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以天然鱼源胶原为基质材料,以罗丹明B为模拟负载目标药物,采用不同方法制备负载罗丹明B的胶原缓释材料,以负载率和材料缓释性能作为评价指标,优化了胶原基生物缓释材料的制备工艺条件,探讨了胶原负载罗丹明B的缓释机制.实验结果表明,在直接共混、共混/组装、共混/组装/离心及共混/组装/离心/交联几种方法中,前2种方法制备材料对罗丹明B的负载率较高,但负载材料的缓释效果较差;而后2种方法罗丹明B负载率虽然较低,但展现出较好的缓释能力,其中共混/组装/离心方法的缓释效果最佳.在此基础上,进一步探讨了材料干燥方法和胶原浓度对材料缓释效果的影响.结果表明,在-22,-64,-196 ℃预冻后冷冻干燥及30 ℃烘箱干燥几种方法中,-64 ℃预冻后冷冻干燥形成的材料缓释效果最佳;提高胶原浓度后,材料的负载和缓释能力均有显著提升.针对胶原基负载罗丹明B材料的缓释动力学研究表明,该材料对罗丹明B的释放机理是基于菲克定律的Higuchi动力学模型的扩散过程;红外光谱分析进一步表明胶原对罗丹明B的吸附同时包含物理吸附和化学吸附两种形式;材料的电镜观察结果证实,该材料中罗丹明B大部分镶嵌或包裹在胶原纤维中,只有少部分被吸附在纤维表面.

Using fish collagen as load matrix materials, rhodamine B as a simulated load target drug, collagen sustained release material was prepared by different methods.The preparation conditions of collagen biomaterials were optimized according the load rate and release properties of rhodamine B and the release mechanism of collagen loading rhodamine B was discussed.The results showed that in several load methods (direct blend, blend + self-assembly, blend + self-assemble + centrifuge, blend + self-assemble + centrifuge + cross-linking) , the first two methods have higher loading efficiency to target substance, but bad release ability.Although the load rate of latter two methods is not high, the materials have sustained release capability and the method of blend + self-assemble + centrifuge shows the best sustained release effect.Based on that, the effects of the drying method and collagen concentration of material on the sustained release ability were further studied.The results showed that the freeze-dried material pre-frozen under the temperature of-64 ℃ has the best sustained release effect among different drying methods (freeze-dried after pre-frozen at-22,-64,-196 ℃ and drying at room temperature of 30 ℃).Increasing collagen concentration can improve the materials' capabilities of loading and sustained release to rhodamine B.The study results of sustained release kinetics showed that the release mechanism of collagen based material loading rhodamine B is based on the fick's law Higuchi kinetic model of diffusion process.IR analysis showed that the collagen adsorpting rhodamine B includes two forms, physical and chemical adsorption.Morphological analysis showed that there are small parts of rhodamine B adsorption on the fiber surface and most of them were packaged in the collagen fibers gaps.