探讨12C6+离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒(AdCMV-GFP)转染小鼠黑色素瘤细胞(B16细胞系)的影响.采用不同剂量的12C6+重离子束辐射经AdCMV-GFP转染的B16细胞,利用流式细胞仪检测腺病毒的转染率.结果表明,12C6+重离子束辐射能提高腺病毒对B16细胞的转染率,且具有量效关系.此外,先转染后辐射法比起先辐射后转染法能更显著地提高转染率.
参考文献
| [1] | Rochlitz C F.Swiss Med Wkly,2001,131(1-2):4. |
| [2] | Huang Wenlin,Editor.Molecular Virology.Beijing:People's Medical Publishing House,2002,247-248(in Chinese).(黄文林主编.分子病毒学.北京:人民卫生出版社,2002:247-248.) |
| [3] | Mittal S K,Bett A J,Prevec L,et al.Virology,1995,210(1):226. |
| [4] | Dang Bingrong,Li Wenjian,Ma Qiufeng,et al.Nuclear Physics Review,2005,22(1):44(in Chinese).(党秉荣,李文建,马秋峰等.原子核物理评论,2005,22(1):44.) |
| [5] | Li Wenjian,Zhou Guangming,Wei Zengquan,et al.High Energy Physics and Nuclear Physics,2002,26(7):742(in Chinese).(李文建,周光明,卫增泉等.高能物理与核物理,2002,26(7):742.) |
| [6] | Bonnekoh B,Greenhalgh D A,Chen S H,et al.Adv Exp Med Biol,1998,451:335. |
| [7] | Stevens C W,Zeng M,Cerniglia G J.Hum Gene Ther,1996,7(14):1 727. |
| [8] | Webb M,Brun M,McNiven M,et al.Br J Haematol,1998,102(3):710. |
| [9] | Perez C F,Botchan M R,Tobias C A.Radiat Res,1985,104 (2 Pt 1):200. |
| [10] | Yu Zengliang.Physics,1997,26(6):333(in Chinese).(余增亮.物理,1997,26(6):333.) |
| [11] | Iwamoto R,Fushimi K,Hiraki Y,et al.Acta Med Okayama,1997,51(1):19. |
| [12] | Zeng M,Cerniglia G J,Eck S L,et al.Hum Gene Ther,1997,8(9):1 025(in Chinese). |
| [13] | Xia Shouxuan,Editor.Radiobiology.Beijing:Mititary Medical Sciences Press,1998,130-151(in Chinese).(夏寿萱主编.放射生物学.北京:军事医学科学出版社,1998,130-151.) |
| [14] | Duan Xin,Zhang Hong.Nuclear Physics Review,2006,23(3):326(in Chinese).(段昕,张红.原子核物理评论,2006,23(3):326.) |
| [15] | Min F L,Zhang H,Li W J,et al.In Vitro Cellular & Deve-lopmental Biology-Animal,2005,41:284. |
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