supramolecular systems,20106(6):822-826. |
| [13] | Yuping Li;Amran Asadi;Margo R. Monroe;Elliot P. Douglas.pH effects on collagen fibrillogenesis in vitro: Electrostatic interactions andphosphate binding[J].Materials science & engineering, C. Biomimetic and supramolecular systems,20095(5):1643-1649. |
| [14] | Yang, X. J.;Liang, C. Y.;Cai, Y. L.;Hu, K.;Wei, Q.;Cui, Z. D..Recombinant human-like collagen modulated the growth of nano-hydroxyapatite on NiTi alloy[J].Materials science & engineering, C. Biomimetic and supramolecular systems,20091(1):25-28. |
| [15] | Zhai Y;Cui FZ.Recombinant human-like collagen directed growth of hydroxyapatite nanocrystals[J].Journal of Crystal Growth,20061(1):202-206. |
| [16] | Kikuchi M;Itoh S;Ichinose S;Shinomiya K;Tanaka J.Self-organization mechanism in a bone-like hydroxyapatite/collagen nanocomposite synthesized in vitro and its biological reaction in vivo.[J].Biomaterials,200113(13):1705-1711. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%