人口老龄化，疾病以及交通事故等造成大量的人体骨组织损伤和丢失。如何实现骨组织缺损的快速修复与再生成为临床医学研究的重要课题和目标，而生物医用材料在其中发挥着极其重要的作用。目前临床上常用的骨组织修复材料如自体骨、异体骨、合成材料（金属，陶瓷，高分子）等都存在各种各样的问题，无法实现大规模的应用和骨组织的快速有效再生。而骨组织工程学科研究多孔支架结合细胞和生长因子来实现骨组织再生，以解决骨科临床面临的问题为目的。最近十多年来，三维纳米纤维支架由于可以仿天然细胞外基质的结构和形态而显示出较强的促进细胞增殖、成骨分化以及骨组织修复再生的能力。主要综述具有仿生的纳米纤维及其复合支架材料的制备技术以及他们在增强细胞功能、干细胞成骨分化、及其骨组织再生中的应用。
Population aging, bone diseases and accidents result in a large number of patients with serious bone loss and defects.The efficient bone tissue repair and regeneration have been important topics in clinical medicine .Here, biomedic-al materials play an important role in bone regeneration .However, current clinical bone-repair biomaterials such as auto-grafts, allografts and synthetic materials (metals, ceramics and polymers) suffer from various shortcomings, having limit-ed applications in bone repair.In bone tissue engineering research, biodegradable scaffolds along with cells and growth factors have shown high potential in facilitating bone regeneration as a potential new therapy for bone loss in the clinic .In the past decade, due to their structure and morphology that mimic the native extracellular matrix , nanofibrous scaffolds have been shown to be capable of facilitating cell proliferation, osteogenic differentiation of stem cells, and bone regenera-tion in vivo compared to control scaffolds.In this paper, we will review the fabrication technologies of biomimetic nanofi-brous scaffolds and their applications in enhancing cellular function , osteogenic differentiation, and bone tissue regenera-tion.
参考文献
| [1] | Gruskin,E.;Doll,B.A.;Futrell,F.W.;Schmitz,J.P.;Hollinger,J.O. .Demineralized bone matrix in bone repair: History and use[J].Advanced drug delivery reviews,2012(12):1063-1077. |
| [2] | Ti based biomaterials, the ultimate choice for orthopaedic implants - A review[J].Progress in materials science,2009(3):397. |
| [3] | Marc Bohner .Resorbable biomaterials as bone graft substitutes[J].Materials Today,2010(1/2):24-30. |
| [4] | Zandonella C .Tissue Engineering:the Beat Goes on[J].Nature,2003,421(6 926):884-886. |
| [5] | Hubbell J A .Biomaterials in Tissue Engineering[J].Nature Bi-otechnology,1995,13(06):565-576. |
| [6] | Boden,SD .Bioactive factors for bone tissue engineering.[J].Clinical Orthopaedics and Related Research,1999(367 Suppl):84-94. |
| [7] | Bianco P;Robey P G .Stem Cells in Tissue Engineering[J].Nature,2001,414(6 859):118-121. |
| [8] | Crane G M;Ishaug S L;Mikos A G .Bone Tissue Engineering[J].Nature Medicine,1995,1(12):1322-1324. |
| [9] | Lutolf MP;Hubbell JA .Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering[J].Nature biotechnology,2005(1):47-55. |
| [10] | Hollister SJ .Porous scaffold design for tissue engineering[J].Nature materials,2005(7):518-524. |
| [11] | Lutolf MR;Weber FE;Schmoekel HG;Schense JC;Kohler T;Muller R .Repair of bone defects using synthetic mimetics of collagenous extracellular matrices[J].Nature biotechnology,2003(5):513-518. |
| [12] | Eileen Gentleman;Robin J. Swain;Nicholas D. Evans .Comparative materials differences revealed in engineered bone as a function of cell-specific differentiation[J].Nature materials,2009(9):763-770. |
| [13] | Holzwarth JM;Ma PX .Biomimetic nanofibrous scaffolds for bone tissue engineering.[J].Biomaterials,2011(36):9622-9629. |
