{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":5,"startPagecode":1},"records":[{"abstractinfo":"锌离子在胰岛素中扮演着非常重要的角色,这是因为通过金属离子与蛋白质之间的相互作用,使得胰岛素的结构发生变化并影响胰岛素的生物功能.因此,去锌胰岛素在金属和蛋白识别以及结构研究中是必需的.该文采用乙二胺四乙酸(EDTA)二钠盐与锌离子进行配合作用形成稳定的[Zn-EDTA]2-配合物来去除胰岛素中的锌离子,然后用Sephadex G-25柱对体系进行凝胶过滤,以除去胰岛素中的配合物和原有的苯酚,并用电喷雾离子化质谱对分离的物质进行鉴定.结果显示除去锌离子的胰岛素与EDTA配合物的分离效果良好.采用原子吸收光谱来检验胰岛素馏分中锌离子的去除效果,结果显示94.3%的锌离子被去除,表明EDTA配合锌离子的效果显著.所有结果均表明该方法可用来制备去锌牛胰岛素.","authors":[{"authorName":"<span style=\"color:red\">陈</span><span style=\"color:red\">建</span><span style=\"color:red\">锋</span>","id":"b1e4bed0-23fd-40e8-839e-fc3c4d5928b3","originalAuthorName":"陈建锋"},{"authorName":"张薇","id":"3dbe8db4-85c1-47b5-a6eb-4e5c255d8545","originalAuthorName":"张薇"},{"authorName":"樊柳荫","id":"53c9926a-0625-4520-a54b-79ecf47a34c5","originalAuthorName":"樊柳荫"},{"authorName":"任吉存","id":"824c9bfa-0ce3-47b9-8c93-d88473598b5b","originalAuthorName":"任吉存"},{"authorName":"曹成喜","id":"b310fa58-2ed0-4da0-9cbb-9669a996e376","originalAuthorName":"曹成喜"}],"doi":"10.3321/j.issn:1000-8713.2007.06.039","fpage":"955","id":"e65c4e38-1bab-41c6-a983-503ad4b5cdd6","issue":"6","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"f3408092-d9bf-49e3-9ae4-3a7ee6e3179d","keyword":"凝胶色谱(gel chromatography)","originalKeyword":"凝胶色谱(gel chromatography)"},{"id":"0c4b1be9-5447-496c-b684-1d7fc24ff928","keyword":"电喷雾离子化质谱(ESI-MS)","originalKeyword":"电喷雾离子化质谱(ESI-MS)"},{"id":"90ffa13f-f0a0-47d1-a012-5ccd30bef368","keyword":"原子吸收光谱(AAS)","originalKeyword":"原子吸收光谱(AAS)"},{"id":"8db0841a-5b17-481c-b7d2-0a9b011c2c2c","keyword":"乙二胺四乙酸(EDTA)","originalKeyword":"乙二胺四乙酸(EDTA)"},{"id":"9ffdfe43-5c6d-44f0-9b98-48af5a609515","keyword":"去锌胰岛素(zinc-free insulin)","originalKeyword":"去锌胰岛素(zinc-free insulin)"},{"id":"6fee0ded-59bd-40ed-a7db-780e963aa03d","keyword":"制备(preparation)","originalKeyword":"制备(preparation)"},{"id":"86f6be38-ff70-4697-b291-e6f93fb6f692","keyword":"鉴定(identification)","originalKeyword":"鉴定(identification)"}],"language":"zh","publisherId":"sp200706039","title":"去锌牛胰岛素的制备和鉴定","volume":"25","year":"2007"},{"abstractinfo":"在力化学法钛白聚苯乙烯固相接枝改性工艺的基础上,应用多种检测手段研究了力化学法钛白聚苯乙烯固相接枝改性的机理,并建立了钛白表面聚苯乙烯接枝模型,认为力化学法钛白聚苯乙烯固相接枝改性的机理主要是自由基聚合反应.","authors":[{"authorName":"毋伟","id":"764b0e29-988f-4395-bc1f-0245cd166fed","originalAuthorName":"毋伟"},{"authorName":"<span style=\"color:red\">陈</span><span style=\"color:red\">建</span><span