采用 W/O/W复乳界面聚合法以戊二醛作为交联剂,制备盐酸青藤碱聚乙烯醇(SM-PVA)微胶囊.扫描电镜和傅里叶红外光谱分析戊二醛用量对SM-PVA微胶囊结构的影响,动态膜透析法研究微胶囊体外药物释放行为,MTT法评价微胶囊体外细胞毒性.结果表明 SM-PVA 微胶囊表面光滑、分散性良好；随着戊二醛用量增加,微胶囊囊壁增厚,结构紧实；体外药物释放结果显示戊二醛用量及释放介质 pH 值对药物释放均有影响；且体外细胞毒性结果表明微胶囊对 L929细胞无细胞毒性.SM-PVA 微胶囊具有缓释性能和良好的生物相容性,可进一步研制成为新型中药给药制剂.
Polyvinyl alcohol (PVA)microcapsules carrying Sinomenine hydrochloride (SM)were prepared by W/O/W double emulsion interfacial polymerization technique with glutaraldehyde as a cross linker.The effects of content of cross linker on structure of SM-PVA microcapsules were studied by SEM and FT-IR.In vitro re-lease and cytotoxicity were studied by dynamic membrane dialysis and MTT method respectively.The results showed that SM-PVA microcapsules were approximate to spheres,smooth with almost homogeneous structure. The thickness of capsule wall was increased with increasing the content of glutaraldehyde.The in vitro release results demonstrated glutaraldehyde dosage and pH value had a significant effect on drug release.The cell ex-periments showed that SM-PVA microcapsules have no cytotoxicity to L929 cell.The research indicates that SM-PVA microcapsules have good controlled release characteristics and biological activity,which can be used as a potential delivery system.
参考文献
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%