| [14] | Jang JH;Castano O;Kim HW .Electrospun materials as potential platforms for bone tissue engineering.[J].Advanced drug delivery reviews,2009(12):1065-1083. |
| [15] | Ma PX .Biomimetic materials for tissue engineering.[J].Advanced drug delivery reviews,2008(2):184-198. |
| [16] | Jeremy M. Holzwarth;Peter X. Ma .3D nanofibrous scaffolds for tissue engineering[J].Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology,2011(28):10243-10251. |
| [17] | Zhong,S.;Zhang,Y.;Lim,C.T. .Fabrication of large pores in electrospun nanofibrous scaffolds for cellular infiltration: A review[J].Tissue engineering, Part B. Reviews,2012(2):77-87. |
| [18] | Blakeney BA;Tambralli A;Anderson JM;Andukuri A;Lim DJ;Dean DR;Jun HW .Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold.[J].Biomaterials,2011(6):1583-1590. |
| [19] | Wan-Ju Li;Cato T. Laurencin;Edward J. Caterson .Electrospun nanofibrous structure: A novel scaffold for tissue engineering[J].Journal of biomedical materials research, Part B. Applied biomaterials,2002(4):613-621. |
| [20] | Yang F;Murugan R;Wang S;Ramakrishna S .Electrospinning of nano/micro scale poly(l-lactic acid) aligned fibers and their potential in neural tissue engineering.[J].Biomaterials,2005(15):2603-2610. |
| [21] | Ko Eun Park;Hyun Ki Kang;Seung Jin Lee;Byung-Moo Min;Won Ho Park .Biomimetic Nanofibrous Scaffolds:Preparation and Characterization of PGA/Chitin Blend Nanofibers[J].Biomacromolecules,2006(2):635-643. |
| [22] | Li WJ;Tuli R;Huang X;Laquerriere P;Tuan RS .Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold.[J].Biomaterials,2005(25):5158-5166. |
| [23] | Shih YR;Chen CN;Tsai SW;Wang YJ;Lee OK .Growth of mesenchymal stem cells on electrospun type I collagen nanofibers.[J].Stem Cells,2006(11):2391-2397. |
| [24] | Li MY;Mondrinos MJ;Gandhi MR;Ko FK;Weiss AS;Lelkes PI .Electrospun protein fibers as matrices for tissue engineering[J].Biomaterials,2005(30):5999-6008. |
| [25] | Yang DZ;Jin Y;Zhou YS;Ma GP;Chen XM;Lu FM;Nie J .In situ mineralization of hydroxyapatite on electrospun chitosan-based nanofibrous scaffolds[J].Macromolecular bioscience,2008(3):239-246. |
| [26] | Bhattarai N;Li ZS;Edmondson D;Zhang MQ .Alginate-based nanofibrous scaffolds: Structural, mechanical, and biological properties[J].Advanced Materials,2006(11):1463-1467. |
| [27] | Li C;Vepari C;Jin HJ;Kim HJ;Kaplan DL .Electrospun silk-BMP-2 scaffolds for bone tissue engineering.[J].Biomaterials,2006(16):3115-3124. |
| [28] | Li M;Mondrinos MJ;Chen X;Gandhi MR;Ko FK;Lelkes PI .Co-electrospun poly(lactide-co-glycolide), gelatin, and elastin blends for tissue engineering scaffolds.[J].Journal of biomedical materials research, Part A,2006(4):963-973. |
| [29] | Jose, MV;Thomas, V;Dean, DR;Nyairo, E .Fabrication and characterization of aligned nanofibrous PLGA/Collagen blends as bone tissue scaffolds[J].Polymer: The International Journal for the Science and Technology of Polymers,2009(15):3778-3785. |
| [30] | Ghasemi-Mobarakeh L;Prabhakaran MP;Morshed M;Nasr-Esfahani MH;Ramakrishna S .Electrospun poly(epsilon-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering.[J].Biomaterials,2008(34):4532-4539. |
