对陶瓷膜过滤丁二酸发酵液的条件进行研究.考察在不同压差下,发酵液的pH以及浓缩倍数对膜通量的影响,并对膜清洗条件进行选择.结果表明在0.3 MPa压差下,发酵液调节pH至4.5,可获得最大膜通量,浓缩到7倍最佳.菌体截留率达到98.37%,蛋白质去除率为89.67%,总有机碳下降约40%.而膜污染后先后用1%次氯酸钠,1%氢氧化钠+0.5%EDTA清洗,可以恢复膜通量.实验表明,可以将陶瓷膜用于分离丁二酸发酵液,有利于简化操作步骤,提高生产效率.
The conditions of ultrafiltrate succinic acid by ceramic membrane was researched.The influences of pressure, pH and concentration ratio of fermentation on the membrane flux were studied.The optimal membrame cleaning methods were chosen.The result showed that under pressure 0.3 MPa and the fermentation broth of pH 4.5, the maximum membrane flux was attained.The cell retention rate of 98.73% retained, the removal of protein was 89.67%, and the contain of the total organic carbon declined about 40%, respectively.For cleaning the contaminated membrane, its flux could be restored by using 1% sodium hypochlorite, 1% sodium hydroxide + 0.5% EDTA washing, in turn.This work shows that ceramic membrane could be used for separating succinic acid fermentation broth, which helps to simplify the operation steps and increase productivity.
参考文献
| [1] | B.K. Nandi;R. Uppaluri;M.K. Purkait .Preparation And Characterization Of Low Cost Ceramic Membranes For Micro-filtration Applications[J].Applied clay science,2008(1/2):102-110. |
| [2] | Hsieh H P.Inorganic membranes for separation and reaction[M].The Netherlands:Elsevier,Amsterdam,1996 |
| [3] | A.M.Brites Alves;A.Morao;J.P.Cardoso .Isolation of antibiotics from industrial fermentation broths using membrane technology[J].Desalination: The International Journal on the Science and Technology of Desalting and Water Purification,2002(1/3):181-186. |
| [4] | Ohashi R;Yamamoto T;Suzuki T.Continuous production of lactic acid from molasses by perfusion culture of Lactococcus lactis using a stirred ceramic membrane reactor[J].J Biosci and Bioeng,1999(87):647-654. |
| [5] | 王庆昭,吴巍,赵学明.生物转化法制取琥珀酸及其衍生物的前景分析[J].化工进展,2004(07):794-798. |
| [6] | Hyohak Song;Sang Yup Lee .Production of succinic acid by bacterial fermentation[J].Enzyme and Microbial Technology,2006(3):352-361. |
| [7] | 吴昊,姚忠,姜岷,刘辉,陈可泉,韦萍.丁二酸发酵液的膜分离过程研究[J].食品与发酵工业,2007(05):42-46. |
| [8] | 姜岷,李建,陈可泉,王益娜,马江锋,韦萍.氧化还原电位调控对Actinobacillus succinogenes 厌氧发酵产丁二酸的影响[J].化工进展,2008(08):1250-1253,1261. |
| [9] | 余瑞元;袁明秀;陈丽蓉.生物化学实验原理和方法[M].北京:北京大学出版社,2005:240-242. |
| [10] | Aleksandro Z A;Tonikovich Q E .Method for increasing calcium succinate,calcium malate and calcium citrate solubility for child's medicinal formulations[P].RU Pat,2006114252,2007-12-27. |
| [11] | 徐志宏,魏振承,张雁,池建伟,张名位.几种蛋白质功能性质的比较研究[J].食品科学,2006(12):249-252. |
| [12] | 张建东,蔡昭铃,欧阳藩,倪晋仁.微滤过程中蛋白质聚集体的形成[J].水处理技术,2005(06):15-19. |
| [13] | Brian J Schoeman .Inorganic membranes for separation and reactions[J].Zeolites,1997,19(04):312-313. |
| [14] | 周德庆.微生物学实验手册[M].上海:上海科学技术出版社,1986:85-88. |
| [15] | 施银桃,夏东升,李海燕,曾庆福.总有机碳的测定及其在染料废水监测中的应用[J].中国环境监测,2003(04):39-42. |
| [16] | 张国瑛,顾正彪,洪雁.焦糖色素生产及应用的进展[J].食品工业科技,2007(04):232-235,238. |
| [17] | 曾坚贤,邢卫红,徐南平.陶瓷膜处理肌苷发酵液的研究[J].膜科学与技术,2004(03):23-27. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%