弹性蛋白有良好的弹性、稳定性和生物相容性，可作为构建组织工程复合材料的组成部分。本文以4种不同的酚类物质为介体，通过酪氨酸酶催化氧化介体从而对弹性蛋白进行酶法交联，并借助静电纺丝方法制备纳米纤维膜。结果表明，酪氨酸酶能催化氧化酚类介体物质，且酶催化效率与酚类物质的种类有关；SDS-PAGE凝胶电泳和体积排阻色谱(SEC)表明弹性蛋白在酪氨酸酶/咖啡酸以及酪氨酸酶/儿茶素体系中发生了交联反应，生成了大分子蛋白聚合物。圆二色谱(CD)结果表明酶促交联反应导致弹性蛋白二级结构发生变化，α-螺旋结构含量增多。酪氨酸酶/儿茶素催化体系改善了弹性蛋白的可纺性，制得的纳米纤维粗细均匀，且膜材料有较好的生物相容性。
Elastin has excellent properties of elasticity,stability and biocompatibility,and it could be used as a maj or composite biomaterial for tissue engineering.In the present work,the enzyme-catalyzed cross-linking of elastin was carried out by using tyrosinase and four mediators of phenolic compounds,followed by preparation of elastin nano-fiber membrane by using electrospinning method.The results indicated that tyrosinase could oxi-dize the phenolic mediators and the oxidation efficiency was highly depended on the sort of phenolic compounds. SDS-PAGE and size exclusion chromatography (SEC)analysis demonstrated that elastin could be cross-linked by tyrosinase in the presence of catechin or caffeic acid,resulting in an increase of molecular weight of elastin. Circular Dichroism (CD)data revealed that the enzymatic cross-linking led to the changes of the secondary structures,the content ofα-Helix was increased.Modification of elastin with tyrosinase/catechin improved elas-tin’s spinnability,and the obtained nano-fibers exhibited uniform fineness and good biocompatibility as well.
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