癌症是影响人类健康的重大疾病之一,如何快速、方便地分离癌细胞已经成为制约临床医学和科学研究的重要问题.本文将磁珠技术与MEMS技术相结合,提出了一种等边三角排列的Ni微磁柱结构,在磁场作用下使用CD326免疫磁珠捕获结肠癌细胞.本文对MEMS细胞捕获芯片进行了设计、加工和封装,采用该芯片对与磁珠结合的结肠癌细胞进行了捕获,0.1ml/h流速下捕获效率达92%,证明该芯片具有对结肠癌细胞的捕获能力.
参考文献
| [1] | 徐辉,张国亮,张凤宝.免疫磁性微球的研究进展[J].化学工业与工程,2003(01):27-32,58. |
| [2] | Xuanhong Cheng;Daniel Irimia;Meredith Dixon .A microfluidic device for practical label-free CD4+ T cell counting of HIV-infected subjects[J].Lab on a chip,2007(2):170-178. |
| [3] | Sunitha Nagrath;Lecia V. Sequist;Shyamala Maheswaran;Daphne W. Bell;Daniel Irimia;Lindsey Ulkus;Matthew R. Smith;Eunice L. Kwak;Subba Digumarthy;Alona Muzikansky .Isolation of rare circulating tumour cells in cancer patients by microchip technology[J].Nature,2007(7173):1235-1239. |
| [4] | Adams AA;Okagbare PI;Feng J;Hupert ML;Patterson D;Gottert J;McCarley RL;Nikitopoulos D;Murphy MC;Soper SA .Highly efficient circulating tumor cell isolation from whole blood and label-free enumeration using polymer-based microfluidics with an integrated conductivity sensor[J].Journal of the American Chemical Society,2008(27):8633-8641. |
| [5] | Neild A;Oberti S;Radziwill G;Dual J .Simultaneous positioning of cells into two-dimensional arrays using ultrasound[J].Biotechnology and Bioengineering,2007(5):1335-1339. |
| [6] | William Liu;Nikolai Dechev;Ian G. Foulds .A novel permalloy based magnetic single cell micro array[J].Lab on a chip,2009(16):2381-2390. |
| [7] | 潘欣欣,陈翔,陈迪,朱军,高峰.新型纳米磁色谱微流体芯片[J].功能材料与器件学报,2008(02):441-443. |
| [8] | 王聿佶,陈翔,潘欣欣,金庆辉,赵建龙.集成高梯度磁场分离结构的微流控芯片快速制作法[J].功能材料与器件学报,2008(04):751-756. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%