本文简单介绍了原子力显微镜的发展史,以及原子力显微镜的工作原理、工作模式、活细胞在生理状态下的成像方式等,特别介绍了生物型原子力显微镜、高速原子力显微镜在生物学领域的研究及应用.原子力显微镜在扫描速度、扫描范围、扫描精度方面的不断改进将为肿瘤细胞学研究提供源源不断的动力.本文着重阐述了原子力显微术在肿瘤领域的研究进展,包括原子力显微镜在肿瘤细胞形貌学特性、硬度、粘弹性方面的研究现状,并对原子力显微镜在肿瘤诊断及抗肿瘤药物研发方面的应用前景进行了展望.
参考文献
| [1] | WU Hui;I Meng;UN MengXiang.Atomic force microscopic view of the fine topography on the tobacco stigma surface during its response to pollination[J].科学通报（英文版）,2008(7):1015-1020. |
| [2] | Bonfiglio A;Parodi MT;Tonini GP.Subcellular details of early events of differentiation induced by retinoic acid in human neuroblastoma cells detected by atomic force microscope.[J].Experimental Cell Research,19951(1):73-79. |
| [3] | Jung SH;Park D;Park JH;Kim YM;Ha KS.Molecular imaging of membrane proteins and microfilaments using atomic force microscopy.[J].Experimental & molecular medicine,20109(9):597-605. |
| [4] | Sullan,R.M.A.;Churnside,A.B.;Nguyen,D.M.;Bull,M.S.;Perkins,T.T..Atomic force microscopy with sub-picoNewton force stability for biological applications[J].Methods: A Companion to Methods in Enzymology,20132(2):131-141. |
| [5] | Formosa, Cecile;Pillet, Flavien;Schiavone, Marion;Duval, Raphael E.;Ressier, Laurence;Dague, Etienne.Generation of living cell arrays for atomic force microscopy studies[J].Nature protocols erecipes for researchers,20151(1):199-215. |
| [6] | Youdong Mao;Quanmei Sun;Xiufeng Wang;Qi Ouyang;Li Han;Lei Jiang;Dong Han.In vivo nanomechanical imaging of blood-vessel tissues directly in living mammals using atomic force microscopy[J].Applied Physics Letters,20091(1):013704-1--013704-3-0. |
| [7] | CHEN PeiPei;DONG HongTao;CHEN Long;SUN QuanMei;HAN Dong.Application of atomic force microscopy to living samples from cells to fresh tissues[J].科学通报（英文版）,2009(14):2410-2415. |
| [8] | 李密;刘连庆;席宁;王越超.AFM单分子力谱技术测量膜蛋白力学特性的研究进展[J].科学通报,2014(13):1198-1208. |
| [9] | Emad Moeendarbary;Leo Valon;Marco Fritzsche.The cytoplasm of living cells behaves as a poroelastic material[J].Nature materials,20133(3):253-261. |
| [10] | Paloczi GT.;Hansma PK.;Walters DA.;Wendman MA.;Smith BL..Rapid imaging of calcite crystal growth using atomic force microscopy with small cantilevers[J].Applied physics letters,199812(12):1658-1660. |
| [11] | Mime Kobayashi;Koji Sumitomo;Keiichi Torimitsu.Real-time imaging of DNA-streptavidin complex formation in solution using a high-speed atomic force microscope[J].Ultramicroscopy,20072/3(2/3):184-190. |
| [12] | Yanyan Wang;Xiaodong Hu;Linyan Xu.Improving the scanning speed of atomic force microscopy at the scanning range of several tens of micrometers[J].Ultramicroscopy,2013:102-107. |
| [13] | Jing Li;Zhifeng Deng;Daixie Chen.High-speed AFM for scanning the architecture of living cells[J].Nanoscale,201318(18):8355-8358. |
| [14] | Sannohe, Y.;Endo, M.;Katsuda, Y.;Hidaka, K.;Sugiyama, H..Visualization of dynamic conformational switching of the G-quadruplex in a DNA nanostructure[J].Journal of the American Chemical Society,201046(46):16311-16313. |
| [15] | Kiyohiko lgarashi;Takayuki Uchihashi;Anu Koivula;Masahisa Wada;Satoshi Kimura;Tetsuaki Okamoto;Merja Penttila;Toshio Ando;Masahiro Samejima.Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface[J].Science,2011Sep.2 TN.6047(Sep.2 TN.6047):1279-1282. |
