环境因素对硫酸盐还原菌生长的影响

中国腐蚀与防护学报, 2000, 20(4): 224-229. doi: 10.3969/j.issn.1005-4537.2000.04.006

环境因素对硫酸盐还原菌生长的影响

张小里 ^1, , 陈志昕 ^2, , 刘海洪 ^3, , 郭生武 ^4, , 陈开勋 ^5,

1.西北大学化工系西安 710069

2.中国天然气总公司管材所西安 710065

3.西北大学化工系西安 710069

4.中国天然气总公司管材所西安 710065

5.西北大学化工系西安 710069

关键词：硫酸盐还原菌(SRB)细胞培养细菌腐蚀油田注水

EFFECT OF ENVIRONMENT FACTORS ON THE GROWTH OF SULFATE-REDUCING BACTERIA

Keywords： sulfate-reducing bacteria(SRB)

研究了油田注水井中分离的硫酸盐还原菌(SRB)的生长特性．结果表明：SRB菌株不是严格的厌氧菌，它能耐受4.5mg／L浓度的溶解氧，但在9.0 mg／L的高溶解氧浓度下不能生长．NaCl浓度小于0.818％时SRB可正常生长，在0.972％～2.28％时只能在水下沉积物中生长，大于2.45％时生长完全受到抑制．铁离子浓度增大，SRB代谢活力增强，生长高峰期延长，Fe2+限制SRB生长的浓度下限为小于6.8mg/L高Fe2+浓度对SRB生长无抑制作用．厌氧环境下SRB生长的适宜pH为6.5～7.5，pH小于5.5或大于8.0时SRB不能生长；有氧环境下SRB在pH8～8.5时仍能生存乃至增殖．

D. dlesulfuricans Separated from oil flooding water were studied. The results showed that the SRB strain can resist the dissolved oxygen concentration of 4.5 mg/L, but could not grow at the dissolved oxygen concentration of 9.0 rng/L. It was not obligate anaerobes but annex anaerobes. It could grow both in liquid and deposit at the NaCl concentration below 0. 818 %, and could grow only deposit in the NaCl concentation range of 0. 972 % to 2.28 %, but could not grow when NaCl concentration was over2.45 % .Increasing Fe2+ concentration was better for the SRB growing, and SRB could not grow when Fe2+ concentration was below 6.8 rng/L. High Fe2+ concentration has no repressing effect on SRB. In anoxic condition the appropriate pH range for growing was between 6.5～7.5 , and SRB could not grow when pH<5.5 or pH>8.0. In oxygenic condition the SRB could also grow between pH 8.0～8.5.

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