利用透射电子显微镜(TEM)、X射线粉末衍射(XRD)、紫外-可见吸收光谱(UV-Vis)、傅里叶变换红外光谱(FT-IR)、同步荧光和三维荧光光谱,研究了pH=7.40条件下纳米ZnS与明胶蛋白质的相互作用.FT-IR光谱表明,ZnS与明胶中酰胺基的氮和氧原子及羧基氧可能发生键合生成了水溶性ZnS/明胶纳米生物复合物;ZnS与明胶的键合对明胶分子的内源荧光有明显的猝灭作用,其猝灭机制为静态猝灭.根据修正的Scatchard方程计算了不同温度下复合物的生成常数及反应热力学参数,结果表明反应是自发进行的吸热过程,且为熵驱动.同步荧光和三维荧光光谱表明纳米ZnS引起了明胶蛋白质构象的改变.研究结果为探究此类纳米生物复合物的制备及其在生物方面的应用提供了重要信息.
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