以L-半胱氨酸(L-Cys)和巯基丙酸(MPA)为共修饰剂在水相中快速合成高质量CdTe量子点.通过紫外-可见分光光谱、荧光光谱、荧光寿命衰减曲线、透射电子显微图片、XRD图谱等相关方法对产物进行表征.调节L-Cys和MPA的摩尔比,对CdTe量子点的生长速率和光学特性有明显影响.结果证实:与单一修饰剂L-Cys或MPA相比,L-Cys/MPA共修饰CdTe量子点具有较快的荧光发射峰红移速率,且其粒径分布均一、稳定性强.50L-CdTe(L-Cys与MPA摩尔比为50％)的荧光量子产率达到66.4％,75L-CdTe(L-Cys与MPA摩尔比为75％)的荧光寿命为46.8 ns.细胞毒性实验证明75L-CdTe量子点对SiHa细胞毒性较小,细胞存活率为75％～95％.进一步将其用于标记细胞,表明75L-CdTe量子点能有效地对SiHa细胞进行荧光标记.L-Cys/MPA共修饰CdTe量子点具有良好的荧光特性和生物相容性,在生物医药领域具有重要的应用价值.
参考文献
| [1] | Medintz IL;Uyeda HT;Goldman ER;Mattoussi H .Quantum dot bioconjugates for imaging, labelling and sensing[J].Nature materials,2005(6):435-446. |
| [2] | Schlamp MC.;Alivisatos AP.;Peng XG. .IMPROVED EFFICIENCIES IN LIGHT EMITTING DIODES MADE WITH CDSE(CDS) CORE/SHELL TYPE NANOCRYSTALS AND A SEMICONDUCTING POLYMER[J].Journal of Applied Physics,1997(11):5837-5842. |
| [3] | Mattoussi H.;Dabbousi BO.;Thomas EL.;Bawendi MG. Rubner MF.;Radzilowski LH. .Electroluminescence from heterostructures of poly(phenylene vinylene) and inorganic CdSe nanocrystals[J].Journal of Applied Physics,1998(12):7965-7974. |
| [4] | James B. Delehanty;Kimihiro Susumu;Rachel L. Manthe.Active cellular sensing with quantum dots: Transitioning from research tool to reality; a review[J].Analytica chimica acta,2012:63-81. |
| [5] | Murray C B;Norris D J;Bawendi M G .Synthesis and characterization of nearly monodisperse CdE (E=S,Se,Te)semiconductor nanocrystallites[J].Journal of the American Chemical Society,1993,115(19):8706-8715. |
| [6] | Smith AM;Duan H;Mohs AM;Nie S .Bioconjugated quantum dots for in vivo molecular and cellular imaging.[J].Advanced drug delivery reviews,2008(11):1226-1240. |
| [7] | CaiWB;HsuAR;LiZB;ChenXY .Are quantum dots ready for in vivo imaging in human subjects[J].Nanoscale Research Letters,2007,2(06):265-281. |
| [8] | Yuan, Z.;Zhang, A.;Cao, Y.;Yang, J.;Zhu, Y.;Yang, P. .Effect of mercaptocarboxylic acids on luminescent properties of CdTe quantum dots[J].Journal of Fluorescence,2012(1):121-127. |
| [9] | Blum AS;Moore MH;Ratna BR .Quantum dot fluorescence as a function of alkyl chain length in aqueous environments[J].Langmuir: The ACS Journal of Surfaces and Colloids,2008(17):9194-9197. |
| [10] | Gaponik N;Talapin D V;Rogaeh A L;Hoppe K Schevchenko E V Kornowski A Eychmuller A Weller H .Thiol-capping of CdTe nanoerystals:An alternative to organometallic synthetic routes[J].Journal of Physical Chemistry B,2002,106(29):7177-7185. |
| [11] | SasakiMK;RibeiroDSM;FrigerioC;PriorJAV Santos J L M Zagatto E A G .Chemiluminometric determination of ascorbic acid in pharmaceutical formulations exploiting photo-activation of GSH-capped CdTe quantum dots[J].LUMINESCENCE,2014,29(07):901-907. |
| [12] | 董微,王莹,宋有涛,徐淑坤,葛欣.谷胱甘肽修饰的CdTe量子点共振光散射法测定溶菌酶及其分析应用[J].化学试剂,2012(12):1093-1095. |
| [13] | Mamedova N N;Kotov N A;Rogach A L;Studer J .Albumin-CdTe nanoparticle bioconjugates:preparation,structure,and interunit energy transfer with antenna effect[J].NANO LETTERS,2001,1(06):281-286. |
| [14] | Liu Y;Fei X N;Wang Y Q;Wang J .Preparation of CdTe/CdS core/shell quantum dots modified by chitosan and its spectral characteristics[J].Chalcogenide Letters,2011,8(02):111-115. |
| [15] | Markus Grabolle;Peter Kapusta;Thomas Nann;Xu Shu;Jan Ziegler;Ute Resch-Genger .Fluorescence Lifetime Multiplexing with Nanocrystals and Organic Labels[J].Analytical chemistry,2009(18):7807-7813. |
| [16] | Tan Fang;Kaiguo Ma;Lili Ma .3-Mercaptobutyric Acid as an Effective Capping Agent for Highly Luminescent CdTe Quantum Dots: New Insight into the Selection of Mercapto Acids[J].The journal of physical chemistry, C. Nanomaterials and interfaces,2012(22):12346-12352. |
| [17] | Hasselbarth A;Eychmuller A;Eichburger R;Giersig M Mews A Weller H .Chemistry and photophysics of mixed cadmium sulfide/mercury sulfide colloids[J].Journal of Physical Chemistry,1993,97(20):5333-5340. |
| [18] | Gaponik N;Talapin D V;Rogach A L;Hoppe K Shevchenko E V Kornowski A Eychmuller A Weller H .Thiol-capping of CdTe nanocrystals:an alternative to organometallic synthetic routes[J].Journal of Physical Chemistry B,2002,106:7177-7185. |
| [19] | Liu, N.;Yang, P. .Highly luminescent hybrid SiO_2-coated CdTe quantum dots: Synthesis and properties[J].Luminescence: The journal of biological and chemical luminescence,2013(4):542-550. |
| [20] | 梁佳然,钟文英,于俊生.高质量CdTe量子点的水相快速合成[J].高等学校化学学报,2009(01):14-18. |
| [21] | 石鹏.水溶性CdTe纳米晶的制备和发光性质的研究[J].光谱实验室,2008(03):390-393. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%