为克服传统免疫隔离膜吸水膨胀、机械强度差等缺点,以钛酸丁脂为原料,采用溶胶一凝胶法以多孔钛金属为基体,制备出TiO2/多孔Ti复合膜.为避免金属基体与膜材料由于热膨胀系数的不同而引起膜的破裂,采用填堵的方法成膜.通过复合膜的渗透分离实验研究其免疫隔离效果.实验结果显示,所制得的二氧化钛/多孔钛复合膜经过3次以上涂膜能有效地阻隔免疫球蛋白及免疫细胞,但小分子物质(如葡萄糖)能自由通过该膜,故该复合膜可以作为免疫隔离膜使用,并且具有良好的免疫隔离效果.
参考文献
| [1] | DesaiT A;HamsfordD;Ferrari M .Characterization of micromachined silieon membranes for immunoisolation and bioseparation applications[J].Journal of Membrane Science,1999,159(1 -2):221 -2231. |
| [2] | Opara E C;Kendall WF Jr .Immunoisolation techniques for islet cell transplantation[J].Expert Opinion on Biological Therapy,2002,2(05):503 -5511. |
| [3] | Maria-Engler SS;Mares-Guia M;CorreaM L et al.Microencapsulation and tissue engineering as an alternative treatment of diabetes[J].Brazilian Journal of Medical and Biological Research,2001,34(06):691 -6697. |
| [4] | Ma X;Vacek I;Sun A .Generation of alginate -poly -1 -lysine-alginate (APA) biomicrocapsules:the relationship between the membrane strength and the reaction con-difions[J].Artificial Cells Blood Substitutes and Immobilization Biotechnology,1994,22(01):43-69. |
| [5] | 林乐岷,宋一民,宋纯,许评,宋春芳.Successful xenotransplantation of microencapsulated newborn pig parathyroid cells in the treatment of hypoparathyroidism in rats[J].中华医学杂志(英文版),2003(08):1161-1165. |
| [6] | Desai TA;West T;Cohen M;Boiarski T;Rampersaud A .Nanoporous microsystems for islet cell replacement.[J].Advanced drug delivery reviews,2004(11):1661-1673. |
| [7] | 张守臣,王立秋,刘长厚.多孔金属Ti-SiO2复合膜的制备及在超滤分离蛋白质中的应用[J].膜科学与技术,2002(03):60-62,66. |
| [8] | 陈大鹏,郭国霖,李崇嘉.复合二氧化钛膜的研制[J].膜科学秘技术,2000(03):1-3,13. |
| [9] | Leoni L.;Desai T.A. .Nanoporous biocapsules for the encapsulation of insulinoma cells: biotransport and biocompatibility considerations[J].IEEE Transactions on Biomedical Engineering,2001(11):1335-1341. |
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