采用稀释分离法,从山西中条山铜矿的酸性矿坑水中分离出一株铁氧化细菌,命名为ZTS菌株.该菌株为革兰氏阴性细菌,螺旋状或弧状,最适生长温度为30 ℃,最适生长pH值为2.5,对低浓度的酵母浸出物较敏感.形态学和生理学分析表明,ZTS菌株可能属于钩端螺旋菌属的成员;16S rDNA序列的系统发育分析表明,该菌株与氧化亚铁钩端螺旋菌典型菌株ATCC 49879位于系统发育树的同一分支,相似度为100%,从而证实ZTS菌株属于氧化亚铁钩端螺旋菌.考虑到当前的商业利益,考察了Cu2+、Co2+ 和Ni2+对ZTS菌株氧化亚铁能力的影响.结果表明:3种金属对ZTS菌株的毒性强弱依次为Co2+＞Cu2+＞Ni2+.就Ni2+抗性能力而言,ZTS菌株能够耐受Ni2+的浓度高达160 mmol/L,这比已报道的氧化亚铁钩端螺旋菌的Ni2+抗性能力要强得多,这种能力可能与它的遗传特性有关.
参考文献
| [1] | Donovan P. Kelly;Ann P. Wood .Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Halothiobacillus gen. nov. and Thermithiobacillus gen. nov.[J].International Journal of Systematic and Evolutionary Microbiology,2000(Pt.2):511-516. |
| [2] | SAND W;GEHRKE T;HALLMANN R;RHODE K,SOBOTKE B,WENTZIEN S.In-situ bioleaching of metal sulfides:The importance of Leptospirillum ferrooxidans[A].Warrendale,Pennsylvaia,1993:15-27. |
| [3] | Vera M;Pagliai F;Guiliani N;Jerez CA .The chemolithoautotroph Acidithiobacillus ferrooxidans can survive under phosphate-limiting conditions by expressing a C-P lyase operon that allows it to grow on phosphonates[J].Applied and Environmental Microbiology,2008(6):1829-1835. |
| [4] | M.Nemati;S.T.L.Harrison .A comparative study on thermophilic and mesophilic biooxidation of ferrous iron[J].Minerals Engineering,2000(1):19-24. |
| [5] | Hong-mei Li;Jia-jun Ke .Influence of Ni~(2+) and Mg~(2+) on the growth and activity of Cu~(2+)-adapted Thiobacillus ferrooxidans[J].Hydrometallurgy,2001(3):151-156. |
| [6] | LEDUC L G;FERRONI G D .The chemolithotrophic bacterium Thiobacillus ferrooxidans[J].FEMS Microbiology Reviews,1994,14:103-120. |
| [7] | LEDUC L G;FERRONI G D;TREVORS J T .Resistance to heavy metals in different strains of Thiobacillus ferrooxidans[J].World Journal of Microbiology and Biotechnology,1997,13:453-455. |
| [8] | Das A.;Modak J.M. .TECHNICAL NOTE STUDIES ON MULTI-METAL ION TOLERANCE OF THIOBACILLUS FERROOXIDANS[J].Minerals Engineering,1997(7):743-749. |
| [9] | Novo MT;da Silva-AC;Moreto R;Cabral PC;Costacurta A;Garcia O Jr;Ottoboni LM .Thiobacillus ferrooxidans response to copper and other heavy metals: growth, protein synthesis and protein phosphorylation.[J].Antonie van Leeuwenhoek: Journal of Microbiology and serology,2000(2):187-195. |
| [10] | S.D. Kim;J.E. Bae;H.S. Park;D.K. Cha .Bioleaching of cadmium and nickel from synthetic sediments by Acidithiobacillus fevrooxidans[J].Environmental geochemistry and health,2005(3):229-235. |
| [11] | LOMBARDI A T;GARCIA O;MOZETO A A .Bioleaching of metals from anaerobic sewage sludge:Effects of total solids,leaching microorganisms,and energy source[J].Journal of Environmental Science and Health Part A:Toxic/Hazardous Substances & Environmental Engineering,2001,36:793-806. |
| [12] | Bor-Yann Chen;Hsuan-Liang Liu;Yun-Wen Chen;Yang-Chu Cheng .Dose-response assessment of metal toxicity upon indigenous Thiobacillus thiooxidans BC1[J].Process biochemistry,2004(6):735-745. |
