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研究了钙源种类对脲解型微生物诱导碳酸钙沉积的生物-化学过程的影响。利用电位分析法实时测试了沉积过程中钙离子、铵离子及p H 值的变化,并利用显微计数对细菌浓度进行了监测。采用扫描电镜(SEM)、X射线衍射(XRD)和分析红外光谱(IR)对沉积产物进行了研究。结果表明,不同钙源环境下脲解型微生物诱导矿化沉积都存在化学沉淀、微生物诱导矿化沉积和沉淀完全3个阶段;有机钙源环境下细菌的产矿动力比在无机钙源中高,且两种钙源所获得的方解石晶体沉积物在形貌上差异显著。

The effects of calcium source type on the bio-chemical processes of ureolytic microbiologically-induced calcium carbonate precipitation was investigated in this paper.By using potentiometric analysis,calcium ions concentration,ammonium ions concentration,and pH value were measured.Cell density was also monitored by microscopic counting method.Sediments were characterized by scanning electron microscopy,X-ray diffraction, and infrared spectroscopy.The results showed that the processes of microbiologically-induced calcium carbonate precipitation consists the abiotic precipitation stage,the microbiologically-induced calcium carbonate precipitati-on stage,and the calcium ions depletion stage,regardless of the calcium source type.The efficiency of bacterial-ly-induce mineralized precipitation in organic calcium source environment is higher than that in inorganic calcium source environment.Significant morphological difference of precipitated calcites from two types of calcium sources was detected.

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