模似生物体组织的结构与状态、研制细胞与支架材料三维复合体,将有望开发出用于修复受损组织和器官的生物替代品.细胞的正常生物活动需要来自细胞外微环境中的信号分子持续刺激.作为人工细胞外基质的重要组成部分,材料的物理及化学信号能够影响细胞基因及蛋白表达,但远不足以达到体外重建组织器官的长远目标.利用基因与蛋白质工程技术对天然蛋白结构进行融合改造,设计并生物合成具有多重结构与功能的融合蛋白生物材料,可用于改善天然或化学合成生物材料的生物活性.综述了国内外基于融合蛋白的生物材料发展动态,概括了融合蛋白的设计思路与制备技术,重点详述了与细胞生长因子以及细胞粘附因子相关的融合蛋白在组织工程领域的应用.
参考文献
| [1] | Hench L L;Polak J M .Third-Generation Biomedical Materials[J].SCIENCE,2002,295(5557):1014-1017. |
| [2] | Humes H D .Tissue Engineering of a Bioartificial Kidney:a Universal Donor Organ[J].TRANSPLANTATION PROCEEDINGS,1996,28(4):2032-2035. |
| [3] | Langer R;Vacanti J P .Tissue Engineering[J].SCIENCE,1993,260(5110):920-926. |
| [4] | Morgan D. Mager;Vanessa LaPointe;Molly M. Stevens .Exploring and exploiting chemistry at the cell surface[J].Nature Chemistry,2011(8):582-589. |
| [5] | Michael Goldberg;Robert Langer;Xinqiao Jia .Nanostructured materials for applications in drug delivery and tissue engineering[J].Journal of biomaterials science,2007(3):241-268. |
| [6] | Meyer D E;Chilkoti A .Genetically Encoded Synthesis of Protein-Based Polymers with Precisely Specified Molecular Weight and Sequence by Recursive Directional Ligation:Examples from the Elastin-Like Polypeptide System[J].Biomacromolecules,2002,3(2):357-367. |
| [7] | Silvia Gomes;Isabel B. Leonor;Joao F. Mano .Natural and genetically engineered proteins for tissue engineering[J].Progress in Polymer Science,2012(1):1-17. |
| [8] | Daniel C. Anderson;Jason A. Burdick;Robert Langer .Smart Biomaterials[J].Science,2004(5692):1923-1924. |
| [9] | Lutolf MP;Hubbell JA .Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering[J].Nature biotechnology,2005(1):47-55. |
| [10] | Straley K;Heilshorn S.Designer Protein-Based Scaffolds for Neural Tissue Engineering[J].Conf Proc IEEE Eng Med Biol Soc,2009:2101-2102. |
| [11] | Sengupta D;Heilshorn SC .Protein-engineered biomaterials: highly tunable tissue engineering scaffolds.[J].Tissue engineering, Part B. Reviews,2010(3):285-293. |
| [12] | Sandberg L B;Leslie J G;Leach C T et al.Elastin Covalent Structure as Determined by Solid Phase Amino Acid Sequencing[J].Pathologie Biologie(Paris),1985,33(4):266-274. |
| [13] | Urry D W .Molecular Machines:How Motion and Other Functions of Living Organisms Can Result from Reversible Chemical Changes[J].Angew Chem Int Ed Engl,1993,32(6):819-841. |
| [14] | Gosline J M;DeMont M E;Denny M W .The Structure and Properties of Spider Silk[J].ENDEAVOUR,1986,10(1):37-43. |
| [15] | Nagarsekar A;Crissman J;Crissman M et al.Genetic Engineering of Stimuli-Sensitive Silkelastin-Like Protein Block Copolymers[J].Biomacromolecules,2003,4(3):602-607. |
| [16] | Masahiro Hayashi;Masahiro Tomita;Katsutoshi Yoshizato .Production of EGF-collagen chimeric protein which shows the mitogenic activity[J].Biochimica et Biophysica Acta. General Subjects,2001(2/3):187-195. |
| [17] | Kazutaka Ogiwara;Masato Nagaoka;Toshihiro Akaike;Chong-Su Cho .Construction of a novel extracellular matrix using a new genetically engineered epidermal growth factor fused to IgG-Fc[J].Biotechnology Letters,2005(20):1633-1637. |
| [18] | Zhang J;Ding L;Zhao Y;Sun W;Chen B;Lin H;Wang X;Zhang L;Xu B;Dai J .Collagen-targeting vascular endothelial growth factor improves cardiac performance after myocardial infarction.[J].Circulation: An Official Journal of the American Heart Association,2009(13):1776-1784. |