| [16] | Felix Rico;Laura Gonzalez;Ignacio Casuso;Manel Puig-Vidal;Simon Scheming.High-Speed Force Spectroscopy Unfolds Titin at the Velocity of Molecular Dynamics Simulations[J].Science,2013Nov.8 TN.6159(Nov.8 TN.6159):741-743. |
| [17] | Kilian, KA;Bugarija, B;Lahn, BT;Mrksich, M.Geometric cues for directing the differentiation of mesenchymal stem cells[J].Proceedings of the National Academy of Sciences of the United States of America,201011(11):4872-4877. |
| [18] | BIN CHEN;QI WANG;LI HAN.Using the Atomic Force Microscope to Observe and Study the Ultrastructure of the Living BIU-87 Cells of the Human Bladder Cancer[J].Scanning,20044(4):162-166. |
| [19] | 关维;纪小龙;范丽娜.原子力显微镜对正常细胞、肿瘤细胞细胞膜表面形态结构的观察[J].中国实验诊断学,2006(9):951-954,封3. |
| [20] | Mi LI;Lian-qing LIU;Ning XI;Yue-chao WANG.Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy[J].中国药理学报（英文版）,2015(7):769-782. |
| [21] | Fraley SI;Feng Y;Krishnamurthy R;Kim DH;Celedon A;Longmore GD;Wirtz D.A distinctive role for focal adhesion proteins in three-dimensional cell motility.[J].Nature cell biology,20106(6):598-604. |
| [22] | Cross SE;Jin YS;Rao J;Gimzewski JK.Nanomechanical analysis of cells from cancer patients[J].Nature nanotechnology,200712(12):780-783. |
| [23] | Li QS;Lee GY;Ong CN;Lim CT.AFM indentation study of breast cancer cells.[J].Biochemical and Biophysical Research Communications,20084(4):609-613. |
| [24] | Xiaofang Cai;Xiaobo Xing;Jiye Cai;Qian Chen;Shixian Wu;Feicheng Huang.Connection between biomechanics and cytoskeleton structure of lymphocyte and Jurkat cells: An AFM study[J].Micron: The international research and review journal for microscopy,20103(3):257-262. |
| [25] | Plodinec, M.;Loparic, M.;Monnier, C.A.;Obermann, E.C.;Zanetti-Dallenbach, R.;Oertle, P.;Hyotyla, J.T.;Aebi, U.;Bentires-Alj, M.;Lim, R.Y.H.;Schoenenberger, C.-A..The nanomechanical signature of breast cancer[J].Nature nanotechnology,201211(11):757-765. |
| [26] | Efremov,Y.M.;Lomakina,M.E.;Bagrov,D.V.;Makhnovskiy,P.I.;Alexandrova,A.Y.;Kirpichnikov,M.P.;Shaitan,K.V..Mechanical properties of fibroblasts depend on level of cancer transformation[J].Biochimica et biophysica acta. Molecular cell research,20145(5):1013-1019. |
| [27] | Kim, M.S.;Kim, J.;Lee, W.;Cho, S.-J.;Oh, J.-M.;Lee, J.-Y.;Baek, S.;Kim, Y.J.;Sim, T.S.;Lee, H.J.;Jung, G.-E.;Kim, S.-I.;Park, J.-M.;Oh, J.H.;Gurel, O.;Lee, S.S.;Lee, J.-G..A trachea-inspired bifurcated microfilter capturing viable circulating tumor cells via altered biophysical properties as measured by atomic force microscopy[J].Small,201318(18):3103-3110. |
| [28] | Liu, Yang;Wang, Zuobin;Wang, Xinyue.AFM-Based Study of Fullerenol (C-60(OH)(24))-Induced Changes of Elasticity in Living SMCC-7721 Cells[J].Journal of the mechanical behavior of biomedical materials,2015:65-74. |
| [29] | Gaspar, Diana;Freire, Joao M.;Pacheco, Teresa R.;Barata, Joao T.;Castanho, Miguel A. R. B..Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics[J].Biochimica et biophysica acta. Molecular cell research,20152(2):308-316. |
| [30] | Li, Mi;Xiao, Xiubin;Zhang, Weijing;Liu, Lianqing;Xi, Ning;Wang, Yuechao.AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells[J].Cellular immunology,20142(2):233-244. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%