金属与非金属纳米颗粒增强葡萄糖生物传感器

材料科学与工艺, 2004, 12(2): 163-166. doi: 10.3969/j.issn.1005-0299.2004.02.014

金属与非金属纳米颗粒增强葡萄糖生物传感器

孟宪伟 ^1, , 冉均国 ^2, , 苟立 ^3, , 苏葆辉 ^4, , 王方瑚 ^5, , 段莉 ^6, , 唐芳琼 ^7,

1.四川大学,无机材料系,四川,成都,610065;中国科学院,理化技术研究所,北京,100101

2.四川大学,无机材料系,四川,成都,610065

3.四川大学,无机材料系,四川,成都,610065

4.四川大学,无机材料系,四川,成都,610065

5.四川大学,无机材料系,四川,成都,610065

6.四川大学,无机材料系,四川,成都,610065

7.中国科学院,理化技术研究所,北京,100101

基金项目: 国家自然科学基金(20301015) 高等学校博士学科点专项科研项目(69772039)

关键词：纳米颗粒 , 固定化酶 , GOD , 灵敏度

Enhancement effect of metal and nonmetal nanoparticles on glucose biosensor sensitivity

为了提高葡萄糖传感器的灵敏度和抗干扰性,利用纳米增强效应,以Au、Ag、Pt、SiO2纳米颗粒及金属-无机复合纳米颗粒与聚乙烯醇缩丁醛(PVB)构成复合固定酶膜基质,采用溶胶-凝胶法固定葡萄糖氧化酶(GOD),组成葡萄糖生物传感器.研究表明,纳米颗粒可以大幅度地提高固定化酶的催化活性,增加电极的电流响应灵敏度,改进生物传感器的抗干扰性能,使信噪比提高了32倍.

参考文献

[1]	Zhou DM.;Chen HY.;Ju HX. .A MINIATURIZED GLUCOSE BIOSENSOR BASED ON THE COIMMOBILIZATION OF GLUCOSE OXIDASE AND FERROCENE PERCHLORATE IN NAFION AT A MICRODISK PLATINUM ELECTRODE[J].Sensors and Actuators, B. Chemical,1997(2/3):89-94.
[2]	Cass A E G;FRANCIS D G;HAO H .Ferrocene-mediated enzyme electrode for amperometric determination of glucose[J].Analytical Chemistry,1984,56:667-671.
[3]	Mukhopadhyay K.;Phadtare S.;Vinod VP.;Kumar A.;Rao M.;Chaudhari RV. Sastry M. .Gold nanoparticles assembled on amine-functionalized Na-Y zeolite: A biocompatible surface for enzyme immobilization[J].Langmuir: The ACS Journal of Surfaces and Colloids,2003(9):3858-3863.
[4]	Francesca Sbrana;Maria Teresa Parodi;Davide Ricci;Ermanno Di Zitti .Langmuir films of thiolated gold nanoparticles transferred onto functionalized substrate: 2-D local organization[J].Materials science & engineering, C. Biomimetic and supramolecular systems,2002(2):187-191.
[5]	Zhao J;HENKENS R W;STONEHUERNER J et al.Direct electron transfer at horseradish peroxidase-colloidal gold modified electrodes[J].Journal of Electroanalytical Chemistry,1992,327:109-119.
[6]	TANG Fangqiong,CHEN Dong,MENG Xianwei,RAN Junguo,ZHENG Changqiong.Glucose biosensor enhanced by nanoparticles[J].中国科学B辑（英文版）,2000(03):268-274.
[7]	孟宪伟,唐芳琼,冉均国,苟立.纳米颗粒复合材料增强的葡萄糖生物传感器[J].化学通报,2001(06):365-367,364.
[8]	KULYS J J.Enzyme electrodes based on organic metals[J].Biosensors,1986(02):3-11.
[9]	唐芳琼,韦正,陈东,孟宪伟,苟立,冉均国.亲水金和憎水二氧化硅纳米颗粒对葡萄糖生物传感器响应灵敏度的增强作用[J].高等学校化学学报,2000(01):91-94.
[10]	Bartlett P N;WHITAKER R G .Strategies for the development of amperometric enzyme electrodes[J].Biosensors,1987,3:359-379.
[11]	Hecht H J;KALISZ H M;HENDLE J et al.Crystal structure of glucose oxidase from aspergillus niger refined at 2.3 A resolution[J].Journal of Molecular Biology,1993,229:153-172.

上一张下一张

计量

下载量()
访问量()

作者相关

文章评分

您的评分：
1

0%
2

0%
3

0%
4

0%
5

0%

材料期刊网

金属与非金属纳米颗粒增强葡萄糖生物传感器

Enhancement effect of metal and nonmetal nanoparticles on glucose biosensor sensitivity

参考文献