| [19] | Sun W J;Sun C K;Zhao H et al.Improvement of Sciatic Nerve Regeneration Using Laminin-Binding Human NGF-Beta[J].PLoS One,2009,4(7):e6180. |
| [20] | Czajkowsky D M;Hu J;Shao Z et al.Fc-Fusion Proteins:New Developments and Future Perspectives[J].EMBO Mol Med,2012,4(10):1015-1028. |
| [21] | Cherkupally P;Ramesh S;Torre B G .Immobilized Coupling Reagents:Synthesis of Amides/Peptides[J].ACS Comb Sci,2014,16(11):579-601. |
| [22] | Cai L;Heilshorn S C .Designing ECM-mimetic Materials Using Protein Engineering[J].ACTA BIOMATERIALIA,2014,10(4):1751-1760. |
| [23] | Clase Gustafsson;Sridhar Govindarajan;Jeremy Minshull .Codon bias and heterologous protein expression[J].Trends in Biotechnology,2004(7):346-353. |
| [24] | Welch M;Govindarajan S;Ness J E et al.Design Parameters to Control Synthetic Gene Expression in Escherichia Coli[J].PLoS One,2009,4(9):e7002. |
| [25] | Wurm FM .Production of recombinant protein therapeutics in cultivated mammalian cells[J].Nature biotechnology,2004(11):1393-1398. |
| [26] | Adams J C;Watt F M .Regulation of the Development and Differentiation by Extracellular Matrix[J].DEVELOPMENT,1993,117(4):1183-1198. |
| [27] | Werner S;Grose R .Regulation of wound healing by growth factors and cytokines.[J].Physiological Reviews,2003(3):835-870. |
| [28] | Whitaker MJ;Quirk RA;Howdle SM;Shakesheff KM .Growth factor release from tissue engineering scaffolds.[J].Journal of Pharmacy and Pharmacology,2001(11):1427-1437. |
| [29] | Tada S;Kitajima T;Ito Y .Design and Synthesis of Binding Growth Factors[J].Int JMol Sci,2012,13(5):6053-6072. |
| [30] | Cohen S .The Epidermal Growth Factor (EGF)[J].CANCER,1983,51(10):1787-1791. |
| [31] | Yang,X.;Kessler,E.;Su,L.-J.;Thorburn,A.;Frankel,A.E.;Li,Y.;LaRosa,F.G.;Shen,J.;Li,C.-Y.;Varella-Garcia,M.;Glodé,L.M.;Flaig,T.W. .Diphtheria toxin-epidermal growth factor fusion protein DAB 389EGF for the treatment of bladder cancer[J].Clinical cancer research: an official journal of the American Association for Cancer Research,2013(1):148-157. |
| [32] | Lan K L;Li J J;Tsai W C et al.Biological Evaluation of 111 InDTPA-VEGF-EGF Fusion Protein in a Human Breast Cancer Cells Expressing EGFR/VEGFR[J].Journal of Nuclear Medicine,2012,53(1):1719. |
| [33] | Squires C H;Childs J;Eisenberg S P et al.Production and Characterization of Human Basic Fibroblast Growth Factor from Escherichia Coli[J].Journal of Biological Chemistry,1988,263(31):16297-16302. |
| [34] | Hashi H;Hatai M;Kimizuka F et al.Angiogenic Activity of a Fusion Protein of the Cell-Binding Domain of Fibronectin and Basic Fibroblast Growth Factor[J].CELL STRUCTURE AND FUNCTION,1994,19(1):37-47. |
| [35] | Saito W;Uchida K;Ueno M et al.Acceleration of Bone Formation During Fracture Healing by Injectable Collagen Powder and Human Basic Fibroblast Growth Factor Containing a Collagen-Binding Domain from Clostridium Histolyticum Collagenase[J].Journal of Biomedical Materials Research Part A,2014,102(9):3049-3055. |
| [36] | Uchida,K.;Matsushita,O.;Naruse,K.;Mima,T.;Nishi,N.;Hattori,S.;Ogura,T.;Inoue,G.;Tanaka,K.;Takaso,M. .Acceleration of periosteal bone formation by human basic fibroblast growth factor containing a collagen-binding domain from Clostridium histolyticum collagenase[J].Journal of biomedical materials research, Part A,2014(6):1737-1743. |
