欢迎登录材料期刊网

材料期刊网

高级检索

通过紫外-可见光谱、荧光光谱、同步荧光光谱、圆二色谱、衰减全反射红外光谱、负染-透射电镜、等温滴定微量热等实验方法系统地探讨了咪唑型离子液体与牛血清蛋白(BSA)的缔合特性.结果发现,离子液体[Bmim]Cl的加入使得BSA的紫外吸收强度增加,同时也会导致其荧光猝灭,并且这种猝灭是静态猝灭.同步荧光的研究结果表明,[Bmim] Cl分子可与蛋白质中接近色氨酸残基的区域发生相互作用,使蛋白质的构象和内部的疏水结构发生改变;负染色法透射电镜直观地显示了加入离子液体后形成的蛋白质-离子液体复合物结构逐渐变大;圆二色谱和衰减全反射红外光谱表明:在离子液体与BSA缔合过程中,离子液体的加入使得BSA二级结构中的α-螺旋和β-折叠的含量降低,从而引起蛋白质二级结构的变化;表面张力法和等温滴定微量热法进一步证实上述缔合作用为静电作用和疏水作用共同作用的结果,但离子液体的烷基链与BSA疏水内腔之间的疏水作用是离子液体与BSA缔合的主要驱动力.

参考文献

[1] Jonathan G. Huddleston;Ann E. Visser;W. Matthew Reichert .Characterization and comparison of hydrophilic and hydrophobic room temperature ionic liquids incorporating the imidazolium cation[J].Green chemistry,2001(4):156-164.
[2] He YY;Li ZB;Simone P;Lodge TP .Self-assembly of block copolymer micelles in an ionic liquid[J].Journal of the American Chemical Society,2006(8):2745-2750.
[3] Seth D;Chakraborty A;Setua P;Sarkar N .Interaction of ionic liquid with water in ternary microemulsions (Triton X-100/Water/1-butyl-3 methylimidazolium hexafluoro-phosphate) probed by solvent and rotational relaxation of coumarin 153 and coumarin 151[J].LANGMUIR,2006,22(18):7768-7775.
[4] Welton T .Room-temperature ionic liquids.solvents for synthesis and catalysis[J].Chemical Reviews,1999,99(8):2071-2084.
[5] Avery T D;Jenkins N F;Kimber M C;Lupton D W Taylor D K .First examples of the catalytic asymmetric ring-opening of meso 1,2-dioxines utilising cobalt(Ⅱ) complexes with optically active tetradentate schiff base ligands:formation of enantio-enriched cyclopropanes[J].Chemical Communications,2002(1):28-29.
[6] Jonathan G. Huddleston;Heather D. Willauer;Richard P. Swatloski;Ann E. Visser;Robin D. Rogers .Room temperature ionic liquids as novel media for 'clean' liquid-liquid extraction[J].Chemical communications,1998(16):1765-1766.
[7] Michael Smietana;Charies Mioskowski .Preparation of Silyl Enol Ethers Using (Bistrimethylsilyl)acetamide in Ionic Liquids[J].Organic letters,2001(7):1037-1039.
[8] Kenta Fukumoto;Masahiro Yoshizawa;Hiroyuki Ohno .Room Temperature Ionic Liquids from 20 Natural Amino Acids[J].Journal of the American Chemical Society,2005(8):2398-2399.
[9] Anthony M.Leone;Stephanie C.Weatherly;Mary Elizabeth Williams;H.Holden Thorp;Royce W.Murray .An Ionic Liquid Form of DNA: Redox-Active Molten Salts of Nucleic Acids[J].Journal of the American Chemical Society,2001(2):218-222.
[10] Peter Wasserscheid;Wilhelm Keim .Ionic Liquids-New "Solutions"for Transition Metal Catalysis[J].Angewandte Chemie International Edition,2000(21):3773-3789.
[11] Anderson JL.;Ding J.;Welton T.;Armstrong DW. .Characterizing ionic liquids on the basis of multiple solvation interactions[J].Journal of the American Chemical Society,2002(47):14247-14254.
[12] Chun S;Dzyuba S V;Bartsch R A .Influence of structural variation in room-temperature ionic liquids on the selectivity and efficiency of competitive alkai metal salt extraction by a crown ether[J].Analytical Chemistry,2001,73(15):3737-3741.
[13] Lynnette A. Blanchard;Joan F. Brennecke .Recovery of Organic Products from Ionic Liquids Using Supercritical Carbon Dioxide[J].Industrial & Engineering Chemistry Research,2001(1):287-292.
[14] Ki-Sub Kim;D.Demberelnyamba;Huen Lee .Size-Selective Synthesis of Gold and Platinum Nanoparticles Using Novel Thiol-Functionalized Ionic Liquids[J].Langmuir: The ACS Journal of Surfaces and Colloids,2004(3):556-560.
[15] Yong Zhou;Markus Antonietti .Synthesis of Very Small TiO_2 Nanocrystals in a Room-Temperature Ionic Liquid and Their Self-Assembly toward Mesoporous Spherical Aggregates[J].Journal of the American Chemical Society,2003(49):14960-14961.
[16] Andreas Taubert .CuCl Nanoplatelets from an Ionic Liquid-Crystal Precursor[J].Angewandte Chemie,2004(40):5380-5382.
