采用新型交联剂氧化低聚异麦芽糖(IMODA)交联脱细胞猪真皮基质(pADM)，优化最佳交联条件，考察了交联材料(IMODA-pADM)的收缩温度、改性指数、傅里叶红外光谱、机械性能、亲水性、吸湿性、表面形貌、耐酶解性能及细胞相容性.结果显示，交联材料的收缩温度随 IMODA 的用量、反应温度、反应时间的增加而先增大后趋于稳定，随pH 值的增加而先增大后减小.交联最优条件为，反应温度37℃、用量16％、反应时间18 h、pH 值为9．4；随用量增大，交联材料改性指数增大，物理机械性能提高，亲水性与吸湿性略有降低，耐酶解性能大幅提高；交联后材料更为紧实，材料表面有利于细胞粘附生长，细胞形态良好，细胞毒性为0-Ⅰ级.
Porcine acellular dermal matrix (pADM)was crosslinked by a new type crosslinker which is called isomaltooligosaccharide dialdehyde (IMODA),the crosslinking conditions were optimized.the modification in-dex,fourier transform infrared spectroscopy,mechanical properties,hydrophilicity,scanning electron micros-copy and the cytotoxicity of IMODA-pADM were tested.The results indicated that when the dosage,reaction temperature and reaction time were increased,the shrinkage temperature was increased firstly and then gradual-ly stayed steady,when the pH value was increased,the shrinkage temperature was increased firstly and then de-creased.It was showed that the comprehensive performance was best when the reaction temperature was 37 ℃, the reaction time was 48 h,dosage was 16%,pH value was 9.4.After crosslinking,the tensile strength of IMO-DA-pADM was improved,the hydrophilicity was weakened,the surface of the material is conducive to the ad-hesion and growth of cells,and the ctotoxicity grade of crosslinked material was grade 0-Ⅰ.
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