采用一步法于室温下有选择性地将小管径的单壁碳纳米管(SWCNTs)分散于生物相容性的聚合物--壳聚糖的水溶液中,并将其滴涂于玻碳电极表面,制备出微过氧化物酶-11(MP-11)修饰电极.循环伏安结果表明SWCNq、促进了MP-11在电极表面的直接电子传递,在pH=7.2的磷酸缓冲溶液中,MP-11的式电位为-0.36 V(vs.SCE),MP-11在电极表面的直接电子转移表观速率常数和覆盖度分别为78 s-1和8.76×10-10mol·cm-2.进一步的研究结果显示,固定在SWCNT表面的MP-11能保持其对氧气和过氧化氢还原的生物电催化活性,适合用作生物燃料电池的阴极和过氧化氢传感器.氧气在该修饰电极上的还原经历一个四电子过程;该过氧化氢生物传感器对过氧化氢还原的检测具有响应灵敏度高(响应时间小于4 S),检测线性范围为2.5×10-6～7.0×10-3vM,检测限为0.8 μM,相应的米氏常数和检测灵敏度分别为1.0 mM and 22.4μA/mM.
One-step aqueous dispersion and diameter- selective separation of HiPco single -walled carbon nanotubes (SWCNTs) has been accomplished through noncovalent complexation of the nanotubes with a water -soluble, biocompatible polymer chitosan (CHI) at room temperature. "Smaller -diameter" SWCNTs are preferentially dispersed and wrapped by CHI in the aqueous supema "tant that results following noncentrifuged precipitation, while "larger- diameter" SWCNTs exist in the precipitate. Such CHI wrapped small -diameter individual SWCNTs have been used for the immobilization of mieroperoxidase - 11 ( MP - 11 ) for direct elec-trochemistry and electrocatalytic purposes. We found that SWCNTs facilitate the direct electron transfer be-tween MP - 11 and the electrode surface, with a formal potential of MP - 11 at about - 0.36 V (vs. SCE) in phosphate buffer solution (pH 7.2). The heterogenous electron transfer rate constant and surface coverage of MP-11 are estimated to be 78 s-1 and 8.76 x 10-10 mol/cm2, respectively, Further study demonstrates that the modified electrode retains its electrocatalytic activity toward the reduction of both 02 and H2O2. The im-mobilized MP- 11 can catalyze O2 through a four -electron process to water, which could be used as a cath-ode in a bio fuel cell. Such SWCNT based biosensor exhibits a rapid response time, of less than 4 s, and a good linear detection range, for H2O2 concentration, from 2.5 μM to 70 μM with a detection limit of 0.8 μM. The apparent Michaelis - Menten constant (Km ) and the maximum electrode sensitivity ( Imax/Km ) are evalua-ted to be 1.0 mM and 22.4 μA/mM, respectively.
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