| [37] | Lesko E;Majka M .The biological role of HGF-MET axis in tumor growth and development of metastasis.[J].Frontiers in bioscience: a journal and virtual library,2008(4):1271-1280. |
| [38] | Kitajima T;Terai H;Ito Y .A fusion protein of hepatocyte growth factor for immobilization to collagen.[J].Biomaterials,2007(11):1989-1997. |
| [39] | Ohkawara N;Ueda H;Shinozaki S;Kitajima T;Ito Y;Asaoka H;Kawakami A;Kaneko E;Shimokado K .Hepatocyte growth factor fusion protein having collagen-binding activity (CBD-HGF) accelerates re-endothelialization and intimal hyperplasia in balloon-injured rat carotid artery.[J].Journal of atherosclerosis and thrombosis,2007(4):185-191. |
| [40] | Azuma K;Nagaoka M;Cho C .An artificial extracellular matrix created by hepatocyte growth factor fused to IgG-Fc.[J].Biomaterials,2010(5):802-809. |
| [41] | Kelinheinz J;Jung S;Wermker K et al.Release Kinetics of VEGF165 from a Collagen Matrix and Structural Matrix Changes in a Circulation Model[J].Head & Face Medicine,2010,19,6:17. |
| [42] | Yu M H;Du F Y;Hirohiko I et al.Preparation and Characterization of a Fusion Protein Matrix VEGF-Fc for Enhancing HUVECs growth[J].BiotechnolLett,2012,34(9):1765-1771. |
| [43] | 于美华,杜凤移,饶霞,姚芳莲,杨军.人工细胞外基质对血管内皮细胞生存的影响[J].高等学校化学学报,2013(03):746-750. |
| [44] | Zhu C S;Xu J B;Yu M H et al.The Immobilization of VEGF-Fc for Promoting Endothelial Cells Proliferation in Porous PCL Scaffolds.Advanced Materials Research[J].Advanced Materials Research,2012,554:1721-1724. |
| [45] | Rosso F;Giordano A;Barbarisi M;Barbarisi A .From Cell-ECM interactions to tissue engineering.[J].Journal of Cellular Physiology,2004(2):174-180. |
| [46] | Edelman G M;Crossin K L .Cell Adhesion Molecules:Implications for a Molecular Histology[J].Annual Review of Biochemistry,1991,60(1):155-190. |
| [47] | Xavier Punet;Rodolphe Mauchauffe;Marina I. Giannotti .Enhanced Cell-Material Interactions through the Biofunctionalization of Polymeric Surfaces with Engineered Peptides[J].Biomacromolecules,2013(8):2690-2702. |
| [48] | Thiery, Jean Paul;Engl, Wilfried;Viasnoff, Virgile;Dufour, Sylvie .Biochemical and biophysical origins of cadherin selectivity and adhesion strength[J].Current Opinion in Cell Biology,2012(5):614-619. |
| [49] | Nelson WJ .Regulation of cell-cell adhesion by the cadherin-catenin complex.[J].Biochemical Society Transactions,2008(2):149-155. |
| [50] | Maltre J L;Heisenberg C P .Three Functions of Cadherins in Cell Adhesion[J].CURRENT BIOLOGY,2013,23(14):R626-R633. |
| [51] | Nagaoka M;Ise H;Akaike T .Immobilized E-cadherin model can enhance cell attachment and differentiation of primary hepatocytes but not proliferation[J].Biotechnology Letters,2002(22):1857-1862. |
| [52] | Drees F;Reilein A;Nelson W J .Cell-Adhesion Asays:Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Membrane Patches[J].Methods in Molecular Biology,2005,294:303-320. |
| [53] | Johana C. M. Vega L.;Min Kyung Lee;Jae Hyun Jeong .Recapitulating Cell-Cell Adhesion Using N-Cadherin Biologically Tethered to Substrates[J].Biomacromolecules,2014(6):2172-2179. |
| [54] | Kamiya K;Tsumoto K;Yoshimura T;Akiyoshi K .Cadherin-integrated liposomes with potential application in a drug delivery system.[J].Biomaterials,2011(36):9899-9907. |
