利用圆二色光谱测定大腹园蛛的丝蛋白,结果显示蜘蛛丝溶液中的构象最初是无规卷曲和β折叠,同时用拉曼光谱对蜘蛛吐丝口附近的牵引丝及垂直下落的蜘蛛牵引丝进行比较,结果表明在蜘蛛纺丝过程中无规卷曲转变为α螺旋,并且垂直下落的蜘蛛牵引丝由于受到空气中的拉伸其β折叠取向比吐丝口附近的牵引丝好.这些分子结构的多样性决定了蜘蛛牵引丝优异的机械性能.
The Circular dichroism(CD) spectrum of spider silk fibroin of Araneus Ventricosus major ampullate glands was measured,which indicated that the molecular conformations of spider silk in solution were primarily random coils and β-sheets.The Raman spectra were recorded for dragline silks next to the spigot and spun by a dropping spider.The results showed thatrandom coils were transformed into α-helix in the spider spinning process,andthat β-sheet orientation in dragline silk of a dropping spider was better than that of at the spigot because of dragging in air.The multiplicity of molecularstructures contributed excellent mechanical properties of dragline silk.
参考文献
| [1] | Griffiths JR;Salanitri VR .Strength of spider silk[J].Journal of Materials Science,1980,15:491-496. |
| [2] | Kitagawa M.;Kitayama T. .MECHANICAL PROPERTIES OF DRAGLINE AND CAPTURE THREAD FOR THE SPIDER NEPHILA CLAVATA[J].Journal of Materials Science,1997(8):2005-2012. |
| [3] | Moore AMF;Tran K .Material properties of cobweb silk produced by the black widow spider Latrodectus mactans[J].American Zoologist,1996,36:54A. |
| [4] | Work RW;Young CT J .The amino acid composition of major and minorampullate silks of certain orb-web-building spiders[J].Arachnol,1987,15:65-80. |
| [5] | Xu M;Lewis RV .Structure of aprotein superfiber spider dragline silk[J].Proceedings of the National Academy of Sciences of the United States of America,1990,87:71207124. |
| [6] | Lewis RV.;Kothakota S.;Fournier MJ.;Hinman M. .EXPRESSION AND PURIFICATION OF A SPIDER SILK PROTEIN - A NEW STRATEGY FOR PRODUCING REPETITIVE PROTEINS[J].Protein Expression and Purification,1996(4):400-406. |
| [7] | Hayashi CY.;Lewis RV. .Evidence from flagelliform silk cDNA for the structural basis of elasticity and modular nature of spider silks[J].Journal of Molecular Biology,1998(5):773-784. |
| [8] | Kummerlen J.;Vollrath F.;Meier BH.;Vanbeek JD. .LOCAL STRUCTURE IN SPIDER DRAGLINE SILK INVESTIGATED BY TWO-DIMENSIONAL SPIN-DIFFUSION NUCLEAR MAGNETIC RESONANCE[J].Macromolecules,1996(8):2920-2928. |
| [9] | Simmons AH;Ed Ray;Jelinskit LW .Solid-State 13C NMR of Nephlia Clavipes Dragline Silk Establishes Structure and Identity of Crystalline Regions[J].Macromolecules,1994,27:5235-5237. |
| [10] | Kitagawa M.;Katsumi S. .Structure and Deformation of Dragline and Capture Threads of Orb-Web in Nephila Clavate[J].材料,1997(4):470-474. |
| [11] | Bram A.;Craig C.;Snigireva I.;Riekel C.;Branden CI. .X-RAY DIFFRACTION FROM SINGLE FIBRES OF SPIDER SILK[J].Journal of Applied Crystallography,1997(3):390-392. |
| [12] | Hijirida DH;Gian Do K;Michal C;Shan W,Zax D,and Jelinski LW .13C NMR of Nephlia Clavipes Major Ampullate Silk Gland[J].BIOPHYSICAL JOURNAL,1996,71:3442-3447. |
| [13] | Termonia Y .Molecular modeling of spider silk elasticity[J].Macromolecules,1994,27:7378-7381. |
| [14] | Lazaris A;Areidiacono S;Huang Y et al.Spider silk fibers spun from frcombinant silk produced in Mammalian Cells[J].Science,2002,295:472-476. |
| [15] | Livak O;Blye A et al.A microfabricated wet-spinning apparatus to spin fibers of silk protein.Structure-property correlation[J].Macromolecules,1998,31:2947-2951. |
| [16] | Seidel A;Liivak O;Jelinskit L W .Artificial Spinning of Spider Silk[J].Macromolecules,2000,33:775-780. |
| [17] | Vollrath F;Knight DP .Liquid crystalline spinning of spider silk [Review][J].Nature,2001(6828):541-548. |
| [18] | Garrido MA.;Elices M.;Viney C.;Perez-Rigueiro J. .Active control of spider silk strength: comparison of drag line spun on vertical and horizontal surfaces[J].Polymer: The International Journal for the Science and Technology of Polymers,2002(4):1537-1540. |
| [19] | Knight DP;Vollrath F .Liquid crystal and flow elongation in a spider′s silk production line[J].Proceedings of the Royal Society B:Biological Sciences,1999,266:519-523. |
| [20] | Willcox PJ.;Muller W.;Kaplan DL.;Gido SP. .EVIDENCE OF A CHOLESTERIC LIQUID CRYSTALLINE PHASE IN NATURAL SILK SPINNING PROCESSES[J].Macromolecules,1996(15):5106-5110. |
| [21] | Kerkam K;Viney C;Kaplan D .Liquid crystallinity of natural silk secretions[J].Nature,1991,349:596-598. |
| [22] | Vollrath F;Knight DP .Structure and function of the silk production pathway in the spider Nephlia edulis[J].International Journal of Biological Macromolecules,1999,24:243-249. |
| [23] | Knight D;Vollrath F .Hexagonal columnar liquid crystal in the cells secreting spider silk[J].Tissue and Cell,1999(6):617-620. |
| [24] | Wang SY.Fiber Reinforced Composites[M].Shanghai China:China Textile University Press,1998:11-30. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%