以木炭为碳源,分别采用回流、微波及超声等不同方法制备碳量子点(Carbon quantum dots,CQDs).比较不同方法的优劣并优化反应条件、考察不同因素对其荧光量子产率的影响.得到最佳制备方法,制得粒径较小的荧光CQDs,用钝化剂PEG2000修饰后,提高其荧光寿命和量子产率.将修饰后的CQDs应用于与牛血清白蛋白(Bovine serum albumin,BSA)的相互作用,采用紫外吸收光谱法和荧光光谱法探讨其相互作用机理.结果表明,经回流法所制CQDs的荧光量子产率最高,其与BSA之间的荧光猝灭为静态猝灭过程.
参考文献
| [1] | Zhu, H;Wang, XL;Li, YL;Wang, ZJ;Yang, F;Yang, XR .Microwave synthesis of fluorescent carbon nanoparticles with electrochemiluminescence properties[J].Chemical communications,2009(34):5118-5120. |
| [2] | Zheng, LY;Chi, YW;Dong, YQ;Lin, JP;Wang, BB .Electrochemiluminescence of Water-Soluble Carbon Nanocrystals Released Electrochemically from Graphite[J].Journal of the American Chemical Society,2009(13):4564-4566. |
| [3] | Lewinski N;Colvin V;Drezek R .Cytotoxicity of nanoparticles[J].Small,2008(1):26-49. |
| [4] | Chen LD;Liu J;Yu XF;He M;Pei XF;Tang ZY;Wang QQ;Pang DW;Li Y .The biocompatibility of quantum dot probes used for the targeted imaging of hepatocellular carcinoma metastasis.[J].Biomaterials,2008(31):4170-4176. |
| [5] | Tiwad D K;Tanaka S I;Inouye Y et al.Synthesis and characterization of anti-HER2 antibody conjugated CdSe/CdZnS quantum dots for fluorescence imaging of breast cancer cells[J].Sensors,2009,9(11):9332-9354. |
| [6] | 邱月琴,蔡继业.量子点在细胞以及体内生物中成像的研究进展[J].材料导报,2012(01):16-19. |
| [7] | PINPIN LIN;JEIN-WEN CHEN;LOUIS W. CHANG .Computational and Ultrastructural Toxicology of a Nanoparticle, Quantum Dot 705, in Mice[J].Environmental Science & Technology: ES&T,2008(16):6264-6270. |
| [8] | Geys J;Nemmar A;Verbeken E;Smolders E;Ratoi M;Hoylaerts MF;Nemery B;Hoet PH .Acute toxicity and prothrombotic effects of quantum dots: impact of surface charge.[J].Environmental health perspectives,2008(12):1607-1613. |
| [9] | Hardman R .A toxicologic review of quantum dots: toxicity depends on physicochemical and environmental factors.[J].Environmental health perspectives,2006(2):165-172. |
| [10] | 黄淮青,曾萍,韩宝福,徐淑坤.荧光碳点的合成及对酿酒酵母的毒性研究[J].无机化学学报,2012(01):13-19. |
| [11] | Sheng-Tao Yang;Xin Wang;Haifang Wang .Carbon Dots as Nontoxic and High-Performance Fluorescence Imaging Agents[J].The journal of physical chemistry, C. Nanomaterials and interfaces,2009(42):18110-18114. |
| [12] | Sandra J. Rosenthal;James McBride;Stephen J. Pennycook;Leonard C. Feldman .Synthesis, surface studies, composition and structural characterization of CdSe, core/shell and biologically active nanocrystals[J].Surface Science Reports,2007(4):111-157. |
| [13] | Xiao-Jiao Mao;Hu-Zhi Zheng;Yi-Juan Long;Juan Du;Jian-Yu Hao;Ling-Ling Wang;Dong-Bo Zhou .Study on the fluorescence characteristics of carbon dots[J].Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy,2010(2):553-557. |
| [14] | Hui Peng;Jadranka Travas-Sejdic .Simple Aqueous Solution Route to Luminescent Carbogenic Dots from Carbohydrates[J].Chemistry of Materials: A Publication of the American Chemistry Society,2009(23):5563-5565. |
| [15] | Zhao QL;Zhang ZL;Huang BH;Peng J;Zhang M;Pang DW .Facile preparation of low cytotoxicity fluorescent carbon nanocrystals by electrooxidation of graphite[J].Chemical communications,2008(41):5116-5118. |
| [16] | Liu C J;Zhang P;Zhai X Y et al.Nano-carrier for gene delivery and bioimaging based on carbon dots with PEI-passivation enhanced ttuorescence[J].Biomaterials,2012,33(13):3604-3613. |
| [17] | Yongqiang Dong;Ruixue Wang;Hao Li .Polyamine-functionalized carbon quantum dots for chemical sensing[J].Carbon: An International Journal Sponsored by the American Carbon Society,2012(8):2810-2815. |
| [18] | S. C. Ray;Arindam Saha;Nikhil R. Jana .Fluorescent Carbon Nanoparticles: Synthesis, Characterization, and Bioimaging Application[J].The journal of physical chemistry, C. Nanomaterials and interfaces,2009(43):18546-18551. |
| [19] | Sun Y R;Zhou B;Lin Y et al.Quantum-sized carbon dots for bright and colorful photolumineseenee[J].Journal of the American Chemical Society,2006,128:7756-7757. |
| [20] | Cao L;Wang X;Meziani MJ;Lu FS;Wang HF;Luo PJG;Lin Y;Harruff BA;Veca LM;Murray D .Carbon dots for multiphoton bioimaging[J].Journal of the American Chemical Society,2007(37):11318-11319. |
| [21] | Haipeng Liu;Tao Ye;Chengde Mao .Fluorescent Carbon Nanoparticles Derived from Candle Soot[J].Angewandte Chemie,2007(34):6473-6475. |
| [22] | Jigang Zhou;Christina Booker;Ruying Li .An Electrochemical Avenue to Blue Luminescent Nanocrystals from Multiwalled Carbon Nanotubes (MWCNTs)[J].Journal of the American Chemical Society,2007(4):744-745. |
| [23] | Sheila N. Baker;Gary A. Baker .Luminescent Carbon Nanodots: Emergent Nanolights[J].Angewandte Chemie,2010(38):6726-6744. |
| [24] | Chakraborty B;Basu S .Interaction of BSA with proflavin: A spectroscopic approach[J].Journal of Luminescence: An Interdisciplinary Journal of Research on Excited State Processes in Condensed Matter,2009(1):34-39. |
| [25] | 徐金昌 .谷胱甘肽稳定的CdTe量子点的制备及其标记生物分子研究[D].四川农业大学,2009. |
| [26] | 胡胜亮,白培康,曹士锐,孙景.脉冲激光制备发光碳纳米颗粒[J].高等学校化学学报,2009(08):1497-1500. |
| [27] | 骆克来,张群,任丽英.荧光法研究纳米TiO2与BSA的相互作用[J].安庆师范学院学报（自然科学版）,2009(02):55-57,60. |
| [28] | Matyus L;Szollosi J;Jenei A .Steady-state fluorescence quenching applications for studying protein structure and dynamics[J].Journal of Photochemistry and Photobiology, B. Biology: Official Journal of the European Society for Photobiology,2006(3):223-236. |
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