| [31] | Malheiro VN;Caridade SG;Alves N .New poly(epsilon-caprolactone)/chitosan blend fibers for tissue engineering applications.[J].Acta biomaterialia,2010(2):418-428. |
| [32] | Zhang Y;Venugopal JR;El-Turki A;Ramakrishna S;Su B;Lim CT .Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering.[J].Biomaterials,2008(32):4314-4322. |
| [33] | Fujihara K;Kotaki M;Ramakrishna S .Guided bone regeneration membrane made of polycaprolactone/calcium carbonate composite nano-fibers[J].Biomaterials,2005(19):4139-4147. |
| [34] | Pirzada, T.;Arvidson, S.A.;Saquing, C.D.;Shah, S.S.;Khan, S.A. .Hybrid silica-PVA nanofibers via sol-gel electrospinning[J].Langmuir: The ACS Journal of Surfaces and Colloids,2012(13):5834-5844. |
| [35] | Schofer M D;Roessler P P;Schaefer J et al.Electrospun Plla Nanofiber Scaffolds and Their Use in Combination with Bmp-2 for Reconstruction of Bone Defects[J].PLoS One,2011,6(09):25462. |
| [36] | Woo,K.M.;Chen,V.J.;Jung,H.-M.;Kim,T.-I.;Shin,H.-I.;Baek,J.-H.;Ryoo,H.-M.;Ma,P.X. .Comparative evaluation of nanofibrous scaffolding for bone regeneration in critical-size calvarial defects[J].Tissue engineering, Part A,2009(8):2155-2162. |
| [37] | Liang D;Hsiao BS;Chu B .Functional electrospun nanofibrous scaffolds for biomedical applications.[J].Advanced drug delivery reviews,2007(14):1392-1412. |
| [38] | Zhang R;Ma PX .Poly(alpha-hydroxyl acids)/hydroxyapatite porous composites for bone-tissue engineering. I. Preparation and morphology.[J].Journal of biomedical materials research, Part B. Applied biomaterials,1999(4):446-455. |
| [39] | Nam YS;Park TG .Porous biodegradable polymeric scaffolds prepared by thermally induced phase separation.[J].Journal of biomedical materials research, Part B. Applied biomaterials,1999(1):8-17. |
| [40] | Smith LA;Ma PX .Nano-fibrous scaffolds for tissue engineering[J].Colloids and Surfaces, B. Biointerfaces,2004(3):125-131. |
| [41] | Peter X. Ma .Scaffolds for tissue fabrication[J].Materials Today,2004(5):30-39. |
| [42] | Smith, LA;Liu, XH;Ma, PX .Tissue engineering with nano-fibrous scaffolds[J].Soft matter,2008(11):2144-2149. |
| [43] | Ma PX;Choi JW .Biodegradable polymer scaffolds with well-defined interconnected spherical pore network.[J].Tissue engineering,2001(1):23-33. |
| [44] | Liu X;Ma PX .Phase separation, pore structure, and properties of nanofibrous gelatin scaffolds.[J].Biomaterials,2009(25):4094-4103. |
| [45] | Liu X;Ma PX .The nanofibrous architecture of poly(L-lactic acid)-based functional copolymers.[J].Biomaterials,2010(2):259-269. |
| [46] | Xiaohua Liu;Xiaobing Jin;Peter X. Ma .Nanofibrous hollow microspheres self-assembled from star-shaped polymers as injectable cell carriers for knee repair[J].Nature materials,2011(5):398-406. |
| [47] | Wei G;Ma PX .Macroporous and nanofibrous polymer scaffolds and polymer/bone-like apatite composite scaffolds generated by sugar spheres.[J].Journal of biomedical materials research, Part A,2006(2):306-315. |
| [48] | Lei B;Shin K H;Noh D Y et al.Nanofibrous Gelatin-Silica Hybrid Scaffolds Mimicking the Native Etracellular Matrix (Ecm) Using Thermally Induced Phase Separation[J].Jour-nal of Materials Chemistry,2012,22(28):14133-14140. |
| [49] | Liu X;Smith LA;Hu J;Ma PX .Biomimetic nanofibrous gelatin/apatite composite scaffolds for bone tissue engineering.[J].Biomaterials,2009(12):2252-2258. |
