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本文用溶胶-凝胶法在塑料表面制作二氧化硅涂层,并利用制作的二氧化硅层来提取DNA.利用这种方法制作了塑料基的DNA提取芯片,并将该芯片应用于从人血中提取DNA.结果表明:经过溶胶涂渍、在内表面制作二氧化硅层的芯片能够实现从血液中提取DNA,而没有经过溶胶涂渍的芯片无法提取DNA.涂渍的芯片所提取出的DNA能够满足聚合酶链反应的需要.

参考文献

[1] Burns,MA;Johnson,BN;Brahmasandra,SN;Handique,K;Webster,JR;Krishnan,M;Sammarco,TS;Man,PM;Jones,D;Heldsinger,D;Mastrangelo,CH;Burke,DT .An integrated nanoliter DNA analysis device (see comments)[J].Science,1998(5388):484-487.
[2] Liying Yao;Baoan Liu;Too Chen;Shibing Liu;Tiechuan Zuo .Micro Flow-through PCR in a PMMA Chip Fabricated by KrF Excimer Laser[J].Biomedical microdevices,2005(3):253-257.
[3] Fixe F;Dufva M;Telleman P et al.Functionalization of poly (methyl methacrylate) (PMMA) as a substrate for DNA microarrays[J].Nucleic Acids Research,2004,32(01):e9-e9.
[4] Lin Y W;Chang H T .Modification of poly(methyl methacrylate) microchannels for highly efficient and reproducible electro-phoretic separations of double-stranded DNA[J].Journal of Chromatography A,2005,1073:191-199.
[5] Vilkner T;Janasek D;Manz A .Micro total analysis systems.Recent developments[J].Analytical Chemistry,2004,76:3373-3386.
[6] Hong JW;Studer V;Hang G;Anderson WF;Quake SR .A nanoliter-scale nucleic acid processor with parallel architecture[J].Nature biotechnology,2004(4):435-439.
[7] Melzak KA.;Turner RFB.;Haynes CA.;Sherwood CS. .DRIVING FORCES FOR DNA ADSORPTION TO SILICA IN PERCHLORATE SOLUTIONS[J].Journal of Colloid and Interface Science,1996(2):635-644.
[8] Vogelstein B;Gillespie D.Preparative and analytical purification of DNA from agarose[A].,1979:76,615-619.
[9] Breadmore MC.;Wolfe KA.;Arcibal IG.;Leung WK.;Dickson D.;Giordano BC. Power ME.;Ferrance JP.;Feldman SH.;Norris PM.;Landers JP. .Microchip-based purification of DNA from biological samples[J].Analytical Chemistry,2003(8):1880-1886.
[10] Wolfe KA.;Breadmore MC.;Ferrance JP.;Power ME.;Conroy JF.;Norris PM. Landers JP. .Toward a microchip-based solid-phase extraction method for isolation of nucleic acids[J].Electrophoresis: The Official Journal of the International Electrophoresis Society,2002(5):727-733.
[11] Christel L A;Petersen K;McMillan W et al.Automated nucleic acid probe assays using silicon microstructures for nucleic acid concentration[J].Journal of Biomechanical Engineering-transactions of The Asme,1999,121:22-27.
[12] Cady NC.;Stelick S.;Batt CA. .Nucleic acid purification using microfabricated silicon structures[J].Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices,2003(1):59-66.
[13] Nakagawa T;Tanaka T;Niwa D;Osaka T;Takeyama H;Matsunaga T .Fabrication of amino silane-coated microchip for DNA extraction from whole blood[J].Journal of Biotechnology,2005(2):105-111.
[14] Becker H.;Gartner C. .Polymer microfabrication methods for microfluidic analytical applications [Review][J].Electrophoresis: The Official Journal of the International Electrophoresis Society,2000(1):12-26.
[15] Becker H.;Heim U. .Hot embossing as a method for the fabrication of polymer high aspect ratio structures[J].Sensors and Actuators, A. Physical,2000(1/3):130-135.
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