利用广泛使用且具有良好生物相容性的壳聚糖为基材,采用生物交联剂京尼平交联壳聚糖,制备具有不同力学性能的壳聚糖材料.探讨不同实验条件对于材料性能的影响及材料力学性能与细胞行为之间的关系.结果表明,投料比、反应温度、反应时间均能在不同程度上影响材料的交联程度及各项性能,其中投料比对于膜材料的性能影响最为明显.通过在不同力学性能的二维材料上培养前成骨细胞MC3T3-E1发现,随着材料力学强度的增高,材料上的细胞增殖率不断增大,但到了一定限度,增殖率开始减小;而细胞的成骨分化能力则一直增大.
In this study, chitosan was used as primary materials for its good biocompatibility and a series of cross linked chitosan membrane which have different mechanical properties were prepared by the biological crosslinking agent genipin.The influence of the different experimental conditions on the mechanical properties of the crosslinked membrane and the relationship between the mechanical properties of the membrane and the behavior of the cells were investigated.The results show that the properties of the crosslinked membrane can be affected by the amount of crosslinking agent, reaction temperature and reaction time in different degree, among which the amounts of crosslinking agent affect most obviously.The proliferation and differentiation of pre-osteoblast MC3T3-E1 cultured on two-dimensional membrane materials surface which with different mechanical properties have indicated that the cell proliferation rate increased with the increasing mechanical strength of materials, but decreased when the membrane mechanical strength was too high.However, the osteogenic differentiation ability increased with the mechanical strength increasing.
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