利用介孔材料纳米孔道作为微反应器,将带正电荷的Cd离子与介孔二氧化硅(SiO2)表面带有负电荷的-SiOH相互静电吸附生成Cd-O-Si,然后采用低温方法使其与SeSO32-反应,缓慢生长,形成CdSe纳米晶.XRD和高倍透射电镜说明介孔孔道中生成了CdSe纳米颗粒.利用CdSe/SiO在UV激发下产生光电流的特性,用得到的CdSe/SiO复合微球作载体固定辣根过氧化物酶HRP,在OTE涂上一层HRP/CdSe/SiO2膜,应用到H2O2生物传感器的检测.过氧化物酶-电极系统内,在紫外光(UV)365nm波长照射下,由CdSe/SiO2产生的光电子和电极提供的电子同时对H2O2进行还原,产生的空穴被CdSe和SiO2的界面捕获,提高了反应速度.
参考文献
| [1] | Xiaogang Peng;Michael C. Schlamp;Andreas V. Kadavanich .Epitaxial Growth of Highly Luminescent CdSe/CdS Core/Shell Nanocrystals with Photostability and Electronic Accessibility[J].Journal of the American Chemical Society,1997(30):7019-7029. |
| [2] | Empedocles S A;Bawendi M G .Spectroscopy of single CdSe nanocrystallites[J].Accounts of Chemical Research,1999,32:389. |
| [3] | Thoma, SG;Sanchez, A;Provencio, PP;Abrams, BL;Wilcoxon, JP .Synthesis, optical properties, and growth mechanism of blue-emitting CdSe nanorods[J].Journal of the American Chemical Society,2005(20):7611-7614. |
| [4] | Hiramatsu H.;Osterloh FE. .pH-controlled assembly and disassembly of electrostatically linked CdSe-SiO2 and Au-SiO2 nanoparticle clusters[J].Langmuir: The ACS Journal of Surfaces and Colloids,2003(17):7003-7011. |
| [5] | Gao F;Chino N;Naik S P et al.Properties of nano-ZnO fabricated in mesoporous silica film[J].Materials Letters,2007,61(14-15):3179. |
| [6] | 洪伟杰,张朝晖,芦国营.辣根过氧化物酶的结构与作用机制[J].生命的化学,2005(01):33-36. |
| [7] | Liu D;Kamat P V .Electrochemical rectification in CdSe+TiO2 coupled semiconductor films[J].Journal of Electroanalytical Chemistry and Interfacial Electrochemistry,1993,347(1-2):451. |
| [8] | Istvan Robel;Vaidyanathan Subramanian;Masaru Kuno;Prashant V.Kamat .Quantum Dot Solar Cells.Harvesting Light Energy with CdSe Nanocrystals Molecularly Linked to Mesoscopic TiO_2 Films[J].Journal of the American Chemical Society,2006(7):2385-2393. |
| [9] | Ferri T;Poscia A;Santucci R .Direct electrochemisty of a membrane entrapped horseradish peroxidase part Ⅱ:Flowing amperometric detection of H2O2 and other analytes[J].Bioelectrochemistry and Bioenergetics,1998,45(02):221. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%