小分子α-酮戊二酸(α-KA)是人体新陈代谢的一种十分重要的产物,与多种疾病存在直接或者间接的关系,可以对临床诊断提供帮助.以BODIPY单元作为能量给体、罗丹明单元作为能量受体,利用二者存在重叠的光谱,本文设计了一种基于罗丹明和BODIPY单元的荧光共振能量转移(FRET)探针,通过将罗丹明与BODIPY的衍生物进行桥联,得到能够检测α-酮戊二酸的荧光比率型探针RDM-BO.研究表明,探针RDM-BO本身的荧光光谱与BODIPY单元类似,当探针与α-酮戊二酸发生反应时,RDM-BO中的罗丹明单元会发生开环反应,使分子的共轭发生变化,同时产生新的荧光峰,检测效果显著,检测限可达到3.14μmol·L-1.
Small molecule α-ketoglutaric acid (α-KA) is a very important product of metabolism in human body,which exhibits direct or indirect relation with many kinds of diseases.A fluorescence resonance energy transfer (FRET) ratiometric probe for α-KA containging rhodamine and BODIPY has been synthesized.As the energy donor,the fluorescence spectrum of BODIPY matches well with the absorption spectrum of rhodamine which is chosen as the energy acceptor.Research shows that the orginal fluorescence spectrum of probe RDM-BO is similar with BODIPY.While the α-KA induces ring-open reacntion with rhodamine unit of RDM-BO,the molecular conjugate is changed,resulting in new fluorescence peaks.The detection limit could reach 3.14 tmol · L-1.
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