| [13] | BARREIRA R P R;VILLAR L D;GARCIA O .Tolerance to copper and zinc of Acidithiobacillus thiooxidans isolated from sewage sludge[J].World Journal of Microbiology and Biotechnology,2005,21:89-91. |
| [14] | Konishi Y;Yoshida S;Asai S .BIOLEACHING OF PYRITE BY ACIDOPHILIC THERMOPHILE ACIDIANUS BRIERLEYI[J].Biotechnology and Bioengineering,1995(6):592-600. |
| [15] | VITAYA V B;LOIZUMI J;TODA K A .Kinetic assessment of substantial oxidation by Sulfolobus acidocaldarius in pyrite dissolution[J].Journal of Fermentation and Bioengineering,1994,77(05):528-534. |
| [16] | 丁建南,朱若林,康健,张成桂,吴学玲,邱冠周.喜温嗜酸硫杆菌YN12菌株的鉴定及其镉抗性能[J].中国有色金属学报,2008(02):342-348. |
| [17] | NORRIS P R;PARROTT L;MARSH R M .Moderately thermophilic mineral-oxidizing bacteria[J].Biotechnology & Bioengineering Symposium,1986,16:253-262. |
| [18] | 高健,康健,吴学玲,徐競,李邦梅,邱冠周.Fe2+对嗜铁钩端螺旋菌L. ferriphilum生长活性的影响[J].中国有色金属学报,2008(01):159-162. |
| [19] | GAO J;ZHANG C G;WU X L;WANG H H,QIU G Z .Isolation and identification of a strain of L.ferriphium from an acid mine adrainage site[J].ANNALS OF MICROBIOLOGY,2007,57(02):171-176. |
| [20] | VOGEL A I.A textbook of quantitative chemical analysis[M].London:Longmam,1962:309-319. |
| [21] | GüRTLER V;STANISICH V A .New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region[J].Journal of Microbiology,1996,142:3-16. |
| [22] | Tamura K;Dudley J;Nei M;Kumar S .MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0[J].Molecular biology and evolution,2007(8):1596-1599. |
| [23] | SAITOU N;NEI M .The neighbor-joining method:A new method for reconstructing phylogenetic trees[J].Molecular Biology and Evolution,1987,4:406-425. |
| [24] | Hans Hippe .Leptospirillum gen. nov. (ex Markosyan 1972), nom. rev., including Leptospirillum ferrooxidans sp. nov. (ex Markosyan 1972), nom. rev. and Leptospirillum thermoferrooxidans sp. nov. (Golovacheva et al. 1992)[J].International Journal of Systematic and Evolutionary Microbiology,2000(2):501-503. |
| [25] | Nicolette J. Coram;Douglas E. Rawlings .Molecular Relationship between Two Groups of the Genus Leptospirillum and the Finding that Leptospirillum ferriphilum sp. nov. Dominates South African Commercial Biooxidation Tanks That Operate at 40 ℃[J].Applied and Environmental Microbiology,2002(2):838-845. |
| [26] | Tyson GW;Lo I;Baker BJ;Allen EE;Hugenholtz P;Banfield JF .Genome-directed isolation of the key nitrogen fixer Leptospirillum ferrodiazotrophum sp. nov. from an acidophilic microbial community[J].Applied and Environmental Microbiology,2005(10):6319-6324. |
| [27] | VARDANYAN N S;AKOPYAN V P .Leptospirillum-like bacteria and evaluation of their role in pyrite oxidation[J].Mikrobiologiia,2003,72(04):493-497. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%