[17] Torsten Brezesinski;Christian Erpen;Ken-ichi limura;Bernd Smarsly .Mesostructured Crystalline Ceria with a Bimodal Pore System Using Block Copolymers and Ionic Liquids as Rational Templates[J].Chemistry of Materials,2005(7):1683-1690.
[18] Scheeren CW.;Machado G.;Dupont J.;Fichtner PFP.;Texeira SR. .Nanoscale Pt(0) particles prepared in imidazolium room temperature ionic liquids: Synthesis from an organometallic precursor, characterization, and catalytic properties in hydrogenation reactions[J].Inorganic Chemistry: A Research Journal that Includes Bioinorganic, Catalytic, Organometallic, Solid-State, and Synthetic Chemistry and Reaction Dynamics,2003(15):4738-4742.
[19] Nishimura N.;Ohno H. .Design of successive ion conduction paths in DNA films with ionic liquids[J].Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology,2002(8):2299-2304.
[20] Nishimura N;Nomura Y;Nakamura N;Ohno H .DNA strands robed with ionic liquid moiety[J].Biomaterials,2005(27):5558-5563.
[21] Qin WD.;Li SFY. .Electrophoresis of DNA in ionic liquid coated capillary[J].The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry,2003(1):37-41.
[22] Wang, JH;Cheng, DH;Chen, XW;Du, Z;Fang, ZL .Direct extraction of double-stranded DNA into ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate and its quantification[J].Analytical Chemistry,2007(26):620-625.
[23] Shimojo K;Kamiya N;Tani F;Naganawa H;Naruta Y;Goto M .Extractive solubilization, structural change, and functional conversion of cytochrome c in ionic liquids via crown ether complexation[J].Analytical chemistry,2006(22):7735-7742.
[24] Mozhgan Bagheri;Hector Rodrfguez;Richard P.Swatloski .Ionic Liquid-Based Preparation of Cellulose-Dendrimer Films as Solid Supports for Enzyme Immobilization[J].Biomacromolecules,2008(1):381-387.
[25] Kojiro Shimojo;Kazunori Nakashima;Noriho Kamiya;Masahiro Goto .Crown Ether-Mediated Extraction and Functional Conversion of Cytochrome c in Ionic Liquids[J].Biomacromolecules,2006(1):2-5.
[26] David M. Phillips;Lawrence F. Drummy;Deborah G. Conrady;Douglas M. Fox;Rajesh R. Naik;Morley O. Stone;Paul C. Trulove;Hugh C. De Long;Robert A. Mantz .Dissolution and Regeneration of Bombyx mori Silk Fibroin Using Ionic Liquids[J].Journal of the American Chemical Society,2004(44):14350-14351.
[27] Wang SF;Chen T;Zhang ZL;Shen XC;Lu ZX;Pang DW;Wong KY .Direct electrochemistry and electrocatalysis of heme proteins entrapped in agarose hydrogel films in room-temperature ionic liquids[J].Langmuir: The ACS Journal of Surfaces and Colloids,2005(20):9260-9266.
[28] Xianbo Lu;Jianqiang Hu;Xin Yao;Zhouping Wang;Jinghong Li .Composite System Based on Chitosan and Room-Temperature Ionic Liquid:Direct Electrochemistry and Electrocatalysis of Hemoglobin[J].Biomacromolecules,2006(3):975-980.
[29] Wang SF;Chen T;Zhang ZL;Pang DW .Activity and stability of horseradish peroxidase in hydrophilic room temperature ionic liquid and its application in non-aqueous biosensing[J].Electrochemistry communications,2007(6):1337-1342.
[30] Ya-Ni Xie;Sheng-Fu Wang;Zhi-Ling Zhang .Interaction between Room Temperature Ionic Liquid[bmim]BF4 and DNA Investigated by Electrochemical Micromethod[J].The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical,2008(32):9864-9868.
[31] Tanford C.The Hydrophobic Effect:Formation of Micelles and Biological Membranes[M].New York:Wiley-Interscience,1980
[32] Yuanhua Ding;Ying Shu;Lingling Ge;Rong Guo .The effect of sodium dodecyl sulfate on the conformation of bovine serum albumin[J].Colloids and Surfaces, A. Physicochemical and Engineering Aspects,2007(3):163-169.
[33] Rajan Patel;Meena Kumari;Abbul Bashar Khan .Recent Advances in the Applications of Ionic Liquids in Protein Stability and Activity: A Review[J].Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology,2014(8):3701-3720.