style=\"color:red\">锋</span>","id":"6165e384-6edf-49f5-96ba-74f0bba671f6","originalAuthorName":"陈建锋"},{"authorName":"卢寿慈","id":"8baba3d1-1832-4098-92d1-dd9dfee17a38","originalAuthorName":"卢寿慈"}],"doi":"","fpage":"30","id":"9d6ae801-703d-4cf7-b412-6ea2d0096426","issue":"3","journal":{"abbrevTitle":"GFZCLKXYGC","coverImgSrc":"journal/img/cover/GFZCLKXYGC.jpg","id":"31","issnPpub":"1000-7555","publisherId":"GFZCLKXYGC","title":"高分子材料科学与工程"},"keywords":[{"id":"ac812643-4442-46b2-a326-e60e87e9eae3","keyword":"力化学","originalKeyword":"力化学"},{"id":"902f771d-f373-439c-80ec-ea726b8d3d9f","keyword":"钛白","originalKeyword":"钛白"},{"id":"4627f9e5-16c8-4d9a-a097-ab743e1ffbc2","keyword":"聚苯乙烯","originalKeyword":"聚苯乙烯"},{"id":"a8d44148-ccc0-41c7-b564-c72d4bc7c083","keyword":"接枝模型","originalKeyword":"接枝模型"},{"id":"d99d2472-4371-4d50-9cb5-0cd2a817af28","keyword":"机理","originalKeyword":"机理"}],"language":"zh","publisherId":"gfzclkxygc200203007","title":"力化学法钛白聚苯乙烯固相接枝改性机理研究","volume":"18","year":"2002"},{"abstractinfo":"","authors":[],"doi":"","fpage":"354","id":"7383e1b0-3ed1-4def-8416-6d0851c6fb9b","issue":"6","journal":{"abbrevTitle":"DDYTS","coverImgSrc":"journal/img/cover/DDYTS.jpg","id":"21","issnPpub":"1004-227X","publisherId":"DDYTS","title":"电镀与涂饰   "},"keywords":[{"id":"d56430c5-665e-474a-bd95-22b7e0d8d52f","keyword":"","originalKeyword":""}],"language":"zh","publisherId":"ddyts201506018","title":"《地坪涂料与涂装技术》（<span style=\"color:red\">陈</span>文广、梁剑<span style=\"color:red\">锋</span>、周子鹄等编著）等","volume":"","year":"2015"},{"abstractinfo":"采用双<span style=\"color:red\">锋</span>角钻头和普通麻花钻对T700碳纤维复合材料(CFRP)进行钻削试验,从钻削轴向力、制孔出口质量和表面粗糙度等方面分析双<span style=\"color:red\">锋</span>角钻头在不同加工参数下制孔特点,并与普通麻花钻进行对比.试验结果表明:与普通麻花钻对比,双<span style=\"color:red\">锋</span>角钻头钻削CFRP时钻削轴向力减小约20％,制孔出口质量更好,孔壁的表面粗糙度值减小,体现优异的切削性能更适合CFRP的制孔加工.","authors":[{"authorName":"刘洋","id":"84b05ae5-a230-41ff-b807-1153de61c705","originalAuthorName":"刘洋"},{"authorName":"李鹏南","id":"ce579e61-f76f-484c-b690-a2f0dc957fae","originalAuthorName":"李鹏南"},{"authorName":"陈明","id":"55f42f01-21ff-4344-a87c-e23d5f0523bf","originalAuthorName":"陈明"},{"authorName":"邱新义","id":"46e7be67-8ed8-4643-866d-0b60fe3a811c","originalAuthorName":"邱新义"},{"authorName":"胡立湘","id":"daaff4cd-74ae-4036-9af8-a60665d7228a","originalAuthorName":"胡立湘"}],"doi":"10.3969/j.issn.1007-2330.2015.06.012","fpage":"53","id":"202f4754-37c9-42f9-afdf-6d82c39bd52e","issue":"6","journal":{"abbrevTitle":"YHCLGY","coverImgSrc":"journal/img/cover/YHCLGY.jpg","id":"77","issnPpub":"1007-2330","publisherId":"YHCLGY","title":"宇航材料工艺   "},"keywords":[{"id":"1af4de59-2086-474a-bec7-84f7ee6ae50b","keyword":"双<span