本文构建了一种基于苯并吲哚季铵盐结构的荧光探针用于检测SO2衍生物.该荧光探针能够快速、灵敏、高选择性地检测HSO3和SO32-,并显示出颜色和荧光变化双重响应.其比率荧光强度(I462/I588)与HSO3-的浓度(0～16 μmol/L)之间具有良好的线性关系,检测限低至12 nmol/L.1 HNMR表明该探针的响应机制为1,4-亲核加成反应.激光共聚焦荧光成像结果表明,CZBI具有良好的细胞膜通透性,并且可以通过比率荧光成像实现对细胞内SO2衍生物的监测.
In this study,we report a ratiometric fluorescent probe (CZBI) for sulfur dioxide (SO2) derivatives based on the conjugate of carbazole and benzo[e]indolium,which displays colorimetric and ratiometric fluorescence dual response to HSO3-/SO32-.The probe can quantitatively detect HSO3 with high specificity,fast response (within 40 s) as well as low detection limit (12 nmol/L).The fluorescence intensity ratios were linearly related to the concentrations of bisulfite ranging from 0 to 16 μmol/L.A 1,4-nucleophilic addition reaction was proposed for the sensing mechanism of this probe,which was confirmed by 1HNMR spectra.Fluorescence imaging of HeLa cells indicated that CZBI could be used for monitoring the intrinsically generated intracellular SO2 derivatives in living cells by ratiometric fluorescence imaging.
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