设计合成了均苯三甲酸单缩N-羟基琥珀酰亚胺单缩乙二醇酯双功能螯合剂(TEN)及其与稀土离子形成的配合物,通过元素分析、稀土滴定确定了配合物的组成为:RE(TEN)2·[OH]·mH2O·nNH3,[RE=Eu3+,Tb3+;TEN=C15H13O9N].配合物的红外光谱表明,配体TEN中的一个羧基与稀土离子配位.荧光光谱实验表明,两种稀土配合物的荧光强度较高,有明显的特征荧光.配合物中的N-羟基琥珀酰亚胺具有与生物分子连接的能力,因此合成的配合物在生物标记功能材料方面具有潜在的应用前景.
参考文献
| [1] | Mingqian Tan;Zhiqiang Ye;Guilan Wang;Jingli Yuan .Preparation and Time-Resolved Fluorometric Application of Luminescent Europium Nanoparticles[J].Chemistry of Materials,2004(12):2494-2498. |
| [2] | Feng-Bo W;Shi -Quan H;Chao Z et al.Synthesis of a highly fluorescent β-diketone-europium chelate and its utility in time-resolved fluoroimmunoassay of serum total thyroxine[J].Analytical Chemistry,2002,74(22):5882-5889. |
| [3] | Soini E;Lovgren T .Time-resolved fluorescence of lanthanide probes and applications in biotechnology[J].Critical Reviews in Analytical Chemistry,1987,18:105-154. |
| [4] | Diamandis E P;Christopoulos T K .Europium chelate labels in time-resolved fluorescence immunoassay and DNA hybridization assays[J].Analytical Chemistry,1990,62(22):1149A-1157A. |
| [5] | Yuan JL.;Kimura H.;Matsumoto K. .A new tetradentate beta-diketonate-Europium chelate that can be covalently bound to proteins for time-resolved fluoroimmunoasaay[J].Analytical chemistry,1998(3):596-601. |
| [6] | Yuan J;Wang G;Kimura H .Highly sensitive time-re-solved flu-oroimmunoassay of human immunoglobulin E by using a new europium fluoresent chelate as label[J].Analytical Biochemistry,1997,254(02):283-287. |
| [7] | Wu F;Zhang C .A new diketon-europium chelate for ultrasensitive time-resolved fluorescence immunoassays[J].Analytical Chemistry,2002,311(01):57-67. |
| [8] | Wu F;Han S Q;Zhang C .Sythnesis of a highly fluorescent betadiketon-europium chelate and its utility in time -resolved fluoroimmuno-assay of serum total thyroxine[J].Analytical Chemistry,2002,72(22):5882-5889. |
| [9] | 潘利华,周誓红,孙文伟,谢文兵,赵超.固相时间分辨荧光免疫标记技术研究[J].光谱学与光谱分析,2004(12):1601-1604. |
| [10] | 赵劼,马庆杰,赵伟,潘立华,徐文贵,孙文伟.时间分辨荧光免疫分析螯合剂的制备及标记方法[J].吉林大学学报(医学版),2003(06):810-812. |
| [11] | 江祖成;蔡汝秀;张华山.稀土元素分析化学[M].北京:科学出版社,2000:224-232. |
| [12] | Sato S;Wada M .Relations between intramoleclar energy transfer efficiencies and tripite state energies in rare earth diketone chelates[J].Bulletin of the Chemical Society of Japan,1970,43(07):1955-1962. |
| [13] | Dexter D L .A theory of sensitized luminescence in solids[J].Journal of Chemical Physics,1953,21(05):836-841. |
| [14] | Brown T D;Shepherd T M.Factors affecting the quanturn efficiencies of fluorescent terbium(Ⅲ)chelates in solid state[J].Journal of the Chemical Society,1973(03):336-341. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%