style=\"color:red\">锋</span>角钻头","originalKeyword":"双锋角钻头"},{"id":"947a52d4-b948-47ab-8901-421d8ee8afe3","keyword":"CFRP","originalKeyword":"CFRP"},{"id":"24c898bd-96b6-4e32-8553-b90975356206","keyword":"钻削轴向力","originalKeyword":"钻削轴向力"},{"id":"ff98df70-d644-4a98-b0ed-ed4b53688240","keyword":"出口质量","originalKeyword":"出口质量"},{"id":"398d4903-6c1c-4810-b1cb-6542f6d54a30","keyword":"孔壁表面粗糙度","originalKeyword":"孔壁表面粗糙度"}],"language":"zh","publisherId":"yhclgy201506012","title":"双<span style=\"color:red\">锋</span>角钻头钻削碳纤维增强树脂基复合材料研究","volume":"45","year":"2015"},{"abstractinfo":"笔者根据几年来的阴极电泳涂装线<span style=\"color:red\">建</span>线经验,介绍了新建阴极电泳涂装线的清洗、<span style=\"color:red\">建</span>槽、试生产运行调试及日常管理的关键问题及注意事项.","authors":[{"authorName":"金荣刚","id":"f6f64836-f4c3-49b6-8785-d2532d9fc868","originalAuthorName":"金荣刚"}],"doi":"10.3969/j.issn.0253-4312.2003.01.009","fpage":"23","id":"ee884b3d-3ddb-44a8-8f4c-25da97c5e7e2","issue":"1","journal":{"abbrevTitle":"TLGY","coverImgSrc":"journal/img/cover/TLGY.jpg","id":"61","issnPpub":"0253-4312","publisherId":"TLGY","title":"涂料工业   "},"keywords":[{"id":"b64ef97a-ed4c-478e-bb1d-f86e946d4f72","keyword":"阴极电泳涂装线","originalKeyword":"阴极电泳涂装线"},{"id":"64e2ebc2-8483-4f22-8b18-c51548fee0d9","keyword":"清洗","originalKeyword":"清洗"},{"id":"50438fd5-ec56-42ee-ba02-5fecd4b115aa","keyword":"<span style=\"color:red\">建</span>槽","originalKeyword":"建槽"},{"id":"944199e4-aa81-4af5-a3f0-5651ad6905e8","keyword":"运行调度","originalKeyword":"运行调度"},{"id":"0d6f45ce-50c4-4751-a9e0-b66611f4ff8c","keyword":"日常管理","originalKeyword":"日常管理"}],"language":"zh","publisherId":"tlgy200301009","title":"阴极电泳涂装线<span style=\"color:red\">建</span>线方案","volume":"33","year":"2003"},{"abstractinfo":"简要介绍了中高能核数据评价和<span style=\"color:red\">建</span>库的国际概况, 较细致地讨论了中高能核反应实验测量、理论计算、积分检验、评价和<span style=\"color:red\">建</span>库等, 并对我国今后中高能核数据工作提出了建议. ","authors":[{"authorName":"庄友祥","id":"be65009e-0cb5-4fb5-bbe1-29a1c7fc9a28","originalAuthorName":"庄友祥"},{"authorName":"王记民","id":"54eacf35-7d06-49af-9779-d9755a13efb2","originalAuthorName":"王记民"}],"doi":"10.3969/j.issn.1007-4627.2002.04.013","fpage":"425","id":"176fa7e7-1207-4556-a67a-2f1d659e829c","issue":"4","journal":{"abbrevTitle":"YZHWLPL","coverImgSrc":"journal/img/cover/YZHWLPL.jpg","id":"78","issnPpub":"1007-4627","publisherId":"YZHWLPL","title":"原子核物理评论   "},"keywords":[{"id":"92a902c9-e7a0-48b5-bca3-0c850d82ed2b","keyword":"核反应","originalKeyword":"核反应"},{"id":"1177774f-fa38-4c86-b967-328aaa15e10b","keyword":"核数据","originalKeyword":"核数据"},{"id":"7e047e06-a575-4be0-ad11-ffcf840cc7c9","keyword":"中高能","originalKeyword":"中高能"},{"id":"357cfee6-1c89-4b0d-b2cd-b162248e3cb3","keyword":"评价","originalKeyword":"评价"},{"id":"aab1f2e4-69e1-420d-b2a7-d8592492de3b","keyword":"<span