应用分子自组装技术,在SiO2表面衍生活泼醛基,在SiO2表面有125 nm的衍生物,衍生的醛基和氨基发生共价反应而将人免疫球蛋白G固定在二氧化硅表面,抗原抗体反应显示固定的抗体有活性.应用微加工技术加工含交叉排列的椭圆形微柱阵列的微流控芯片,有效增加内表面积和流体接触机会,用同样修饰方法修饰微流控芯片内表面并固定人免疫球蛋白G,流经管道的相应抗抗体和其发生反应而被吸附在管道表面,实现对该抗抗体的亲和富集,富集后荧光密度增加15倍.表面修饰技术能实现蛋白质在二氧化硅表面的固定并保持其生物活性,结合微流控芯片能实现对相应蛋白质的微量富集.
参考文献
| [1] | Z.L. Zhang;C. Crozatier;M. Le Berre;Y. Chen .In situ bio-functionalization and cell adhesion in microfluidic devices[J].Microelectronic engineering,2005(3):556-562. |
| [2] | Uchiyama K;Yang M;Sawazaki T;Shimizu H;Ito S .Development of a Bio-MEMS for Evaluation of dioxin toxicity by immunoassay method[J].Sensors and Actuators, B. Chemical,2004(1/2):200-205. |
| [3] | Zangar R C;Varnum S M;Bollinger N .Studying cellular processes and detecting disease with protein microarrays[J].Drug Metabolism Reviews,2005,37(03):473-487. |
| [4] | Bhalerao K D;Mwenifumbo S C;Soboyejo A B et al.Bounds in the sensitivity of BioMEMS devices for cell detection[J].Biomedical Microdevices,2004,6(01):23-31. |
| [5] | Hoyer Hansen-G;Hamers MJ;Pedersen AN;Nielsen HJ;Brunner N;Dano K;Stephens RW .Loss of ELISA specificity due to biotinylation of monoclonal antibodies.[J].Journal of Immunological Methods,2000(1/2):91-99. |
| [6] | Rowe CA.;Feldstein MJ.;Golden JP.;Scruggs SB.;MacCraith BD.;Cras JJ.;Ligler FS.;Tender LM. .Array biosensor for simultaneous identification of bacterial, viral, and protein analytes[J].Analytical chemistry,1999(17):3846-3852. |
| [7] | MacBeath G;Schreiber S L .Printing proteins as microarrays for high -throughput function determination[J].Science,2000,289:1760-1763. |
| [8] | Ziauddin J;Sabatini D M .Microarrays of cells expressing defined cDNAs[J].Nature,2001,411:107-110. |
| [9] | Zheng G;Patolsky F;Cui Y;Wang WU;Lieber CM .Multiplexed electrical detection of cancer markers with nanowire sensor arrays[J].Nature biotechnology,2005(10):1294-1301. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%