| [50] | Chuanglong He;Guiyong Xiao;Xiaobingjin;Chenghui Sun;Peter X. Ma .Electrodeposition on Nanofibrous Polymer Scaffolds: Rapid Mineralization, Tunable Calcium Phosphate Composition and Topography[J].Advanced functional materials,2010(20):3568-3576. |
| [51] | Chuanglong He;Fan Zhang;Lijun Cao .Rapid mineralization of porous gelatin scaffolds by electrodeposition for bone tissue engineering[J].Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology,2012(5):2111-2119. |
| [52] | Yoshimoto H;Shin YM;Terai H;Vacanti JP .A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering.[J].Biomaterials,2003(12):2077-2082. |
| [53] | Woo K M;Chen V J;Ma P X .Nano-Fibrous Scaffolding Archi-tecture Selectively Enhances Protein Adsorption Contributing to Cell Attachment[J].Journal of Biomedical Materials Research Part A,2003,67(02):531-537. |
| [54] | Binulal N;Deepthy M;Selvamurugan N et al.Role of Nanofi-brous Poly (Caprolactone) Scaffolds in Human Mesenchymal Stem Cell Attachment and Spreading for in Vitro Bone Tissue Engineering-Response to Osteogenic Regulators[J].Tissue En-gineering Part A,2010,16(02):393-404. |
| [55] | Wang J;Ma H;Jin X;Hu J;Liu X;Ni L;Ma PX .The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells.[J].Biomaterials,2011(31):7822-7830. |
| [56] | Seong, J.M.;Kim, B.-C.;Park, J.-H.;Kwon, I.K.;Mantalaris, A.;Hwang, Y.-S. .Stem cells in bone tissue engineering[J].Biomedical materials,2010(6):062001-1-062001-15. |
| [57] | Hu J;Feng K;Liu X .Chondrogenic and osteogenic differentiations of human bone marrow-derived mesenchymal stem cells on a nanofibrous scaffold with designed pore network.[J].Biomaterials,2009(28):5061-5067. |
| [58] | Xin XJ;Hussain M;Mao JJ .Continuing differentiation of human mesenchymal stem cells and induced chondrogenic and osteogenic lineages in electrospun PLGA nanofiber scaffold[J].Biomaterials,2007(26):316-325. |
| [59] | Smith LA;Liu X;Hu J;Ma PX .The influence of three-dimensional nanofibrous scaffolds on the osteogenic differentiation of embryonic stem cells.[J].Biomaterials,2009(13):2516-2522. |
| [60] | Kao CL;Tai LK;Chiou S .Resveratrol promotes osteogenic differentiation and protects against dexamethasone damage in murine induced pluripotent stem cells.[J].Stem cells and development,2010(2):247-258. |
| [61] | Wei G;Jin Q;Giannobile WV;Ma PX .The enhancement of osteogenesis by nano-fibrous scaffolds incorporating rhBMP-7 nanospheres.[J].Biomaterials,2007(12):2087-2096. |
| [62] | Cai,Y.Z.;Wang,L.L.;Cai,H.X.;Qi,Y.Y.;Zou,X.H.;Ouyang,H.W. .Electrospun nanofibrous matrix improves the regeneration of dense cortical bone[J].Journal of biomedical materials research, Part A,2010(1):49-57. |
| [63] | Woo,K.M.;Chen,V.J.;Jung,H.-M.;Kim,T.-I.;Shin,H.-I.;Baek,J.-H.;Ryoo,H.-M.;Ma,P.X. .Comparative evaluation of nanofibrous scaffolding for bone regeneration in critical-size calvarial defects[J].Tissue engineering, Part A,2009(8):2155-2162. |
| [64] | Liu,H.;Peng,H.;Wu,Y.;Zhang,C.;Cai,Y.;Xu,G.;Li,Q.;Chen,X.;Ji,J.;Zhang,Y.;OuYang,H.W. .The promotion of bone regeneration by nanofibrous hydroxyapatite/chitosan scaffolds by effects on integrin-BMP/Smad signaling pathway in BMSCs[J].Biomaterials,2013(18):4404-4417. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%