style=\"color:red\">建</span>数据库","originalKeyword":"建数据库"}],"language":"zh","publisherId":"yzhwlpl200204013","title":"中高能核数据评价和<span style=\"color:red\">建</span>库国际概况","volume":"19","year":"2002"},{"abstractinfo":"以能量为1.5 MeV/u，剂量分别为500，750，1000，1250，1500 Gy的电子束对1，2，4，6，8年等5种年份浓香型白酒进行辐照处理；扫描各酒样200～400 nm波段的紫外光谱，根据紫外光谱图的差异，计算了280～300 nm波段的光谱曲线相似度，分析光谱曲线变化规律。结果表明，对于前4种白酒，对照样与辐照样光谱曲线相似度值越小，催<span style=\"color:red\">陈</span>效果越好；白酒存放时间越久，酒体风格转向老熟所需剂量越小，越容易达到最佳催<span style=\"color:red\">陈</span>效果；对于8年白酒，辐照剂量超过750 Gy后，白酒体系动态平衡被打破，各单体物质增加，出现返生现象。因此，电子束辐照技术对低年份浓香型白酒催<span style=\"color:red\">陈</span>效果显著，是一种先进、高效的催<span style=\"color:red\">陈</span>方法。","authors":[{"authorName":"张苗苗","id":"16f2bee9-e276-4f55-95b1-9e627a4ac276","originalAuthorName":"张苗苗"},{"authorName":"陆栋","id":"6bd2c6ff-fa0f-4deb-9553-bfa0e40d3ab7","originalAuthorName":"陆栋"},{"authorName":"曹国珍","id":"ad44f083-8f23-4d8d-a41c-b6321e181155","originalAuthorName":"曹国珍"},{"authorName":"刘敬","id":"980de600-54b3-4c10-b2b1-7d36c88bd704","originalAuthorName":"刘敬"},{"authorName":"金文杰","id":"9d97782b-4400-438f-aa1a-efaa2ae74455","originalAuthorName":"金文杰"},{"authorName":"王菊芳","id":"7a171c96-4419-441c-8118-cabb35cc1b2c","originalAuthorName":"王菊芳"},{"authorName":"李文<span style=\"color:red\">建</span>","id":"db0225a5-b939-440c-a47d-2daa37f339b7","originalAuthorName":"李文建"}],"doi":"10.11804/NuclPhysRev.31.02.218","fpage":"218","id":"be399f10-d5ab-444a-9853-693337e4845a","issue":"2","journal":{"abbrevTitle":"YZHWLPL","coverImgSrc":"journal/img/cover/YZHWLPL.jpg","id":"78","issnPpub":"1007-4627","publisherId":"YZHWLPL","title":"原子核物理评论   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style=\"color:red\">陈</span>向新","id":"f5088ffa-c534-4a55-a2c7-af2943bdef19","originalAuthorName":"陈向新"},{"authorName":"薛致远等","id":"d6eaf0b1-e472-4b82-b187-8b7181d319f0","originalAuthorName":"薛致远等"}],"categoryName":"|","doi":"","fpage":"227","id":"c75a8f29-bb62-4950-aaf4-f57c687be0a2","issue":"4","journal":{"abbrevTitle":"FSXB","coverImgSrc":"journal/img/cover/腐蚀学报封面.jpg","id":"24","issnPpub":"2667-2669","publisherId":"FSXB","title":"腐蚀学报（英文）"},"keywords":[{"id":"c16731a9-d142-499f-9735-a75aa9539cc6","keyword":"已<span style=\"color:red\">建</span>油库管道","originalKeyword":"已建油库管道"},{"id":"b53f7e22-9e58-495f-8892-69cc9f779150","keyword":"oil store","originalKeyword":"oil store"},{"id":"bd103846-d013-43c3-869a-040cf34c6070","keyword":"cathodic