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利用间歇式方法培养硫酸盐还原菌(SRB)并制备SRB生物膜,研究表明,随着细菌的生长,细菌代谢产物改变了介质的pH,生物膜厚度增加,膜中含菌量升高。在3%NaCl水溶液中,覆盖有不同生长期生物膜的碳钢试片的腐蚀速度有明显的差异;电子能谱对生物膜表面分析结果表明,不同生长期生物膜腐蚀产物的Fe/S比各不相同。为了验证生物膜中主要腐蚀因素,利用化学方法在试片表面沉积FeS膜;利用细菌滤膜隔离SRB,在试片表面沉积无菌腐蚀产物膜。将三种膜作腐蚀对比实验,结果表明以含菌生物膜腐蚀最重,活性腐蚀产物膜次之,化学沉积FeS膜最轻,因此引起碳钢微生物腐蚀的原因主要是生物膜下SRB代谢产生的有机酸的富集引起酸浸蚀。

The effects of biofilms of Sulfate Reducing Bacteria(SRB) on carbon steel have been studied. API RP-38 media were used to culture SRB in batch and prepare SRB biofilms. The results show that the biofilms become thicker with the growth of SRB. Moreover, corrosion potential of the electrode varies with time. While the pH of the media with SRB changes from 7.2 to 6.0, the count of SRB in biofilms increases. The corrosion rates of carbon steel coupons covered by biofilms with different growth periods in 3% NaCl solution are remarkably distinctive. EDXA results present that the components of corrosion products in thesis and a bilfilm was prepared by bilolgical method.The corrosion rates for the three sorts of film were two films. The corrosion mechanism is mainly sttributed to acid attack.

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