为了研究雄烯二酮（4?androstene?3，17?dione， AED）长期暴露对食蚊鱼（ Gambusia affinis）肝脏7?乙氧基?3?异酚恶唑脱乙基酶（EROD）和谷胱甘肽硫转移酶（GST）的影响，分别利用不同浓度的AED（0．14、1．4、14、140 nmol·L－1和420 nmol·L－1）对食蚊鱼仔鱼进行180 d的水浴暴露，同时设丙酮和空白对照组，分别于第180天取样后测定肝脏的EROD和GST活性．结果表明，与对照组相比，AED对食蚊鱼雌性幼鱼的体重和体长均起到显著的抑制作用，对食蚊鱼雄性幼鱼的体重和体长的影响不明显，只有最高浓度起抑制作用． AED对食蚊鱼幼鱼EROD的抑制作用与暴露浓度呈显著的剂量关系．各浓度组AED对雌性食蚊鱼幼鱼和雄性食蚊鱼幼鱼GST活性的影响大致呈现出倒“U”曲线的趋势．
Two main anti?oxidation enzymes, 7?ethoxyresorufin?O?deethylase ( EROD ) and glutathione S?transferase( GST) , play an important role in the transformation and metabolization of toxicants. In this study, the effect of androstenedione ( AED ) on EROD and GST in the liver of Gambusia affinis was investigated. Both male and female mosquitofish fry were exposed to 0.14、1.4、14、140 nmol·L-1 and 420 nmol·L-1 AED for 180 d by using the hydrostatic bath method, and EROD and GST enzyme activities in fish liver were detected, respectively. The results showed that within a certain concentration range of AED, there was dose ?effect relationship between EROD activity and AED concentration. The GST activity was induced significantly at lower doses (1.4,14, 140 nmol·L-1 ) , but was inhibited at higher doses ( 420 nmol·L-1 ) .
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