在高分子膜表面通过化学方法构建糖基化层，实现对生物膜致密“糖被“层的仿生模拟，可以充分拓展及发挥膜材料和糖的生物学功能.利用二乙烯基砜与羟基反应的特性，以乙烯基砜为偶联剂将甘露糖接枝到聚甲基丙烯酸-2-羟乙酯(PHEMA)聚合物膜表面，制备甘露糖糖基化 PHEMA膜.利用傅里叶变换红外光谱对糖基化膜结构进行了表征，根据血凝素蛋白特异性识别糖的性质对糖基化反应条件进行了优化，并在蛋白和细胞水平对其进行了生物学评价.结果表明，制备的甘露糖糖基化 PHEMA 膜具有良好的抗垢性能，能够特异性识别和吸附伴刀豆球蛋白A(ConA)；糖基化膜能够引起小鼠单核巨噬细胞(RAW 264．7)特异性粘附且具有较低的细胞毒性.提供了一种高分子膜表面糖基化的简单方法，为仿生膜表面的构建以及细胞表面糖参与的生物代谢活动的研究提供了基础.
Glycosylation of polymer film is essential for mimicking the dense “glycocalyx”in biomembrane and development of bio-functional materials.In this study,a convenient method was developed to conj ugate glycose onto poly(2-hydroxyethyl methacrylate)(pHEMA)film using divinyl sulfone(DVS)as the coupling agent.A mannose glycosylated pHEMA film was prepared and characterized by Fourier transform infrared spectroscopy (FT-IR).The reaction conditions were optimized using specific protein adsorption studies,and the bioactivity of the glycosylated film was assessed at protein and cell level.The protein level results show that the mannose gly-cosylated pHEMA film has good anti-fouling capabilities and can specifically recognize Concanavalin A (ConA). The cell adhesion experiment using macrophages (RAW 264.7)suggest that the glycosylated film is highly bio-active and low cytotoxic.The glycosylation of polymer film using DVS provides a neat approach for the con-struction of biomimetic membrane and researches on glycometabolism process at cell level.
参考文献
| [1] | Zeng, X.;Andrade, C.A.S.;Oliveira, M.D.L.;Sun, X.-L..Carbohydrate-protein interactions and their biosensing applications[J].Analytical and bioanalytical chemistry,201210(10):3161-3176. |
| [2] | Halina Lis;Nathan Sharon.Lectins: Carbohydrate-Specific Proteins That Mediate Cellular Recognition[J].Chemical Reviews,19982(2):637-674. |
| [3] | Christiansen,M.N.;Chik,J.;Lee,L.;Anugraham,M.;Abrahams,J.L.;Packer,N.H..Cell surface protein glycosylation in cancer[J].Proteomics,20144/5(4/5):525-546. |
| [4] | Wang, C.;Wu, J.;Xu, Z.-K..High-density glycosylation of polymer membrane surfaces by click chemistry for carbohydrate-protein recognition[J].Macromolecular rapid communications: Publishing the newsletters of the European Polymer Federation,201012(12):1078-1082. |
| [5] | Revell DJ.;Blyth DJ.;Haines AH.;Russell DA.;Knight JR..Self-assembled carbohydrate monolayers: Formation and surface selective molecular recognition[J].Langmuir: The ACS Journal of Surfaces and Colloids,199816(16):4517-4524. |
| [6] | Spiro RG.Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds.[J].Glycobiology,20024(4):43R-56R. |
| [7] | Mathias Ulbricht.Advanced functional polymer membranes[J].Polymer: The International Journal for the Science and Technology of Polymers,20067(7):2217-2262. |
| [8] | Ladmiral V.;Melia E.;Haddleton DM..Synthetic glycopolymers: an overview [Review][J].European Polymer Journal,20043(3):431-449. |
| [9] | Yang Q;Hu MX;Dai ZW;Tian J;Xu ZK.Fabrication of glycosylated surface on polymer membrane by UV-induced graft polymerization for lectin recognition[J].Langmuir: The ACS Journal of Surfaces and Colloids,200622(22):9345-9349. |
| [10] | Fang, Y;Huang, XJ;Chen, PC;Xu, ZK.Polymer materials for enzyme immobilization and their application in bioreactors[J].BMB Reports,20112(2):87-95. |
| [11] | Shizuka Egusa;Shingo Yokota;Kyoko Tanaka.Surface modification of a solid-state cellulose matrix with lactose by a surfactant-enveloped enzyme in a nonaqueous medium[J].Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology,200913(13):1836-1842. |
| [12] | Wang, Hanqi;Cheng, Fang;Li, Mingyang;Peng, Wei;Qu, Jingping.Reactivity and Kinetics of Vinyl Sulfone-Functionalized Self-Assembled Monolayers for Bioactive Ligand Immobilization[J].Langmuir: The ACS Journal of Surfaces and Colloids,201511(11):3413-3421. |
| [13] | Yu Yu;Ying Chau.One-Step "Click" Method for Generating Vinyl Sulfone Groups on Hydroxyl-Containing Water-Soluble Polymers[J].Biomacromolecules,20123(3):937-942. |
| [14] | Youmie Park;Kai Y. Kwok;Chawki Boukarim;Kevin G. Rice.Synthesis of Sulfhydryl Cross-Linking Poly(Ethylene Glycol)-Peptides and Glycopeptides as Carriers for Gene Delivery[J].Bioconjugate Chemistry,20022(2):232-239. |
| [15] | Shang, J.;Cheng, F.;Dubey, M.;Kaplan, J.M.;Rawal, M.;Jiang, X.;Newburg, D.S.;Sullivan, P.A.;Andrade, R.B.;Ratner, D.M..An organophosphonate strategy for functionalizing silicon photonic biosensors[J].Langmuir: The ACS Journal of Surfaces and Colloids,20126(6):3338-3344. |
| [16] | Monireh Bakhshpour;Nilay Bereli;Serap ?enel.Preparation and characterization of thiophilic cryogels with 2-mercapto ethanol as the ligand for IgG purification[J].Colloids and Surfaces, B. Biointerfaces,2014:261-268. |
| [17] | Kou RQ.;Xu ZK.;Deng HT.;Liu ZM.;Seta P.;Xu YY..Surface modification of microporous polypropylene membranes by plasma-induced graft polymerization of alpha-allyl glucoside[J].Langmuir: The ACS Journal of Surfaces and Colloids,200317(17):6869-6875. |
| [18] | Hileman RE;Fromm JR;Weiler JM;Linhardt RJ.Glycosaminoglycan-protein interactions: definition of consensus sites in glycosaminoglycan binding proteins.[J].BioEssays: news and reviews in molecular, cellular and developmental biology,19982(2):156-167. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%