[34] Diana Constatinescu;Christian Herrmann;Hermann Weingartner .Patterns of protein unfolding and protein aggregation in ionic liquids[J].Physical chemistry chemical physics: PCCP,2010(8):1756-1763.
[35] Hermann Weingartner;Chiara Cabrele;Christian Herrmann .How ionic liquids can help to stabilize native proteins[J].Physical chemistry chemical physics: PCCP,2012(2):415-426.
[36] Tejwant Singh;Shilpi Boral;H. B. Bohidar .Interaction of Gelatin with Room Temperature Ionic Liquids: A Detailed Physicochemical Study[J].The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical,2010(25):8441-8448.
[37] Geng F;Zheng L;Yu L;Li G Tung C .Interaction of bovine serum albumin and long-chain imidazolium ionic liquid measured by fluorescence spectra and surface tension[J].Process Biochemistry,2010,45(3):306?311.
[38] Yan, H.;Wu, J.;Dai, G.;Zhong, A.;Chen, H.;Yang, J.;Han, D. .Interaction mechanisms of ionic liquids [C _nmim]Br (n=4, 6, 8, 10) with bovine serum albumin[J].Journal of Luminescence: An Interdisciplinary Journal of Research on Excited State Processes in Condensed Matter,2012(3):622-628.
[39] Xiaoqing Wang;Jie Liu;Limei Sun .Interaction of Bovine Serum Albumin with Ester-Functionalized Anionic Surface-Active Ionic Liquids in Aqueous Solution: A Detailed Physicochemical and Conformational Study[J].The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical,2012(41):12479-12488.
[40] Peter Wasserscheid;Wilhelm Keim .Ionic Liquids-New "Solutions"for Transition Metal Catalysis[J].Angewandte Chemie International Edition,2000(21):3773-3789.
[41] Baltazar QQ;Chandawalla J;Sawyer K;Anderson JL .Interfacial and micellar properties of imidazolium-based monocationic and dicationic ionic liquids[J].Colloids and Surfaces, A. Physicochemical and Engineering Aspects,2007(1/3):150-156.
[42] Urszula Domanska;Ewa Bogel-Lukasik;RafalBogel-Lukasik .1-Octanol/Water Partition Coefficients of 1-Alkyl-3-methylimidazolium Chloride~+[J].Chemistry: A European journal,2003(13):3033-3041.
[43] 陶慰孙;李惟;姜涌明.蛋白质分子基础[M].北京:高等教育出版社,1995
[44] Wu Y .Study on the interaction between salicylic acid and catalase by spectroscopic methods.[J].Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis,2007(3):796-801.
[45] Zhou N;Liang YZ;Wang P .Characterization of the interaction between furosemide and bovine serum albumin[J].Journal of Molecular Structure,2008(2/3):190-196.
[46] Lakowicz J R.Principles of Fluorescence Spectroscopy[M].New York:Plenum Press,1983
[47] 黄波,邹国林,杨天鸣.阿霉素与牛血清白蛋白结合作用的研究[J].化学学报,2002(10):1867-1871.
[48] Shang L;Wang YZ;Jiang JG;Dong SJ .pH-dependent protein conformational changes in albumin : gold nanoparticle bioconjugates: A spectroscopic study[J].Langmuir: The ACS Journal of Surfaces and Colloids,2007(5):2714-2721.
[49] Antonios Kelarakis;Valeria Castelletto;Marta J. Krysmann .Interactions of Bovine Serum Albumin with Ethylene Oxide/ Butylene Oxide Copolymers in Aqueous Solution[J].Biomacromolecules,2008(5):1366-1371.
[50] Zheng L;Guo C;Wang J;Liang XF;Chen S;Ma JH;Yang B;Jiang YY;Liu HZ .Effect of ionic liquids on the aggregation behavior of PEO-PPO-PEO block copolymers in aqueous solution[J].The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical,2007(6):1327-1333.
[51] Rungsardthong U;Ehtezazi T;Bailey L;Armes S P Garnett M C Stolnik S .Effect of polymer ionization on the interaction with DNA in nonviral gene delivery systems[J].Biomacromolecules,2003,4(3):683-690.
[52] Hrushikesh Joshi;Pravin S.Shirude;Vipul Bansal;K.N.Ganesh;Murali Sastry .Isothermal Titration Calorimetry Studies on the Binding of Amino Acids to Gold Nanoparticles[J].The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical,2004(31):11535-11540.
上一张 下一张
上一张 下一张
计量
  • 下载量()
  • 访问量()
文章评分
  • 您的评分:
  • 1
    0%
  • 2
    0%
  • 3
    0%
  • 4
    0%
  • 5
    0%