为了研究二氧化钛在免疫隔离技术中的应用,采用溶胶-凝胶法在不同烧结温度下,以多孔钛为基底制备出金红石和锐钛矿两种不同晶型的二氧化钛(TiO_2)膜,并将大鼠下丘脑神经细胞接种到TiO_2膜上,并通过体外培养观察这两种晶型的TiO_2对神经细胞生长的影响.实验结果显示,在金红石型TiO_2膜上生长的神经细胞不仅具有典型的神经细胞结构,而且能正常分泌β-内啡肽,使细胞外液中的β-内啡肽含量始终维持在42.5～45.0 pmol/L;体外培养的第7天,神经细胞经台盼蓝染色检测其存活率为86.7%,并且细胞突起交错成网;但是在锐钛矿型TiO_2膜上生长的下丘脑神经细胞则多数死亡,其存活率仅为5.3%,而少量存活的细胞不仅没有典型的神经细胞结构,而且逐渐丧失了分泌内啡肽的功能.对比结果显示金红石型TiO_2比锐钛矿型TiO_2更有利于神经细胞的生长.
The Effect of TiO_2 membranes with different phase composition on the adherent cells growth were observed by hypothalamic neurons culture in vitro for the immunoisolation application of TiO_2 membranes. The TiO_2 membranes with different phase composition supported on porous Ti planar were prepared under different sintering temperature by sol - gel technique. The results showed that the hypothalamic neurons could grow well on the TiO_2 membranes with rutile phase. These neurons not only had typical nerval morphological characters,but also kept its secretory function of β - endorphin. The contents of β - endorphin remained between 42. 5 pmol/L and 45.0 pmol/L. The livability of neurons, which were cultured on the TiO_2 with rutile phase, was 86.7%. At the same time, the hypothalamic neurons, which were cultured on the TiO_2 membranes with anatase phase, mostly died and the surviving neurons (5.3%) had neither normal nerval morphological charac-ters nor secretory function. These results indicated that the TiO_2 membranes with rutile phase were more propitious to adherent cells growth than that with rutile phase.
参考文献
| [1] | Opara E C;Kendall W F Jr .Immunoisolation techniques for islet cell transplantation[J].Expert Opinion on Biological Therapy,2002,2(05):503-511. |
| [2] | Maria-Engler S S;Mares -Guia M;Correa M L et al.Microencapsulation and tissue engineering as an alternative treatment of diabetes[J].Brazilian Journal of Medical and Biological Research,2001,34(06):691-697. |
| [3] | Leoni L;Desai T A .Nanoporous biocapsules for the encapsulation of insulinoma cells:biotransport and biccompatibility considerations.Biomedical Engineering[J].IEEE Transactions,2001,46(11):1335-1341. |
| [4] | Leoni L;Desai TA .Micromachined biocapsules for cell-based sensing and delivery.[J].Advanced drug delivery reviews,2004(2):211-229. |
| [5] | 陈大鹏,郭国霖,李崇嘉.复合二氧化钛膜的研制[J].膜科学秘技术,2000(03):1-3,13. |
| [6] | 刘锦平,史志琴.纳米二氧化钛的杀菌性能测试[J].无机盐工业,2004(04):54-55. |
| [7] | 史载锋,任学昌,孔令仁.TiO2膜光催化水处理设备的研制和应用[J].膜科学与技术,2007(04):60-65. |
| [8] | 郑志竑;林玲.神经细胞培养理论与实践[M].北京:科学出版社,2003:1-20. |
| [9] | 王廷华;冯忠堂;Ling E A.神经细胞培养理论与技术[M].北京:科学出版社,2005:1-20. |
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