利用紫外-可见吸收光谱、荧光光谱、圆二色谱(CD)等方法研究了色胺修饰竹红菌素(DTrpHA)及其稀土离子配位聚合物(y3+-DTrpHA,La3+-DTrpHA)与小牛胸腺DNA (CT DNA)和G-四链体22AG的相互作用.结果表明,DTrpHA及其配位聚合物中的色胺基团和竹红菌素基团均参与和双链CTDNA的作用,作用方式主要为沟槽作用.与G-四链体DNA作用后,DTrpHA及其配位聚合物中的色胺基团均具有较大的减色效应(＞45％)和峰位红移(≥4 nm),说明色胺基团与G-四链体采用外部堆积作用方式结合;而竹红菌素基团的减色效应相对较小且无明显峰位变化,表明竹红菌素基团采用非特异性作用方式与G-四链体的环区碱基或糖-磷酸骨架结合.G-四链体22AG的构象主要为分子内反平行结构,加入DTrpHA及其配位聚合物对G-四链体22AG的构象影响较小.Y3+-DTrpHA比DTrpHA和La3+-DTrpHA与G-四链体具有更强的相互作用.Y3+-DTrpHA使得CT DNA的熔解温度(Tm)上升了仅1.9℃,而使G-四链体的熔解温度上升了13.1℃.荧光嵌插剂置换实验(FID)结果表明,y3+-DTrpHA对G-四链体具有良好亲和性,具有较小的G4 DC50值(使噻唑橙/G-四链体体系荧光下降50％所需配体或配合物的浓度)和较高的G四链体选择性.
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