protection","originalKeyword":"cathodic protection"}],"language":"zh","publisherId":"1002-6495_1999_4_5","title":"已<span style=\"color:red\">建</span>油库埋地管道阴极保护","volume":"11","year":"1999"},{"abstractinfo":"对已<span style=\"color:red\">建</span>油库埋地管道进行阴极保护,解决了埋地30多年的老管道的腐蚀控制问题.","authors":[{"authorName":"胡士信","id":"a2dbac43-47e3-4a41-b502-ad915c27da15","originalAuthorName":"胡士信"},{"authorName":"<span style=\"color:red\">陈</span>向新","id":"3cea5cd7-8913-4d17-8f19-fd4ea5e6d93e","originalAuthorName":"陈向新"},{"authorName":"薛致远","id":"349260e2-b7e4-4ea6-b8b0-c038d1febc58","originalAuthorName":"薛致远"},{"authorName":"张志远","id":"a01aa0d4-be9b-4175-89bd-fd8874932b2c","originalAuthorName":"张志远"},{"authorName":"李永亮","id":"cc63858a-357b-437f-8480-ac2b095cfc40","originalAuthorName":"李永亮"},{"authorName":"许卫新","id":"deb55f3e-d111-47c2-ab9c-7e26277376a0","originalAuthorName":"许卫新"},{"authorName":"谢永发","id":"a14d8624-2dc2-4e68-9306-24dd58e9fc2a","originalAuthorName":"谢永发"}],"doi":"10.3969/j.issn.1002-6495.1999.04.007","fpage":"227","id":"3fd29048-3d99-4eb5-b602-7fb91514dfda","issue":"4","journal":{"abbrevTitle":"FSXB","coverImgSrc":"journal/img/cover/腐蚀学报封面.jpg","id":"24","issnPpub":"2667-2669","publisherId":"FSXB","title":"腐蚀学报（英文）"},"keywords":[{"id":"f72e959c-0998-4df1-838b-3332118651a2","keyword":"已<span 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style=\"color:red\">建</span>龙钢铁石灰-石膏法湿式脱硫工艺流程,并从工程建设角度,对选择该工艺后可能出现的腐蚀和磨损问题进行了应对策略分析.","authors":[{"authorName":"芮义斌","id":"7e6f7537-1190-450f-b712-0daea3f79f78","originalAuthorName":"芮义斌"},{"authorName":"赵宏","id":"ed80e639-fd34-4da6-b597-d3cbc01d986b","originalAuthorName":"赵宏"}],"doi":"10.3969/j.issn.1000-6826.2010.01.017","fpage":"39","id":"bac2fe71-756b-485c-b983-118325c8c6fe","issue":"1","journal":{"abbrevTitle":"JSSJ","coverImgSrc":"journal/img/cover/3abe017a-2574-4821-8152-4ae974ef0471.jpg","id":"47","issnPpub":"1000-6826","publisherId":"JSSJ","title":"金属世界"},"keywords":[{"id":"6d9408a7-41ff-4af6-8933-87226c1411b8","keyword":"烧结机","originalKeyword":"烧结机"},{"id":"749c272e-fa1f-4079-af32-e7887bc283fd","keyword":"烟气","originalKeyword":"烟气"},{"id":"ee3945cb-20c9-4847-8872-a779deca0d2a","keyword":"脱硫","originalKeyword":"脱硫"},{"id":"093e7a7c-4e78-4041-9552-d7162b694458","keyword":"磨损","originalKeyword":"磨损"},{"id":"01e87954-8644-42dd-a1d6-13a34b686ed0","keyword":"腐蚀","originalKeyword":"腐蚀"}],"language":"zh","publisherId":"jssj201001017","title":"<span style=\"color:red\">建</span>龙钢铁烧结烟气脱硫工艺抉择实践","volume":"","year":"2010"}],"totalpage":14,"totalrecord":131}