| [55] | Behjati,M.;Kazemi,M.;Hashemi,M.;Zarkesh-Esfahanai,S.H.;Bahrami,E.;Hashemi-Beni,B.;Ahmadi,R. .Double-chimera proteins to enhance recruitment of endothelial cells and their progenitor cells[J].International Journal of Cardiology,2013(4):1560-1569. |
| [56] | Collazos-Castro,J.E.;Hern谩ndez-Labrado,G.R.;Polo,J.L.;Garc铆a-Rama,C. .N-Cadherin- and L1-functionalised conducting polymers for synergistic stimulation and guidance of neural cell growth[J].Biomaterials,2013(14):3603-3617. |
| [57] | Elsie S. Place;Nicholas D. Evans;Molly M. Stevens .Complexity in biomaterials for tissue engineering[J].Nature Materials,2009(6):457-470. |
| [58] | Bian, L.;Guvendiren, M.;Mauck, R.L.;Burdick, J.A. .Hydrogels that mimic developmentally relevant matrix and N-cadherin interactions enhance MSC chondrogenesis[J].Proceedings of the National Academy of Sciences of the United States of America,2013(25):10117-10122. |
| [59] | Zhang Y;Xiang Q;Dong S;Li C;Zhou Y .Fabrication and characterization of a recombinant fibronectin/cadherin bio-inspired ceramic surface and its influence on adhesion and ossification in vitro.[J].Acta biomaterialia,2010(3):776-785. |
| [60] | Zhang,Y.;Li,L.;Zhu,J.;Kuang,H.;Dong,S.;Wang,H.;Zhang,X.;Zhou,Y. .In vitro observations of self-assembled ECM-mimetic bioceramic nanoreservoir delivering rFN/CDH to modulate osteogenesis[J].Biomaterials,2012(30):7468-7477. |
| [61] | Haque A;Hexig B;Meng Q;Hossain S;Nagaoka M;Akaike T .The effect of recombinant E-cadherin substratum on the differentiation of endoderm-derived hepatocyte-like cells from embryonic stem cells.[J].Biomaterials,2011(8):2032-2042. |
| [62] | Haque,A.;Yue,X.-S.;Motazedian,A.;Tagawa,Y.-I.;Akaike,T. .Characterization and neural differentiation of mouse embryonic and induced pluripotent stem cells on cadherin-based substrata[J].Biomaterials,2012(20):5094-5106. |
| [63] | Elnakish MT;Hassan F;Dakhlallah D.Mesenchymal Stem Cells for Cardiac Regeneration:Translation to Bedside Reality[J].Stem Cells Int,2012:646038. |
| [64] | Wagner W;Horn P;Castoldi M et al.Replicative Senescence of Mesenchymal Stem Cells a Continuous and Organized Process[J].PLoS One,2008,3(5):e2213. |
| [65] | LIOR ZANGI;RACHEL RIVKIN;IBRAHIM KASSIS;LEVDANSKY .High-Yield Isolation, Expansion, and Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells with Fibrin Microbeads[J].Tissue engineering,2006(8):2343-2354. |
| [66] | Zhong,S.;Zhang,Y.;Lim,C.T. .Fabrication of large pores in electrospun nanofibrous scaffolds for cellular infiltration: A review[J].Tissue engineering, Part B. Reviews,2012(2):77-87. |
| [67] | Xu, J.;Zhu, C.;Zhang, Y.;Jiang, N.;Li, S.;Su, Z.;Akaike, T.;Yang, J..HE-cadherin-Fc fusion protein coated surface enhances the adhesion and proliferation of human mesenchymal stem cells[J].Colloids and Surfaces, B. Biointerfaces,2013:97-102. |
| [68] | Jianbin Xu;Suhua Li;Feifei Hu;Chuanshun Zhu;Yan Zhang;Wei Zhao;Toshihiro Akaike;Jun Yang .Artificial Biomimicking Matrix Modifications of Nanofibrous Scaffolds by hE-Cadherin-Fc Fusion Protein to Promote Human Mesenchymal Stem Cells Adhesion and Proliferation[J].Journal of nanoscience and nanotechnology,2014(6):4007-4013. |
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